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DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis

Amr Nassrallah, Martin Rougée, Clara Bourbousse, Stéphanie Drevensek, Sandra Fonseca, Elisa Iniesto, Ouardia Ait-Mohamed, Anne-Flore Deton-Cabanillas, Gerald Zabulonb, Ikhlak Ahmed, David Stroebel, Vanessa Masson, Bérangère Lombard, Dominique Eeckhout, Kris Gevaert, Damarys Loew, Auguste Genovesio, Cécile Breyton, Geert de Jaeger, Chris Bowler, Vicente Rubio, Fredy Barneche
doi: https://doi.org/10.1101/309732
Amr Nassrallah
aCentro Nacional de Biotecnología (CNB-CSIC) Darwin 3, 28049 Madrid, Spain
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Martin Rougée
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
cUniversite Paris-Sud, 91405 Orsay, France
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Clara Bourbousse
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
cUniversite Paris-Sud, 91405 Orsay, France
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Stéphanie Drevensek
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
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Sandra Fonseca
aCentro Nacional de Biotecnología (CNB-CSIC) Darwin 3, 28049 Madrid, Spain
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Elisa Iniesto
aCentro Nacional de Biotecnología (CNB-CSIC) Darwin 3, 28049 Madrid, Spain
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Ouardia Ait-Mohamed
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
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Anne-Flore Deton-Cabanillas
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
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Gerald Zabulonb
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
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Ikhlak Ahmed
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
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David Stroebel
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
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Vanessa Masson
dInstitut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrometrie de Masse Proteomique, 26 rue d’Ulm, 75005 Paris, France
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Bérangère Lombard
dInstitut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrometrie de Masse Proteomique, 26 rue d’Ulm, 75005 Paris, France
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Dominique Eeckhout
eDepartment of Plant Systems Biology. VIB-Ghent University. Technologiepark 927, B-9052 Ghent, Belgium
fVIB Center for Plant Systems Biology, 9052 Ghent, Belgium
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Kris Gevaert
gDepartment of Biochemistry, Ghent University, 9000 Ghent, Belgium
hVIB Center for Medical Biotechnology, 9000 Ghent, Belgium
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Damarys Loew
dInstitut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrometrie de Masse Proteomique, 26 rue d’Ulm, 75005 Paris, France
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Auguste Genovesio
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
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Cécile Breyton
iUniversite Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale (IBS), F-3800, Grenoble, France
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Geert de Jaeger
eDepartment of Plant Systems Biology. VIB-Ghent University. Technologiepark 927, B-9052 Ghent, Belgium
fVIB Center for Plant Systems Biology, 9052 Ghent, Belgium
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Chris Bowler
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
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  • For correspondence: barneche@biologie.ens.fr vrubio@cnb.csic.es cbowler@biologie.ens.fr
Vicente Rubio
aCentro Nacional de Biotecnología (CNB-CSIC) Darwin 3, 28049 Madrid, Spain
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  • For correspondence: barneche@biologie.ens.fr vrubio@cnb.csic.es cbowler@biologie.ens.fr
Fredy Barneche
bInstitut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France.
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  • For correspondence: barneche@biologie.ens.fr vrubio@cnb.csic.es cbowler@biologie.ens.fr
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Summary

DE-ETIOLATED 1 (DET1) is an evolutionarily conserved component of the ubiquitination machinery that mediates the destabilization of key regulators of cell differentiation and proliferation in multicellular organisms. In this study, we provide evidence from Arabidopsis that DET1 is essential for the regulation of histone H2B monoubiquitination (H2Bub) over most genes by controlling the stability of a plant deubiquitination module (DUBm). In contrast with yeast and metazoan DUB modules that are associated with the large SAGA complex, the Arabidopsis DUBm only comprises three proteins (hereafter named SGF11, ENY2 and UBP22) and appears to act independently as a major H2Bub deubiquitinase activity. Our study further unveils that DET1-DDB1-Associated-1 (DDA1) protein interacts with SGF11 in vivo, linking the DET1 complex to light-dependent ubiquitin-mediated proteolytic degradation of the DUBm. Collectively, these findings uncover a signaling path controlling DUBm availability, potentially adjusting H2Bub turnover capacity to the cell transcriptional status.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 30, 2018.
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DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis
Amr Nassrallah, Martin Rougée, Clara Bourbousse, Stéphanie Drevensek, Sandra Fonseca, Elisa Iniesto, Ouardia Ait-Mohamed, Anne-Flore Deton-Cabanillas, Gerald Zabulonb, Ikhlak Ahmed, David Stroebel, Vanessa Masson, Bérangère Lombard, Dominique Eeckhout, Kris Gevaert, Damarys Loew, Auguste Genovesio, Cécile Breyton, Geert de Jaeger, Chris Bowler, Vicente Rubio, Fredy Barneche
bioRxiv 309732; doi: https://doi.org/10.1101/309732
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DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis
Amr Nassrallah, Martin Rougée, Clara Bourbousse, Stéphanie Drevensek, Sandra Fonseca, Elisa Iniesto, Ouardia Ait-Mohamed, Anne-Flore Deton-Cabanillas, Gerald Zabulonb, Ikhlak Ahmed, David Stroebel, Vanessa Masson, Bérangère Lombard, Dominique Eeckhout, Kris Gevaert, Damarys Loew, Auguste Genovesio, Cécile Breyton, Geert de Jaeger, Chris Bowler, Vicente Rubio, Fredy Barneche
bioRxiv 309732; doi: https://doi.org/10.1101/309732

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