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A multiplexed homology-directed DNA repair assay reveals the impact of ~1,700 BRCA1 variants on protein function

Lea M. Starita, Muhtadi M. Islam, Tapahsama Banerjee, Aleksandra I. Adamovich, Justin Gullingsrud, Stanley Fields, Jay Shendure, Jeffrey D. Parvin
doi: https://doi.org/10.1101/295279
Lea M. Starita
1Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
2Brotman Baty Institute for Precision Medicine, Seattle, Washington, USA.
7These authors contributed equally to this work.
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Muhtadi M. Islam
3Department of Biomedical Informatics, The Ohio State University, Columbus, Ohio, USA.
7These authors contributed equally to this work.
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Tapahsama Banerjee
3Department of Biomedical Informatics, The Ohio State University, Columbus, Ohio, USA.
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Aleksandra I. Adamovich
3Department of Biomedical Informatics, The Ohio State University, Columbus, Ohio, USA.
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Justin Gullingsrud
1Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
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Stanley Fields
1Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
4Department of Medicine, University of Washington, Seattle, Washington, USA.
5Howard Hughes Medical Institute, Seattle, Washington, USA.
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Jay Shendure
1Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
2Brotman Baty Institute for Precision Medicine, Seattle, Washington, USA.
5Howard Hughes Medical Institute, Seattle, Washington, USA.
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  • For correspondence: Jeffrey.Parvin@osumc.edu shendure@u.washington.edu
Jeffrey D. Parvin
3Department of Biomedical Informatics, The Ohio State University, Columbus, Ohio, USA.
6The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USA.
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  • For correspondence: Jeffrey.Parvin@osumc.edu shendure@u.washington.edu
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Article Information

doi 
https://doi.org/10.1101/295279
History 
  • April 5, 2018.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Lea M. Starita1,2,7,
  2. Muhtadi M. Islam3,7,
  3. Tapahsama Banerjee3,
  4. Aleksandra I. Adamovich3,
  5. Justin Gullingsrud1,
  6. Stanley Fields1,4,5,
  7. Jay Shendure*,1,2,5 and
  8. Jeffrey D. Parvin*,3,6
  1. 1Department of Genome Sciences, University of Washington, Seattle, Washington, USA.
  2. 2Brotman Baty Institute for Precision Medicine, Seattle, Washington, USA.
  3. 3Department of Biomedical Informatics, The Ohio State University, Columbus, Ohio, USA.
  4. 4Department of Medicine, University of Washington, Seattle, Washington, USA.
  5. 5Howard Hughes Medical Institute, Seattle, Washington, USA.
  6. 6The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, USA.
  7. 7These authors contributed equally to this work.
  1. ↵*Correspondence should be addressed to J.D.P (Jeffrey.Parvin{at}osumc.edu) or J.S. (shendure{at}u.washington.edu).
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Posted April 05, 2018.
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A multiplexed homology-directed DNA repair assay reveals the impact of ~1,700 BRCA1 variants on protein function
Lea M. Starita, Muhtadi M. Islam, Tapahsama Banerjee, Aleksandra I. Adamovich, Justin Gullingsrud, Stanley Fields, Jay Shendure, Jeffrey D. Parvin
bioRxiv 295279; doi: https://doi.org/10.1101/295279
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A multiplexed homology-directed DNA repair assay reveals the impact of ~1,700 BRCA1 variants on protein function
Lea M. Starita, Muhtadi M. Islam, Tapahsama Banerjee, Aleksandra I. Adamovich, Justin Gullingsrud, Stanley Fields, Jay Shendure, Jeffrey D. Parvin
bioRxiv 295279; doi: https://doi.org/10.1101/295279

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