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The polar localization hub protein PopZ restrains adaptor dependent ClpXP proteolysis in Caulobacter crescentus

Kamal K Joshi, Peter Chien
doi: https://doi.org/10.1101/301606
Kamal K Joshi
University of Massachusetts Amherst;
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Peter Chien
UMass Amherst
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  • For correspondence: pchien@biochem.umass.edu
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Abstract

In Caulobacter crescentus, timely degradation of several proteins by the ClpXP protease is critical for proper cell cycle progression. During the cell cycle, the ClpXP protease, the substrate CtrA and many other proteins are localized to the stalked pole dependent on a polar interaction hub composed of PopZ protein oligomers. Prior work suggests that the localization of ClpXP, protease substrates, and cofactors is needed for recognition of substrates such as CtrA by ClpXP. Here, we formally test this hypothesis by examining the role of PopZ in ClpXP activity and find surprisingly that CtrA degradation is enhanced in cells lacking polar localization due to loss of PopZ. The ClpXP adaptor CpdR is required for this enhanced degradation of CtrA and other adaptor-dependent substrates, but adaptor-independent substrate degradation is not affected upon loss of PopZ. We find that overexpression of PopZ also leads to faster degradation of CtrA, but is likely due to nonphysiologically relevant recognition of CtrA by ClpXP alone as loss of CpdR does not affect this enhancement. Our main conclusion is that loss of PopZ, and therefore loss of polar localization, does not result in the loss of ClpXP regulated proteolysis, as would be predicted from a model which requires polar localization of ClpXP for its activation. Rather, our data point to a model where PopZ normally restrains ClpXP proteolysis by promoting the inactivation of the CpdR adaptor, likely through the phosphorylation activity of the CckA kinase.

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Posted April 14, 2018.
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The polar localization hub protein PopZ restrains adaptor dependent ClpXP proteolysis in Caulobacter crescentus
Kamal K Joshi, Peter Chien
bioRxiv 301606; doi: https://doi.org/10.1101/301606
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The polar localization hub protein PopZ restrains adaptor dependent ClpXP proteolysis in Caulobacter crescentus
Kamal K Joshi, Peter Chien
bioRxiv 301606; doi: https://doi.org/10.1101/301606

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