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Mechanosensation Dynamically Coordinates Polar Growth and Cell Wall Assembly to Promote Cell Survival

Valeria Davì, Hirokazu Tanimoto, Dmitry Ershov, Armin Haupt, Henry De Belly, Remi Le Borgne, Etienne Couturier, Arezki Boudaoud, View ORCID ProfileNicolas Minc
doi: https://doi.org/10.1101/299008
Valeria Davì
1Institut Jacques Monod, CNRS UMR7592 and Université Paris Diderot, 15 rue Hélène Brion, 75205 Paris Cedex 13, France
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Hirokazu Tanimoto
1Institut Jacques Monod, CNRS UMR7592 and Université Paris Diderot, 15 rue Hélène Brion, 75205 Paris Cedex 13, France
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Dmitry Ershov
1Institut Jacques Monod, CNRS UMR7592 and Université Paris Diderot, 15 rue Hélène Brion, 75205 Paris Cedex 13, France
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Armin Haupt
1Institut Jacques Monod, CNRS UMR7592 and Université Paris Diderot, 15 rue Hélène Brion, 75205 Paris Cedex 13, France
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Henry De Belly
1Institut Jacques Monod, CNRS UMR7592 and Université Paris Diderot, 15 rue Hélène Brion, 75205 Paris Cedex 13, France
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Remi Le Borgne
1Institut Jacques Monod, CNRS UMR7592 and Université Paris Diderot, 15 rue Hélène Brion, 75205 Paris Cedex 13, France
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Etienne Couturier
2Laboratoire Matières et Systèmes Complexes, UMR7057, CNRS and Université Paris Diderot, Paris, France
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Arezki Boudaoud
3Reproduction et Développement des Plantes, Univ. Lyon, INRA, CNRS, ENS de Lyon, UCB Lyon I, 46 Allée d’Italie, 69364 Lyon Cedex 07, France
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  • For correspondence: arezki.boudaoud@ens-lyon.fr nicolas.minc@ijm.fr
Nicolas Minc
1Institut Jacques Monod, CNRS UMR7592 and Université Paris Diderot, 15 rue Hélène Brion, 75205 Paris Cedex 13, France
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  • ORCID record for Nicolas Minc
  • For correspondence: arezki.boudaoud@ens-lyon.fr nicolas.minc@ijm.fr
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SUMMARY

How growing cells cope with size expansion while ensuring mechanical integrity is not known. In walled cells, such as those of microbes and plants, growth and viability are both supported by a thin and rigid encasing cell wall (CW). We deciphered the dynamic mechanisms controlling wall surface assembly during cell growth, using a novel sub-resolution microscopy approach to monitor CW thickness in live rod-shaped fission yeast cells. We found that polar cell growth yielded wall thinning, and that thickness negatively influenced growth. Thickness at growing tips exhibited oscillating behavior with thickening phases followed by thinning phases, indicative of a delayed feedback promoting thickness homeostasis. This feedback was mediated by mechanosensing through the cell wall integrity pathway, which probes strain in the wall to adjust synthase localization and activity to surface growth. Mutants defective in thickness homeostasis lysed by rupturing the wall demonstrating its essential role for walled cell survival.

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Posted April 10, 2018.
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Mechanosensation Dynamically Coordinates Polar Growth and Cell Wall Assembly to Promote Cell Survival
Valeria Davì, Hirokazu Tanimoto, Dmitry Ershov, Armin Haupt, Henry De Belly, Remi Le Borgne, Etienne Couturier, Arezki Boudaoud, Nicolas Minc
bioRxiv 299008; doi: https://doi.org/10.1101/299008
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Mechanosensation Dynamically Coordinates Polar Growth and Cell Wall Assembly to Promote Cell Survival
Valeria Davì, Hirokazu Tanimoto, Dmitry Ershov, Armin Haupt, Henry De Belly, Remi Le Borgne, Etienne Couturier, Arezki Boudaoud, Nicolas Minc
bioRxiv 299008; doi: https://doi.org/10.1101/299008

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