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High-throughput mapping of long-range neuronal projection using in situ sequencing

View ORCID ProfileXiaoyin Chen, Huiqing Zhan, Justus M Kebschull, Yu-Chi Sun, Anthony M Zador
doi: https://doi.org/10.1101/294637
Xiaoyin Chen
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Huiqing Zhan
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Justus M Kebschull
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
2Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA
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Yu-Chi Sun
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Anthony M Zador
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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  • For correspondence: zador@cshl.edu
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Summary

Understanding neural circuits requires deciphering the interactions of myriad cell types defined by connectivity, spatial organization, gene expression, and other properties. Resolving these cell types requires both single neuron resolution and high throughput, a combination that is challenging to achieve with conventional anatomical methods. Here we introduce BARseq, a method for mapping the projections of thousands of spatially resolved neurons by RNA barcoding. We used BARseq to determine the projections of 1309 neurons in mouse auditory cortex to 11 targets. We observed 264 distinct projection patterns. Hierarchical clustering confirmed the major classical classes of projection neurons, as well as further subdivisions within each class. We further combined BARseq with in situ detection of mRNA to relate neuronal gene expression with projections. By bridging high-throughput neuroanatomy with high spatial resolution, BARseq provides a path towards a systematic multi-modal description of the nervous system.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 31, 2018.
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High-throughput mapping of long-range neuronal projection using in situ sequencing
Xiaoyin Chen, Huiqing Zhan, Justus M Kebschull, Yu-Chi Sun, Anthony M Zador
bioRxiv 294637; doi: https://doi.org/10.1101/294637
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High-throughput mapping of long-range neuronal projection using in situ sequencing
Xiaoyin Chen, Huiqing Zhan, Justus M Kebschull, Yu-Chi Sun, Anthony M Zador
bioRxiv 294637; doi: https://doi.org/10.1101/294637

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