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Olfactory marker protein regulates refinement of the olfactory glomerular map

View ORCID ProfileDinu F. Albeanu, Allison C. Provost, Prateek Agarwal, Edward Soucy, View ORCID ProfileVenkatesh N. Murthy
doi: https://doi.org/10.1101/309401
Dinu F. Albeanu
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
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Allison C. Provost
2Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA, USA
3Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
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Prateek Agarwal
2Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA, USA
3Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
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Edward Soucy
2Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA, USA
3Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
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Venkatesh N. Murthy
2Department of Molecular & Cellular Biology, Harvard University, Cambridge, MA, USA
3Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA
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Abstract

The olfactory glomerulus is the anatomical and functional unit of the olfactory bulb, defined by convergent input from olfactory sensory neuron (OSN) axons expressing the same type of odorant receptor (OR). A key marker of mature OSNs is the olfactory marker protein (OMP), whose deletion has been associated with deficits in OSN signal transduction and odor discrimination. Here, we have investigated glomerular odor responses and anatomical architecture in mice in which one or both alleles of OMP were replaced by the fluorescent synaptic activity reporter, synaptopHluorin (OMP+/− and OMP−/− mice, respectively). Functionally heterogeneous glomeruli, that is, ones with micro-domains with distinct odor responses were rare in OMP+/− mice, but occurred frequently in OMP−/− mice. Genetic targeting of single ORs revealed that these micro-domains arise from anomalous co-innervation of individual glomeruli by OSNs expressing different ORs. The glomerular mistargeting of OSNs in the absence of OMP is restricted to a local neighborhood of a few glomerular diameters. Our studies document functional heterogeneity in sensory input within individual glomeruli and uncover its anatomical correlate, revealing an unexpected role for OMP in the formation and refinement of the glomerular olfactory map.

Footnotes

  • Disclosures/Conflict of Interest: ACP is employed by Cohen Veterans Bioscience, a non-profit public charity research organization.

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Posted May 01, 2018.
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Olfactory marker protein regulates refinement of the olfactory glomerular map
Dinu F. Albeanu, Allison C. Provost, Prateek Agarwal, Edward Soucy, Venkatesh N. Murthy
bioRxiv 309401; doi: https://doi.org/10.1101/309401
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Olfactory marker protein regulates refinement of the olfactory glomerular map
Dinu F. Albeanu, Allison C. Provost, Prateek Agarwal, Edward Soucy, Venkatesh N. Murthy
bioRxiv 309401; doi: https://doi.org/10.1101/309401

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