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Screening, Characterization and Optimization of antibacterial peptides, produced by Bacillus safensis strain MK-12 isolated from waste dump soil KP, Pakistan

Sajid Iqbal, Muhammad Qasim, Farida Begum, Hazir Rahman, Imran Sajid
doi: https://doi.org/10.1101/308205
Sajid Iqbal
1Department of Microbiology, Kohat University of Science and Technology, Kohat KP Pakistan/ National University of sciences and Technology, Islamabad Pakistan
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  • For correspondence: sajidiqbalmb44@yahoo.com
Muhammad Qasim
2Department of Microbiology, Kohat University of Science and Technology, Kohat KP Pakistan
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Farida Begum
3Department of Biochemistry, Abdul Wali Khan University Mardan, KP Pakistan
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Hazir Rahman
4Department of Microbiology, Abdul Wali Khan University Mardan, KP Pakistan
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Imran Sajid
5Department of Microbiology and Molecular Genetics (MMG), University of the Punjab, Lahore Pakistan
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Abstract

Aims The current study was designed to isolate, screen and identify the indigenous soil antibacterial exhibiting bacteria (AEB) and effect of various parameters on growth of AEB and antibacterial peptides production.

Methods and results The soil isolates were screened for antagonistic activity against a set of ATCC and local MDR human pathogenic bacterial strains. The antibacterial compound was protein in nature, exhibited no haemolysis and molecular weight was less than 20 KDa. The potential AEB isolate was identified by morphology, biochemical testing and by 16S rRNA gene sequencing as B. safensis MK-12. Growth and antibacterial activity was optimized for B. safensis strain MK-12, exhibited maximum growth as well as antibacterial activity after 48 hours of incubation at pH 8, 30 °C in shaking incubator when fermented in optimized medium.

Conclusion The current study results indicate that indigenous soil is rich source of AEB and could be a promising source of antimicrobial compounds to fight against MDR bacteria in future.

Significance and impact This is the first scientific report on soil bacteria from northern region of Pakistan as per our knowledge. Therefore, screening of soil bacteria for antibacterial activity from unexplored area may contribute towards new antibiotic. Selected soil strain in the current study exhibited promising antibacterial activity against human pathogenic MDR bacterial strains.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 25, 2018.
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Screening, Characterization and Optimization of antibacterial peptides, produced by Bacillus safensis strain MK-12 isolated from waste dump soil KP, Pakistan
Sajid Iqbal, Muhammad Qasim, Farida Begum, Hazir Rahman, Imran Sajid
bioRxiv 308205; doi: https://doi.org/10.1101/308205
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Screening, Characterization and Optimization of antibacterial peptides, produced by Bacillus safensis strain MK-12 isolated from waste dump soil KP, Pakistan
Sajid Iqbal, Muhammad Qasim, Farida Begum, Hazir Rahman, Imran Sajid
bioRxiv 308205; doi: https://doi.org/10.1101/308205

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