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Neuromesodermal Progenitors are a Conserved Source of Spinal Cord with Divergent Growth Dynamics

Andrea Attardi, Timothy Fulton, Maria Florescu, Gopi Shah, Leila Muresan, Jan Huisken, Alexander van Oudenaarden, View ORCID ProfileBenjamin Steventon
doi: https://doi.org/10.1101/304543
Andrea Attardi
1Department of Genetics, University of Cambridge, Cambridge, UK.
2Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
3STEBICEF Department, Università degli Studi di Palermo, Palermo, Italy
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Timothy Fulton
1Department of Genetics, University of Cambridge, Cambridge, UK.
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Maria Florescu
4Hubrecht Institute, Utrecht, The Netherlands
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Gopi Shah
2Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
5Cancer Research UK, Cambridge Institute, Cambridge, UK
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Leila Muresan
6Cambridge Advanced Imaging Center, Cambridge, UK
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Jan Huisken
2Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
7Morgridge Institute for Research, Madison, USA
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Alexander van Oudenaarden
4Hubrecht Institute, Utrecht, The Netherlands
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Benjamin Steventon
1Department of Genetics, University of Cambridge, Cambridge, UK.
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  • ORCID record for Benjamin Steventon
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Abstract

During gastrulation, embryonic cells become specified into distinct germ layers. In mouse, this continues throughout somitogenesis from a population of bipotent stem cells called neuromesodermal progenitors (NMps). However, the degree self-renewal is associated with NMps in the fast-developing zebrafish embryo is unclear. With a genetic clone tracing method, we labelled early embryonic progenitors and find a strong clonal similarity between spinal cord and mesoderm tissues. We then followed individual cell lineages by light-sheet imaging and reveal a common neuromesodermal lineage contribution to a subset of spinal cord tissue across the anterior-posterior body axis. An initial population subdivides at mid gastrula stages and is directly allocated to neural and mesodermal compartments during gastrulation. A second population in the tailbud undergoes delayed allocation to contribute to the neural and mesodermal compartment only at late somitogenesis. We suggest that NMps undergo vastly different rates of differentiation and growth in a species-specific manner.

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Posted April 19, 2018.
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Neuromesodermal Progenitors are a Conserved Source of Spinal Cord with Divergent Growth Dynamics
Andrea Attardi, Timothy Fulton, Maria Florescu, Gopi Shah, Leila Muresan, Jan Huisken, Alexander van Oudenaarden, Benjamin Steventon
bioRxiv 304543; doi: https://doi.org/10.1101/304543
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Neuromesodermal Progenitors are a Conserved Source of Spinal Cord with Divergent Growth Dynamics
Andrea Attardi, Timothy Fulton, Maria Florescu, Gopi Shah, Leila Muresan, Jan Huisken, Alexander van Oudenaarden, Benjamin Steventon
bioRxiv 304543; doi: https://doi.org/10.1101/304543

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