ABSTRACT
Acetylcholine (ACh) regulates a diverse array of physiological processes throughout the body, yet cholinergic transmission in the majority of tissues/organs remains poorly understood due primarily to the limitations of available ACh-monitoring techniques. We developed a family of G-protein-coupled receptor activation-based ACh sensors (GACh) with sensitivity, specificity, signal-to-noise ratio, kinetics and photostability suitable for monitoring ACh signals in vitro and in vivo. GACh sensors were validated with transfection, viral and/or transgenic expression in a dozen types of neuronal and non-neuronal cells prepared from several animal species. In all preparations, GACh sensors selectively responded to exogenous and/or endogenous ACh with robust fluorescence signals that were captured by epifluorescent, confocal and/or two-photon microscopy. Moreover, analysis of endogenous ACh release revealed firing pattern-dependent release and restricted volume transmission, resolving two long-standing questions about central cholinergic transmission. Thus, GACh sensors provide a user-friendly, broadly applicable toolbox for monitoring cholinergic transmission underlying diverse biological processes.
INTRODUCTION
Acetylcholine (ACh), the first identified neurotransmitter, mediates cell-to-cell communication in the central and peripheral nervous systems, as well as non-neuronal systems1–7. Cholinergic projection neurons within the mammalian brain primarily originate in three major nuclear groups1–5, including the basal forebrain nuclei (BF), and the brainstem pedunculopontine and laterodorsal tegmental nuclei. Cholinergic neurons within these groups project widely throughout the cortical and subcortical domains, consistent with their involvement in complex brain functions, including attention, perception, associative learning and sleep/awake states. Additional smaller populations of cholinergic neurons scatter throughout other brain areas (e.g., the medial habenula (MHb) and the striatum), contributing to behaviors related to motion, motivation and stress1, 3, 8. Dysregulation of central cholinergic transmission is linked to a number of brain disorders, including Alzheimer’s disease, addiction, epilepsy, Parkinson’s disease, schizophrenia and depression. In the peripheral nervous and non-nervous systems, ACh is released by both neurons and non-neuronal cells to relay fast transmission at the neuromuscular junction and to regulate functions of a variety of other tissues and organs, including the heart, liver and pancreas5–7. Dysregulation of peripheral and non-neuronal cholinergic signals is associated with multiple pathological states, including cardiovascular disease, obesity, diabetes, immune deficiency and cancer.
Despite the significance of ACh signals in many, fundamental aspects of our physiology, cholinergic transmission in the majority of tissues and organs remain poorly understood, due primarily to the limitations of tools available for the direct measurement of ACh1, 5, 9. Microdialysis, an established method for monitoring extracellular ACh10, is less frequently used in current work because of its poor spatial and temporal resolution. Patch-clamp recordings of ACh-mediated currents have excellent sensitivity and temporal resolution, but the approach is limited by the number of cells that can be recorded and the prominent desensitization of cholinergic currents3. Similarly, ACh amperometry can achieve millisecond temporal resolution, yet this method is hampered by the technically challenging electrode enzymatic coating procedure that frequently ruins its stability and repeatability11. While TANGO assay has an unparalleled sensitivity, the time-consuming transcriptional and translational amplification processes make this approach inadequate for real-time ACh measurements12. Recent technical advances in fluorescence imaging have led to the development of FRET-based sensors, which allow rapid, optical detection of released ACh, although the low sensitivity limits their application in vivo13, 14. The latest cell-based fluorescent sensors (CNiFERs) allow live imaging of ACh dynamics in behaving animals15, 16. However, the dependence of CNiFERs on invasive cell transplantation limits broad use of this method. Nevertheless, the attractive real-time imaging features of both FRET-based sensors and CNiFERs inspired us to engineer more user-friendly and broadly applicable genetically-encoded ACh sensors15, 17.
Here, we report the development of a family of genetically-encoded G-protein-coupled receptor activation-based sensors for ACh (GACh). Our GACh sensors were initially constructed by coupling a circular permutated green fluorescent protein (cpGFP) into a muscarinic acetylcholine receptor (MR), with subsequent improvements via large-scale site-directed mutagenesis and screening. The sensitivity and utility of GACh sensors were validated in cultured cells, cultured cortical neurons, tissue slices prepared from multiple brain areas and peripheral organs, the olfactory system of living Drosophila and the visual cortex in awake behaving mice in vivo. Our data indicate that GACh sensors have the sensitivity (EC50 ≈ 1 μM), specificity (≈ MRs), signal-to-noise ratio (SNR ≈ 14), kinetics (τon/off ≈ 200-800 ms) and photostability (≥ 1-4 hrs) suitable for precise and convenient real-time assays of ACh signals.
RESULTS
Development and optimization of GACh sensors
We initiated the development of a genetically-encoded optical sensor for ACh by inserting a conformationally sensitive cpGFP into all five subtypes of human muscarinic acetylcholine receptors (M1-5Rs) at their third intracellular loop (ICL3) (Fig. 1a). This site was chosen because ICL3 links the transmembrane helices 5 and 6 of muscarinic receptors and may undergo a large conformational change upon ACh binding18. To avoid creating a lengthy cpGFP-containing ICL3, that is likely to hinder the expression and surface trafficking of the receptors, and to minimize coupling of the receptors to downstream signaling, we replaced ICL3 of M1-5Rs with a shorter 24-amino acid ICL3 modified from an adrenergic β2R (Fig. S1). We then expressed all chimeras, M1-5R-β2R ICL3-cpGFP, in HEK293T cells to evaluate their response to ACh. Cells expressing the M3R chimera, M3R-β2R ICL3-cpGFP, which showed excellent membrane expression, had increased fluorescence responses (ΔF/F) (by ~30%) to bath application of ACh (Fig. 1b, red arrow head). In contrast, cells expressing the four other muscarinic receptor chimeras, all of which had poor membrane expression, exhibited no detectable ΔF/F upon ACh application (Fig. S1b). These results indicate that the M3R-derived sensor, that we called GACh1.0, can detect ACh.
To improve GACh1.0, we used site-directed mutagenesis to create a library of 723 randomized point mutations at the two- and five-amino acid linkers in the N and C termini of cpGFP in GACh1.0 (Fig. 1c and Fig. S2, see Methods for details). We then individually expressed these variants in HEK293T cells and screened for candidates with larger ΔF/F to ACh application. The screening revealed that the best variant had ΔF/F increased by ~70% compared to GACh1.0, and we named the variant GACh1.5 (Fig. 1d). In addition, this screening revealed one or multiple single-point mutations for each of the seven linker residues (total 18 hits) that produced relatively larger ΔF/F responses to ACh application (Fig. 1c and S2). We initiated the second round of site-directed mutagenesis and screening, using combinations of the top hits for mutagenesis of the two-amino acid N terminus linker residues (i.e., GG) and 2-4 top hits for the five-amino acid C terminus linker residues (Fig. 1c and S2). After screening 23 combinatorial variants, we found that the variant, with the linker sequences of GG and APSVA, had the maximally enhanced ΔF/F response to ACh application, and we named this variant GACh2.0 (Fig. S2). Further analysis showed that GACh2.0 retained excellent expression and membrane trafficking properties (Fig. 1e), and had enhanced dynamic range (by 2.5-fold) compared to GACh1.0 (Fig. 1f, g; Movie S1). Compared with the FRET based ACh sensor14, GACh2.0 had ~20-fold larger peak signal responses and ~60-fold higher signal-to-noise ratio (SNR) to 100 μM ACh application (Fig. 1h-j and Fig. S3).
Characterization of GACh sensors in cultured cells and neurons
To characterize the properties of GACh, we first performed high-speed line scan imaging of fluorescent signals in the membrane surface of GACh2.0 expressing HEK293T cells in response to agonists or antagonists delivered with a rapid local perfusion system (Fig. 2a-b). Local ACh perfusion elicited increases in fluorescence intensity in GACh2.0 expressing cells that exhibited a rapid time course, while local perfusion of tiotropium, a muscarinic antagonist19, decreased fluorescent signals in GACh2.0 expressing cells bathed in 100 μM ACh, albeit with a slower time course (Fig. 2b-c). The time-dependent changes in fluorescence intensity were fitted to single exponential functions, yielding average activation and inactivation time constants of 280±32 ms and 762±75 ms, respectively (Fig. 2b-c). These values were likely overestimated due to application delay caused by the perfusion system (~80 ms, Fig. S4).
To determine the sensor’s sensitivity to ACh, we measured the fluorescence intensity of GACh2.0 expressing HEK293T cells bathed in solutions containing varied ACh concentrations (Fig. 2d). Increasing ACh from 10 nM to 100 μM progressively increased the fluorescence intensity in GACh2.0 expressing cells. The concentration-response relationship was fitted to a Boltzmann equation with EC50 of ~0.7 μM (Fig. 2e), a value that is comparable to wild type M3Rs20. The ACh-induced fluorescent signals were completely blocked by co-application of 20 μM AF-DX 384, another muscarinic antagonist21, further indicating specificity.
Activation of muscarinic receptors stimulates intracellular arrestin and Ca2+ signaling22, 23. We compared the activation of GFP-tagged wild type M3Rs (GFP-M3Rs) and GACh2.0 expressed in HEK293T cells. Control GFP-M3R expressing cells had a significant reduction (~60%) in membrane GFP fluorescence intensity within 2 hours of ACh application, which was prevented by co-application of tiotropium (Fig. S5a), consistent with a muscarinic receptor-stimulated arrestin-dependent internalization22. By contrast, surface expression of GACh2.0 remained unchanged in the presence of 1 mM ACh for as long as 4 hours (Fig. S5a), indicating little internalization. In addition, using an arrestin TANGO assay12, we found that bath application of varied concentrations of ACh induced much smaller (~1000-fold less) reporter signals in GACh2.0 expressing cells compared to control GFP-M3R expressing cells (Fig. S5b). Together, these results suggest negligible coupling between GACh2.0 and downstream arrestin signaling events. Moreover, we examined the ACh-induced concentration-dependent Ca2+ responses in both GACh2.0 and GFP-M3R expressing cells (Fig. S6a-c). The Ca2+ responses in GACh2.0 expressing cells were ~7-fold smaller than those in GFP-M3R expressing cells (Fig. S5c-e), suggesting reduced coupling of GACh to downstream Gq protein-dependent signaling23. Collectively, these results indicate that GACh2.0 can sensitively detect ACh but minimally transduces intracellular signals.
To characterize GACh’s properties in neurons, we expressed GACh1.0 and GACh2.0 in cultured cortical neurons (Fig. 2f-j). Approximately 48 hours after transfection, GACh1.0 and GACh2.0 were distributed to throughout expressing neurons, with the majority of sensors delivered to the neurites (Fig. 2f). Bath application of 100 μM ACh enhanced the fluorescence intensity in cortical neurons expressing GACh1.0 and GACh2.0 by ~30% and ~90%, respectively (Fig. 2g; Movie S2), validating the functionality of the sensors in neurons. Varying ACh concentration in the bath solution from 1 nM to 100 μM progressively increased the fluorescence intensity in GACh2.0 expressing neurons, revealing a detectable signal at ~500 nM with an EC50 of ~2 μM (Fig. 2h).
To test the selectivity of GACh2.0 in neurons, we compared responses of GACh2.0 expressing neurons to bath application of ACh, other neurotransmitters and ACh-related bio-compounds. Bath application of ACh induced a pronounced increase in fluorescence intensity in GACh2.0 expressing neurons that was completely blocked by including additional 2 μM tiotropium in the bath solution. In contrast, bath application of nicotine, choline, glycine, serotonin, epinephrine, GABA, glutamate, dopamine, norepinephrine, histamine, and adenosine did not induce detectable change in fluorescence in GACh2.0 expressing neurons (Fig. 2i,j). Finally, we noted no alteration in membrane fluorescence intensity in GACh2.0 expressing neurons during a 30-minute bath application of 100 μM ACh (Fig. S6a-c), consistent with the minimal arrestin-dependent internalization of GACh2.0 in HEK293T cells. Together, these results support the utility of GACh as specific ACh sensors in neurons.
Validation of GACh sensors in cultured brain slices
To further test the applicability of GACh sensors, we expressed the sensors in CA1 pyramidal neurons in cultured mouse hippocampal slices. We first used a lentiviral expression system to express GACh sensors in CA1 neurons for 7-14 days to provide sufficient time for GACh expression and trafficking to distal dendrites (Fig. S7). Two-photon imaging showed that GACh1.0, GACh1.5 or GACh2.0 were expressed throughout CA1 pyramidal neurons, with significant expression at plasma membrane of somata, dendrites and spines, sites of excitatory synapses (Fig. S7). To examine whether GACh sensors can detect ACh in the cultured slice preparation, we chose a Sindbis viral expression system that allowed a more rapid (~18 hours) and robust expression of GACh sensors in CA1 neurons (Fig. S8a; see the methods). Using an epifluorescent microscope, we captured fluorescence responses in GACh expressing neurons. A brief 500-ms puff application of ACh or a muscarinic agonist, oxotremorine20, evoked fluorescence responses in CA1 neurons expressing GACh1.0, GACh1.5 or GACh2.0, whereas puff application of a nicotinic agonist, nicotine, or control bath solution ACSF, induced no responses in the same neurons (Fig. S8b-d; Movie S3), indicating that GACh sensors selectively responded to ACh or muscarinic agonists.
Because GACh2.0 produced the largest ΔF/F to ACh in the above tests, we focused our investigation mainly on this sensor in the following experiments. We found that multiple puffs induced equivalent fluorescence responses in GACh2.0 expressing CA1 neurons (Fig. S9), suggesting photostability of GACh2.0. Bath application of 1 μM atropine, a muscarinic antagonist21, but not 2,2,6,6-tetramethylpiperidin-4-yl heptanoate, a nicotinic antagonist24, completely blocked ACh-induced fluorescence responses in GACh2.0 expressing CA1 neurons (Fig. S9), again indicating a specific muscarinic effect. Importantly, we found that the resting membrane potential, input resistance, membrane time constant and average spiking frequency of expressing GACh2.0 CA1 neurons were not different from nearby control non-expressing neurons (Fig. S10a-d), suggesting no effect of GACh2.0 expression on basic membrane properties. Similarly, AMPA, NMDA or GABAergic responses, or paired pulse facilitation of AMPA responses in GACh2.0 expressing CA1 neurons remained unchanged (Fig. S10e-h), indicating no alteration of synaptic transmission by GACh2.0 expression. Collectively, these results are consistent with the finding that GACh2.0 is a selective, photostable ACh sensor.
To compare the approaches of GACh2.0 imaging and patch-clamp recordings, we simultaneously made dual whole-cell recordings and fluorescence imaging from a pair of neighboring GACh2.0 expressing and control non-expressing CA3 pyramidal neurons in cultured mouse hippocampal slices (Fig. 3a); CA3 pyramidal neurons were chosen because they express relatively more endogenous muscarinic and nicotinic receptors and respond robustly to cholinergic activation25. A brief 500-ms ACh puff evoked a brief, large inward current followed by a prolonged, small inward current in both GACh2.0 expressing and control non-expressing CA3 neurons, presumably representing the responses of endogenous nicotinic and muscarinic receptors, respectively3 (Fig. 3b-d). A large concurrent fluorescent signal was observed only in GACh2.0 expressing neurons, but not in control non-expressing CA3 neurons (Fig. 3b-d). The latencies of cholinergic current responses and fluorescence signals were the same in GACh2.0 expressing neurons (Fig. 3b,e), indicating that GACh2.0 detected ACh as fast as endogenous cholinergic receptors. The SNR of GACh2.0 fluorescence responses (~14) was smaller than that of the fast nicotinic-like cholinergic current responses (~35), but larger than that of the slow muscarinic-like cholinergic current responses (~8) (Fig. 3b,f), indicating that GACh2.0 had a relatively comparable resolving power compared to the patch-clamp recordings in monitoring cholinergic signals. Notably, the second ACh puff evoked the same fluorescence responses, but smaller cholinergic currents (reduced by ~40%) in GACh2.0 expressing CA3 neurons compared to the first ACh puff (Fig. 3b,g,h), due presumably to desensitization of endogenous receptors3. There was no difference in the amplitude, latency or SNR of cholinergic currents in GACh2.0 expressing and control non-expressing CA3 neurons (Fig. 3b-f), confirming that expression of GACh2.0 had little non-specific effect on CA3 neurons. These results suggest that GACh2.0 is suitable for repeatedly and faithfully monitoring ACh signals.
Applications of GACh sensors in acute brain slices
To examine whether GACh2.0 detects ACh when expressed in the other brain areas and other animal species, we made in vivo Sindbis viral expression of GACh2.0 in a number of preparations, including layer 2 (L2) stellate neurons and L1 interneurons in the medial entorhinal cortex (MEC), L5 pyramidal neurons in the barrel cortex of mice, GABAergic thalamic reticular neurons and glutamatergic thalamocortical neurons in the ventral basal nucleus of rats. Approximately 18 hours after in vivo expression, we prepared acute brain slices containing these brain regions and measured ΔF/F to a brief puff application of ACh (Fig. S11a). Puff applications of ACh and oxotremorine, but not of nicotine or bath solution ACSF, evoked fluorescence increases in GACh2.0 expressing entorhinal L2 stellate neurons and L1 interneurons, barrel cortical L5 pyramidal neurons, thalamic reticular neurons and thalamocortical neurons (Fig. S11b-c). Collectively, these results suggest that GACh2.0 can selectively detect ACh in various brain areas of both mice and rats.
To test whether GACh2.0 can report endogenously released ACh, we used the same in vivo viral expression and ex vivo acute mouse slice preparation to measure ΔF/F responses of GACh2.0 expressing entorhinal L2 stellate neurons to electrical stimulation of MEC L1 (Fig. 4a), a layer that is densely innervated by cholinergic fibers originating from BF26, 27. Twenty pulses at 2-Hz evoked robust fluorescence responses in GACh2.0 expressing neurons (Fig. 4b,c; Movie S4). Repeated electric stimuli delivered every 8 minutes induced the same ΔF/F responses in GACh2.0 expressing neurons (Fig. 4d-e), indicating that GACh2.0 is suitable for monitoring endogenously released ACh signals over relatively long durations. Systematically varying the stimulation frequency revealed that low frequency stimuli (0.5–2 Hz) evoked large, plateau-like fluorescence responses, intermediate frequency stimuli (4–12 Hz) elicited more rapidly rising but briefer fluorescence responses, while high frequency stimuli (≥32 Hz) induced little fluorescence responses in GACh2.0 expressing neurons (Fig. 4f-h). Previous studies have shown that BF cholinergic neurons in behaving animals prefer low frequency (~0.5–2 Hz) tonic firing and theta rhythmic (~4—12 Hz) phasic firing28, 29. Our results suggest that these two preferred firing patterns generate distinct sustained and transient ACh release events, which are observed when animals perform different behavioral tasks9. Further altering the number of pulses delivered in 2-Hz electric stimuli showed that single electric pulses elicited detectable ΔF/F responses, whereas multiple pulses induced enhanced ΔF/F responses in GACh2.0 expressing neurons (Fig. 4i-k), suggesting that the amount of released ACh may scale the number of presynaptic action potentials.
To verify the cholinergic nature of electrically evoked ΔF/F responses, we included atropine in the bath solution. Unexpectedly, bath application of 1 μM atropine, which was sufficient to block ACh puff-evoked ΔF/F responses (Fig. S9), failed to block L1 stimulation-evoked ΔF/F responses, and at times, slightly enhanced ΔF/F responses in GACh2.0 expressing neurons (Fig. S12b). Although the atropine-induced enhancement of ΔF/F responses is surprising, the finding is consistent with previous reports of the atropine-potentiated ACh release attributed to a predominant presynaptic muscarinic autoreceptor inhibition mechanism30–32. Since presynaptic muscarinic receptors are primarily M2Rs and/or M4Rs21, 33, 34, we examined whether a similar presynaptic inhibition mechanism may exist in cholinergic transmission in MEC with (5R,6R)6-(3-propylthio-1,2,5-thiadiazol-4-yl)-1-azabicyclooctane (PTAC), which is both an agonist of M2,4Rs and an antagonist of M1,3,5Rs35. Bath application of 20 μM PTAC, which is sufficient to activate M2,4Rs and inhibit M3R-like GACh2.0, blocked the electrically evoked ΔF/F responses in GACh2.0 expressing stellate neurons (Fig. S12c). Together, these pharmacological findings are consistent with the view that a presynaptic M2,4R-mediated inhibition mechanism regulates ACh release in MEC.
To determine whether GACh sensors can report other modes of endogenous ACh release, we studied glutamatergic neurons in the medial habenula (MHb), which release ACh as a co-neurotransmitter during high frequency firing8, 36. To image the regulation of habenula cholinergic transmission, we made AAV viral expression of GACh2.0 in the interpeduncular nucleus (IPN), the major target of MHb cholinergic neurons. Two weeks after GACh2.0 expression, we prepared acute brain slices containing the source nucleus of MHb, the target IPN and the projections between the two areas carried by the fasciculus retroflexus (MHb-fr-IPN) (Fig. S13a,b). Two-photon imaging showed that brief 1-, 10-, 20- or 50-Hz 5-second electric stimuli evoked little change in fluorescence, whereas application of 100-Hz stimuli elicited small ΔF/F responses in GACh2.0 expressing IPN neurons (Fig. S13e,g). We have recently reported that GABA potentiates the optically evoked habenula cholinergic currents in IPN neurons via a presynaptic GABAbR-mediated heteroreceptor enhancement mechanism29. Consistent with those findings, GABA and baclofen, a GABAb receptor agonist, enhanced ΔF/F responses in GACh2.0 expressing neurons by 6 fold, whereas saclofen, a GABAb receptor antagonist, reversed the potentiative effects. Moreover, tiotropium completely abolished the potentiated ΔF/F responses, while donepezil, an acetylcholinesterase inhibitor37, prolonged the potentiated ΔF/F responses in GACh2.0 expressing neurons (Fig. S13d, f, h-j). These results confirm the presynaptic heteroreceptor enhancement mechanism in habenula cholinergic transmission with a GACh2.0-dependent optical approach. We next examined the effects of GABA on frequency-dependent ACh release in IPN. In the presence of 2 μM baclofen, 10-Hz electric stimuli evoked small ΔF/F responses in GACh2.0 expressing IPN neurons, which were progressively enhanced with increasing frequency from 20–100 Hz. These findings support the notion that in the presence of GABA in IPN, habenula neurons, which fire action potentials at frequencies up to 10–25 Hz in vivo38, can enhance cholinergic transmission to interpeduncular neurons, thought to be critical for fear control36. Together, the data collected from MEC and MHb-fr-IPN indicate that different firing patterns can create distinct ACh release modes in the central cholinergic system.
In addition to the control of ACh release, another unresolved fundamental question about cholinergic signaling is the extent of volume transmission. During the investigation of firing pattern-controlled ACh release, we noted that at times, the electrically evoked fluorescence responses exhibited obvious spatial heterogeneity across subcellular areas of GACh2.0 expressing L2 stellate neurons. Analysis of the minimal electric stimulation-evoked subcellular fluorescence responses in GACh2.0 expressing neurons revealed one or a few hot spots with largest ΔF/F responses, whereas other areas had smaller or undetectable changes in ΔF/F (Fig. 5), suggesting spatially restricted release and clearance of ACh. Plotting the size of ΔF/F responses against the distance from the spots with maximal ΔF/F responses yielded an estimated volume transmission spread length constant of 9.0 μm for cholinergic transmission (Fig. 5e).
Because the estimated cholinergic volume transmission size in MEC is rather small, we sought to independently verify our finding. Using the same in vivo Sindbis expression and ex vivo acute mouse slice preparation, we examined the minimal electric stimulation-evoked local fluorescence responses along the somatodendritic axis of GACh2.0 expressing CA1 neurons. Consistent with previous studies25, we found that electric stimuli of the stratum oriens and stratum pyramidale were most likely to elicit cholinergic responses in GACh2.0 expressing CA1 neurons. The largest fluorescence responses were typically observed at one or at most a few hot spots in somata of GACh2.0 expressing neurons, whereas fluorescence responses at other somatic and dendritic areas of the same neurons were much smaller or undetectable (Fig. S14). Plotting the size of ΔF/F responses against the distance from the maximal ΔF/F responses gave an estimated volume transmission spread length constant of 15.6 μm for the cholinergic transmission in CA1 neurons (Fig. S14d), confirming that the central cholinergic volume transmission is spatially restricted.
To test whether GACh sensors may be employed together with other optical techniques, we examined the feasibility of an all-optical approach, namely optogenetic activation as well as optical report of cholinergic transmission simultaneously. We again used the in vivo viral expression and ex vivo acute mouse slice preparation to examine fluorescence responses in neurons of MEC, one of the brain areas that receives the densest cholinergic inputs26, 27. Specifically, in ChAT-Cre mice, we first injected AAV virus into BF of ChAT-Cre mice to express DIO-oChIEF-tdTomato and allowed three weeks for the optogenetic probe to be expressed and transported to the terminal fields within MEC39. We then injected Sindbis virus to express GACh2.0 in MEC layer 2 stellate neurons MEC and allowed 18 hours for the sensor to be expressed before preparing acute entorhinal cortical slices. During the experiments, we employed single-photon laser pulses to optogenetically stimulate oChIEF-expressing cholinergic fibers in MEC, and simultaneously used two-photon laser scanning, which is insufficient to activate oChIEF-expressing fibers40, to image fluorescence responses in GACh2.0 expressing stellate neurons in MEC (Fig. S15a). Twenty 5-ms 473-nm single-photon laser pulses (at 1 Hz) elicited consistent fluorescence responses in GACh2.0 expressing neurons, which were largely blocked by bath application of 20 nM PTAC (Fig. S15b-c). These results establish the feasibility of all-optical activation and measurement of ACh release.
Applications of GACh sensors in non-neuronal tissues
Given the role of peripheral and non-neuronal cholinergic signals in diverse physiological actions in a large variety of non-neuronal cells5–7, we explored the utility of GACh2.0 ACh reporting in some non-neuronal cells and tissues. ACh released from parasympathetic nerve terminals acts on the pancreas to trigger insulin secretion in response to food uptake41. We made Sindbis viral expression of GACh2.0 in the mouse pancreas in vivo for ~18 hrs, and then imaged fluorescence responses of GACh2.0 expressing cells in acutely prepared pancreas tissue slices (Fig. 6a). Single electric shocks of local parasympathetic cholinergic fibers evoked evident fluorescence responses in GACh2.0 expressing pancreatic cells (Fig. 6b,c; Movie S5). Furthermore, increasing the number of stimulation pulses delivered at 2 Hz linearly increased the amplitude of ΔF/F responses (Fig. 6d,e). As with central cholinergic transmission in MEC, bath application of 1 μM atropine failed to block, whereas 20 μM PTAC blocked ΔF/F responses in GACh2.0 expressing pancreatic cells (Fig. 6f,g), consistent with the engagement of a possible presynaptic inhibition mechanism. In addition, we tested the applicability of GACh2.0 in the mouse adrenal gland using a similar in vivo viral expression and acute tissue slice imaging approach (Fig. S16a). Similar to pancreastic cells, single local electric stimuli applied to activate cholinergic fibers that innervate the adrenal gland42 evoked detectable ΔF/F responses in GACh2.0 expressing adrenal cells (Fig. S16b; Movie S6). Increasing the number of stimulation pulses increased the amplitude of ΔF/F responses that plateaued after 10 pulses (Fig. S16c). Again, 1 μM of atropine failed to block, while 20 μM PTAC blocked ΔF/F responses in GACh2.0 expressing adrenal cells (Fig. S16d), again in agreement with a possible presynaptic inhibition mechanism. Together, these results suggest that GACh2.0 can monitor ACh in non-neuronal cells.
Applications of GACh sensors in transgenic Drosophila in vivo
Next, we tested whether GACh sensors could monitor functional cholinergic transmission in the intact brain of Drosophila in vivo. We performed two-photon imaging of odor-induced synaptic responses in the antennal lobe of Drosophila, which consists of powerful cholinergic synaptic inputs from olfactory receptor neurons to principal projection neurons43. We created UAS-GACh1.0 and -GACh2.0 transgenic flies, and then crossed them with a GH146-Gal4 driver line44 to selectively express GACh1.0 and GACh2.0 in antennal lobe projection neurons. In head-fixed living GACh transgenic flies, application of the odorant isoamyl acetate induced region-specific and dose-dependent ΔF/F responses in the DM2 glomerulus, that was not observed in the DA1 glomerulus (Fig. 7a-e; cf.45, 46). The control application of odor solvent, mineral oil alone did not evoke significant ΔF/F changes in GACh transgenic flies (Fig. 7b-c). As expected, the odor-evoked changes in ΔF/F in GACh2.0 transgenic flies were ~2-fold larger than those in GACh1.0 transgenic flies (Fig. 7e). Using the spectrum non-overlapping red Ca2+ indicator RGECO47, we re-examined the odor-induced effects in both control RGECO and GACh1.0/2.0::RGECO transgenic flies. We found the isoamyl acetate-induced Ca2+ transients in similar glomerular areas in control RGECO, GACh1.0::RGECO and GACh2.0::RGECO transgenic flies (Fig. S17), confirming the results obtained with GACh sensors. Further analysis showed that the isoamyl acetate-evoked Ca2+ transients in the glomerulus DM2 were indistinguishable between control RGECO, GACh1.0::RGECO and GACh2.0::RGECO transgenic flies (Fig. S17), suggesting that expression of GACh sensors had little effect on the endogenous receptor-mediated Gq-coupled Ca2+ signaling. Taken together, the results indicate that GACh can effectively monitor the dynamics of physiological ACh release in functional neuronal circuits in Drosophila in vivo.
Application of GACh sensors in mouse visual cortex in vivo
Finally, we tested whether GACh sensors were able to detect the endogenous ACh release in rodent brain in vivo. We used a visual attention model in a 2-photon setting of awake mice48. Specifically, GACh sensors were sparsely expressed by injection of Sindbis virus in visual cortex after behavioral habituation of mice, and in vivo two photon imaging experiments were conducted 16 h later with visual stimuli delivered by a monitor in front of the mice (Fig. 8a; see methods; cf.49). To monitor potential ACh release in the visual cortex, white filled circles expanding on the screen (10 s) were given as stimuli designed to engage visual attention, followed by 50 s darkness (Fig. 8b). Interestingly, we observed a subset of GACh2.0 expression neurons (ROI#1) repeatedly responded to the expanding circle with increase of fluorescence signals, consistent with ACh release upon attentional states50, 51, while other subset of neurons (ROI#2) remained quiescent in fluorescence (Fig. 8b,c), which may reflect a functional heterogeneity and complex cholinergic projection in the visual cortex. Analysis of multiple responding cells in different animals revealed a time-lock and statistically significant fluorescence response to the visual stimulation (Fig. 8d). Taken together, the GACh sensors were sensitive enough to report the attentional like visual stimuli evoked ACh release in awake behaving mice, which opens the door to study ACh function in many other brain regions in rodents in vivo.
DISCUSSION
In this study, we have developed and validated a family of genetically-encoded fluorescent ACh probes, or GACh sensors. GACh sensors have the sensitivity, ligand specificity, SNR, kinetics and photostability, the properties that are suitable for monitoring cholinergic signals in diverse tissue preparations in vitro, ex vivo and in vivo. Using the new GACh sensors, we revealed a few key features of cholinergic transmission, including firing pattern-dependent release and spatially restricted volume transmission. We also demonstrated the feasibility of utilizing GACh sensors to visualize endogenous ACh transmission in both neuronal and non-neuronal tissues, providing the possibility for deciphering cholinergic mechanisms in diverse physiological and pathological conditions.
The genetically-encoded GACh sensors represent a new toolbox suitable for a wide-range of preparations. We show here that the sensors can be expressed in a dozen distinct types of cells (i.e., HEK293T cells, cultured cortical neurons, hippocampal CA1 and CA3 pyramidal neurons, entorhinal cortical L1 interneurons and L2 stellate neurons, barrel cortical L5 pyramidal neurons, interpeduncular neurons, visual cortex, and pancreas and adrenal cells in the mouse, thalamocortical neurons and thalamic reticular neurons in the rat, and olfactory projection neurons in the fly) using transfection, viral expression (AAV, Sindbis and lenti-virus) or transgenic techniques. In all the preparations, GACh sensors effectively detected exogenous and/or endogenous ACh with high specificity, and the ACh-evoked optical signals could be captured with epi-fluorescent, confocal and/or two-photon microscopy in vitro and in vivo. Overall, GACh sensors have EC50 equivalent to ~1 μM, ΔF/F sensitivity of up to ~100%, activation/inactivation kinetics within sub-second, and photostability capability to repeatedly report ACh for hours. We note that GACh sensors have a weak coupling to the downstream G protein intracellular signaling in cultured cells, yet this persistent weak coupling has no detectable effect on basic membrane properties, synaptic properties and cholinergic transmission in rodent neurons in vitro, or the sensory input-evoked cholinergic responses in fly and attention induced ACh release in mice visual cortex in vivo. We expect additional site-directed mutagenesis and screening to generate successor GACh sensors with even better performance (e.g., improved sensitivity and further reduced intracellular signaling generation) in the near future.
With the newly developed GACh sensors, we have investigated a few fundamental questions of cholinergic transmission. Central cholinergic neurons exhibit multiple distinct action potential firing patterns28, 29, 38, yet the functional significance of these firing patterns remains elusive. Here we report that BF cholinergic neurons use low frequency 0.5-2 Hz tonic firing to generate large plateau-like postsynaptic ACh signals, and 8-12 Hz theta rhythmic phasic firing to elicit small transient postsynaptic ACh signals. High-frequency activation of cholinergic fibers seems to be more effective in recruiting presynaptic auto-receptor inhibition mechanism to suppress ACh release32. As such, the low frequency firing-induced releases may be less suppressed and thereby generate plateau-like ACh signals, whereas the high frequency firing-induced release seems to yield more transient ACh signals. In addition, habenula neurons can fire high frequency action potentials of up to 10-25 Hz35. The firing triggers co-release of ACh with its primary neurotransmitter glutamate when a presynaptic GABAb-R-mediated enhancement mechanism is engaged. Although the detailed aspects of this regulation remain to be further dissected, our data are consistent with the view that presynaptic regulatory mechanisms (i.e., autoreceptor inhibition and heteroreceptor enhancement) may play key roles in governing release modes in central cholinergic transmission.
In addition, a long-standing question of central cholinergic transmission concerns the extent of volume transmission; some investigators propose that ACh acts globally affecting a large number of neurons, whereas others consider cholinergic volume transmission to be be more restricted1–3, 9. The new ACh sensors have allowed us to directly visualize the spread of released ACh in the hippocampus and MEC, and estimate cholinergic volume transmission spread length constant of ~10-15 μm. Because the minimal electric stimulation can still activate multiple ACh release sites, the calculated volume transmission spread length constants may be overestimated. Nevertheless, these estimations provide the first suggestion that central cholinergic volume transmission may have single-cell or subcellular specificity. Together, fine firing frequency-controlled release and spatially restricted volume transmission advance our fundamental understanding of the control and precision of cholinergic signaling.
Our ability to visualize ACh signals in rodent models ex vivo and in vivo aided by the newly developed ACh sensors should help to advance our understanding of the pathogenesis of various diseases. For example, cholinergic signals are essential for high-level cognitive functions, including learning and memory, and dysregulation of cholinergic transmission is observed in many neurological disorders, including Alzheimer’s disease. However, the cholinergic hypothesis-based acetylcholinesterase inhibitor treatment, the only available therapy for Alzheimer’s disease33, has limited efficacy and thus is far from ideal37, 52. Further understanding the mechanisms of central cholinergic transmission in physiological and pathological conditions is central to development of effective therapeutic strategies for Alzheimer’s disease. Moreover, defective cholinergic signals have been implicated in the pathophysiology and treatment of a number of other non-neurological diseases5–7. For instance, ACh modulates insulin secretion from the pancreatic islets of Langerhans41 and the activity of M3Rs is essential for glucose tolerance and insulin release34, 53. Moreover, ACh controls release of hormones in the adrenal critical for regulation of stress and blood pressure42. Last but not least, cholinergic signals seem to control the progress of inflammation54 and tumorigenesis55, 56. We show here that GACh sensors are effective in monitoring cholinergic transmission in non-neuronal cells as well, including the pancreas and the adrenal, endorsing the use of this new toolbox to unravel the cholinergic mechanisms underlying these medical conditions.
METHODS
Animal preparation
Male and female Sprague Dawley rats, and wild type and ChAT-Cre transgenic C57BL/6 mice were used to prepare cultured neurons, cultured hippocampal slices, acute brain slices, acute pancreas and adrenal slices in this study. Animals were maintained in the animal facilities at the Peking University, the National Institute of Biological Sciences, Beijing, China, University of Southern California, Stony Brook University or the University of Virginia, and family or pair housed in the temperature-controlled animal room with 12-h/12-h light/dark cycle. All procedures for animal surgery and maintenance were performed following protocols approved by the Animal Care & Use Committee of the Peking University, the National Institute of Biological Sciences, Beijing, China, University of Southern California, Stony Brook University or the University of Virginia and in accordance with US National Institutes of Health guidelines.
Preparations of cultured cells, cultured neurons and cultured slices
HEK293T were cultured in DMEM (Gibco, MA) with 5% FBS (North TZ-Biotech Develop Co., Ltd, Beijing, China) at 37 °C, 5% CO2, and passed to poly-D-lysine (Sigma-Aldrich, MO) coated 12-mm glass coverslips in 24 well plates. Rat cortical neurons were prepared from postnatal 1-day old (P1) Sprague-Dawley rats as previously described45. The rat brains were dissected and digested by 0.25% Trypsin-EDTA (Gibco), and placed on to poly-D-lysine coated coverslips with density of 0.5-1×106 cells/ml.
Cultured slices were prepared from P6-7 rats or mice following our previous studies57, 58. In brief, the hippocampi were dissected out in ice-cold HEPES-buffered Hanks’ solution (pH 7.35) under sterile conditions, sectioned into 400 μm slices on a tissue chopper, and explanted onto a Millicell-CM membrane (0.4-μm pore size; Millipore, MA). The membranes were then placed in 750 μl of MEM culture medium, contained (in mM): HEPES 30, heat-inactivated horse serum 20%, glutamine 1.4, D-glucose 16.25, NaHCO3 5, CaCl2 1, MgSO4 2, insulin 1 mg/ml, ascorbic acid 0.012% at pH 7.28 and osmolarity 320. Cultured slices were maintained at 35°C, in a humidified incubator (ambient air enriched with 5% CO2).
Preparations of acute tissue slices
Acute thalamic, barrel cortical, entorhinal cortical, hippocampal and MHb-fr-IPN brain slices, pancreas and adrenal tissues slices were prepared from P25-60 animals deeply anesthetized by xylazine-ketamine or pentobarbital (100 mg/kg) as described in our previous reports36, 58. The animals were decapitated and the brain block containing the thalamus, barrel cortex, MEC and/or hippocampus, the pancreas, or the adrenal was quickly removed and placed into cold (0-4°C) oxygenated physiological solution containing (in mM): 125 NaCl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 1 MgCl2, 25 dextrose, and 2 CaCl2, pH 7.4. The brain blocks were directly sectioned into 400-μm-thick brain slices using a DSK microslicer (Ted Pella Inc.) or a VT1200 vibratome (Leica, Germany), while the pancreas and adrenal were first embedded in low–melting temperature agar (2.5% in BBS) and then sectioned into 400-μm-thick tissue slices59. The tissue slices were kept at 37.0±0.5°C in oxygenated physiological solution for ~0.5-1 hr before imaging. During the recording and/or imaging the slices were submerged in a chamber and stabilized with a fine nylon net attached to a platinum ring. The recording chamber was perfused with oxygenated physiological solution. The half-time for the bath solution exchange was ~6 s, and the temperature of the bath solution was maintained at 34.0 ± 0.5 °C. All antagonists were bath applied.
Molecular biology
Molecular cloning was typically carried out using the Gibson assembly60 with ~30-overlapping base primers and the Phusion DNA polymerase (New England Biolabs, MA), and verified by Sanger sequencing using in an in-house facility (sequencing platform in the School of Life Sciences of the Peking University). The chimeric GACh constructs were generated by subcloning full-length human GPCR cDNAs (hORFeome database 8.1, the Dana-Farber Cancer Institute Center for Cancer Systems Biology) into the pDisplay vector (Invitrogen, MA), with an IgK leader sequence inserted before the coding region and a stop codon inserted before the PDGFR transmembrane domain. The ICL3 of M1-5Rs, defined according to the UNIPROT database (Fig. S1), was replaced by a shorter cpGFP-inserted ICL3 from a β2 adrenergic receptor. The site-directed mutagenesis of the sequences of the two- and five-amino acid linkers in the N and C termini of cpGFP was made using primers containing various lengths of tr-inucleotides NNB (20 possible amino acids, Sangon Biotech, Shanghai, China). The applicable GACh sensors were then subcloned into the Sindbis viral vector, the lentiviral vector or the AAV package vector under the human synapsin promoter to ensure the neuronal expression. To create the transgenic Drosophila, fragments of GACh sensors including the IgK leader sequence were cloned into the pUAST vector, and subsequently injected into Drosophila embryo following a standard protocol (Fungene biotechnology, Beijing). To report the receptor endocytosis, super ecliptic pHluorin43 was cloned to the N terminus of M3R, with a three amino acid linker (GGA) to ensure correct protein folding and trafficking.
Expression of GACh sensors and other recombinant proteins
HEK293T cells were typically transfected using the PEI method (with a typical ratio of 1 μg DNA:4 μg PEI), media replaced 4-6 h later, and imaged 24 h later. Cultured neurons were transfected after 7-9 days in vitro using the calcium phosphate transfection method and experiments were performed 48 h after transfection. Neurons in hippocampal cultured slices were infected after 8–18 days in vitro with lentivirus or Sindbis virus, and then incubated on culture media and 5% CO2 before experiments. For in vivo expression, P28-84 animals were initially anesthetized by an intraperitoneal injection of 2,2,2-Tribromoethanol (Avetin, 500 mg/kg) or ketamine and xylazine (10 and 2 mg/kg, respectively), and then placed in a stereotaxic frame. In some of the animals, AAV of GACh sensors (with a titer of >1012/ml) was injected into IPN with a microsyringe pump (Nanoliter 2000 Injector, WPI) using the previously described coordinates (AP: −3.13 mm from Bregma; DV: −4.95 mm; ML: 1.33 mm with 15° angle towards the midline)8, 36. Two weeks after the viral injection, acute MHb-fr-IPN brain slices were prepared from these for experiments. In other animals, a glass pipette was used to penetrate into the thalamic ventrobasal nucleus, thalamic reticular nucleus, the barrel cortex and MEC according to stereotaxic coordinates, or the dissected pancreas and adrenal, to deliver ~50 nl of viral solution by pressure injection to infect neurons, or pancreas and adrenal cells with GACh sensors. Acute brain or tissue slices of 400 μm thick were then prepared from these animals ~18 h later to carry out experiments. In the rest animals, or ChAT-Cre transgenic mice, AAV of DIO-oChIEF-tdTomato was first injected into BR according to the previously described coordinates39, and three weeks later, Sindbis virus of GACh sensors was injected into the MEC for ~18 h before preparing acute brain slices for experiments.
Electrophysiology
Simultaneous dual whole-cell recordings were obtained from two nearby infected and non-infected hippocampal CA1 pyramidal neurons under visual guidance using fluorescence and transmitted light illumination40, 58. The patch recording pipettes (4-7 MΩ) were filled with intracellular solution containing 115 mM cesium methanesulfonate, 20 mM CsCl, 10 mM HEPES, 2.5 mM MgCl2, 4 mM Na2ATP, 0.4 mM Na3GTP, 10 mM sodium phosphocreatine, 0.6 mM EGTA, and 0.1 mM spermine and 0.5% biocytin (pH 7.25) for voltage-clamp recordings, or containing 120 mM potassium gluconate, 4 mM KCl, 10 mM HEPES, 4 mM MgATP, 0.3 mM Na3GTP, 10 mM sodium phosphocreatine and 0.5% biocytin (pH 7.25) for current-clamp recordings. Bath solution (29±1.5°C) contained (in mM): NaCl 119, KCl 2.5, CaCl2 4, MgCl2 4, NaHCO3 26, NaH2PO4 1, glucose 11, picrotoxin (PTX) 0.1, bicuculline 0.01, and 2-chloroadenosine 0.002, at pH 7.4 and gassed with 5% CO2/95% O2. PTX was excluded when GABA responses were examined. Whole-cell recordings were made with up to two Axoclamp 2B or Axopatch-200B patch clamp amplifiers (Molecular Devices, Sunnyvale, CA). Junction potentials were not corrected. Synaptic responses were evoked by bipolar electrodes with single voltage pulses (200 μs, up to 20 V). Synaptic AMPA and NMDA responses at −60 mV and +40 mV or GABA responses at 0 mV were averaged over 90 trials. To minimize the effect from AMPA responses, the peak NMDA responses at +40 mV were measured after digital subtraction of estimated AMPA responses at +40 mV. Cholinergic fibers in tissue slices were stimulated with a bipolar electrode placed ~50–200 μm from imaged cells with single or a train of voltage pulses (500 μs, up to 50 V) to evoke ACh release. Optogenetic activation of oChIEF-expressing cholinergic fibers was made with a train of 5-ms light pulses delivered at 1 Hz from a 470-nm blue M470F1 LED (Thorlabs, NJ). The blue light of the LED was fiber-coupled to an Ø200 μm fiber optic cannula positioned ~250 μm away from imaged neurons. The light power out of the cannula was set at 2 mW.
Fluorescence imaging of cultured cells and neurons
HEK293T cells transfected with the muscarinic receptor-based chimeric constructs were imaged with a TECAN Safire2 fluorescence plate reader (TECAN, Männedorf, Switzerland; excitation, 480 nm; emission, 520 nm). During the measurement, the culture media was replaced with 100 μl Tyrode solution containing ACh at varied concentrations from 0-100 μM. The ΔF/F of each construct was obtained by averaging the ACh-induced fluorescence responses of 2-6 transfected wells (>100 cells/well) after digitally subtracting that of neighboring control non-transfected wells.
HEK293T cells and cultured neurons were perfused with standard extracellular Tyrode solution containing (in mM): 150 NaCl, 4 KCl, 2 MgCl2, 2 CaCl2, 10 HEPES and 10 Glucose, with pH of 7.4, in an imaging chamber during imaging. Agonist acetylcholine (Solarbio, Beijing, China), tiotropium bromide (Dexinjia Bio & Tech Co., Ltd, Jinan, China), isoamyl acetate and AF-DX 384 (Sigma-Aldrich) were delivered with a custom-made perfusion system and/or bath applied. The chamber was washed with Tyrode solution between applications and cleaned with 75% ethanol between experiments. HEK293T cells were imaged using an IX81 inverted microscope (Olympus, Tokyo, Japan) with a 40x/1.35 NA oil objective, a 475/28 excitation filter for cpGFP excitation, a 506LP dichroic mirror and a 515LP emission filter for imaging collection. The data were acquired with a Zyla sCMOS DG-152V-C1e-FI camera (Andor Technology, UK) controlled by μManager. Cultured neurons were imaged using an inverted Nikon Ti-E A1 confocal microscope (Nikon, Tokyo, Japan) with a 40x/1.35 NA oil objective and a 488 nm laser illumination.
Fluorescence imaging of cells in cultured and acute slice preparations
Wide field epifluorescence imaging was performed using Hamamatsu ORCA FLASH4.0 camera (Hamamatsu Photonics, Japan) and GACh expressing cells in cultured hippocampal slices and acutely prepared brain slices are excited by a 460-nm ultrahigh-power low-noise LED (Prizmatix, Givat-Shmuel, Israel). The frame rate of FLASH4.0 camera was set to 10 Hz. To synchronize image capture with drug perfusion, electrical stimulation, and/or electrophysiological recording, the camera was set to external trigger mode and triggered by a custom-written IGOR Pro 6 program (WaveMetrics, Lake Oswego, OR). Agonists or antagonists, including acetylcholine and atropine (Sigma-Aldrich), and nicotine, oxotremorine M, TAPC and TMPH (Tocris Bioscience, Bristol, UK), were either bath applied or puff applied with a glass pipette positioned above the imaged neurons by 500-ms pressure pulses.
Two-photon imaging was performed using a custom-built microscope or an Olympus FV1000 microscope (for IPN experiments; Olympus, Japan). The parameters of frame scan were typically set at a size of 200 × 200 pixels and a speed of 1-2.5 frame/s. The fluorescence of oChIEF-tdTomato and GACh2.0 was excited by a femtosecond Ti:Sapphire laser (Chameleon Ultra II, Coherent) at a wavelength of 950 nm. Changes in fluorescence were quantified as increases in fluorescence from baseline divided by resting fluorescence (ΔF/F0) and averaged for ~10 trials. To quantify surface expression of GACh sensors, lentiviral expression of GACh1.0, GACh1.5 or GACh2.0 was made in the CA1 region of organotypic hippocampal cultured slices. About ~1-2 weeks after expression, GACh expressing CA1 pyramidal neurons were patch-clamp recorded and loaded with 5 μM Alexa Fluor 594 (Life Technologies) for ~10 minutes, and two-photon images were then taken at different compartments along the apical dendrites. The multiple patch-clamp recordings, optogenetics, epifluorescence and two-photon imaging were typically operated by a single custom-written IGOR Pro 6 program (WaveMetrics, Lake Oswego, OR)40.
Immunocytochemistry
Mice infected with GACh sensors were deeply anesthetized with pentobarbital (400 mg/kg; i.p.), and transcardially perfused first with cold normal saline and then 4% paraformaldehyde in 0.1 M PBS. Brain blocks were post-fixed for ⩾4 hr, cryoprotected in 30% sucrose for ⩾24 h, then embedded in tissue freezing medium and sectioned into 50-μm-thick coronal sections with a freezing Leica CM 1900 microtome (Leica, Germany). To label cholinergic terminals from MHb and GACh expressing neurons in IPN, tissue sections were rinsed and immunoreacted with Goat ChAT antibody (1:500, Millipore, ab144p) and Rabbit GFP antibody (1:500, Abcam, #ab6556), and then labeled with goat-anti-rabbit second antibody conjugated Alexa 488 and donkey-anti-goat second antibody conjugated Alexa 555 after extensive washing. The immunolabeled tissue sections were imaged with a confocal microscope.
To recover the morphology of recorded neurons, the slices were fixed by immersion in 3% acrolein/4% paraformaldehyde in 0.1 M PBS at 4°C for 24 hrs after in vitro patch-clamp recordings with internal solution containing additional 1% biocytin, and then processed with the avidin-biotin-peroxidase method to reveal cell morphology. The morphologically recovered cells were examined and reconstructed with the aid of a microscope equipped with a computerized reconstruction system Neurolucida (MicroBrightField, Colchester, VT).
Fluorescence imaging of transgenic Drosophila
Transgenic Drosophila lines with strong GACh expression levels and robust odor responses were chosen after crossing UAS-GACh1.0 and -GACh2.0 transgenic flies with a GH146-Gal4 driver line44. They were reared at room temperature for 8~12 days on standard medium after eclosion before experiments. For imaging experiments, the mounting of the live flies was essentially similar as described before61. Live flies were mounted to a small dish, with their rectangular patch of cuticle between the eyes, excessive fat bodies and air sacs surrounding the antennal lobe removed, and the pair of muscles underneath the proboscis cut to reduce the brain movement. The exposed brains were perfused (at 0.5 ml/min) with adult-like hemolymph (ALH) containing (in mM): 108 NaCl, 5 KCl, 5 HEPES, 5 Trehalose, 5 sucrose, 26 NaHCO3, 1 NaH2PO4, 2 CaCl2 and 1~2 MgCl2, with osmolarity 275 mOsm. 5% CO2/ 95% O2 were continuously bubbled to maintain ALH at pH 7.3. Isoamyl acetate (Sigma-Aldrich; Cat# 306967) was initially diluted by 100-fold or 1000-fold (vol/vol) in mineral oil (Sigma-Aldrich; Cat# 69794) and then placed in a glass bottle (100 μ l in 900 μl mineral oil), delivered at 200 ml/min, and mixed with purified air (1000 ml/min). The mixed air stream was presented to flies through a 1-cm-wide opening Teflon tube placed ~1 cm from their antennas, and controlled by a Teflon solenoid valves and synchronized with the image acquisition system by Arduino boards. Imaging was made using a commercial Olympus BX61WI two-photon microscope with a 25x NA: 1.05 water-immersion objective and a mode-locked Ti:Sapphirelaser (Mai tai) tuned to 950 nm. The Glomeruli were identified according to the previous established antennal lobe map50.
Surgery for in vivo imaging in awake mouse
The surgery was performed in 2 steps. First, we removed the skin on top of the head and attached the metal recording chamber. The mouse was then given 3-5 days to recover, after which it was habituated to head-fixation. Approximately 1 week after the initial surgery, we opened the skull over the primary visual cortex (centered ~2.5 lateral, ~1.5 mm anterior from lambda), and did several 100 nl pressure injections of 1/5 diluted virus in the posterior half of the opening. The craniotomy was fitted by a cranial window made from 3 mm circular coverslip and a 2 × 2 mm square cut out of a #2 coverslip, as described previously49.
Two-photon imaging and analysis
Imaging was performed starting 14-16 hours after the surgery. The awake mouse was head-fixed on circular treadmill and imaged using a custom built 2-p system using an InSight DS+ laser (Spectra Physics) and the ScanImage 5.1 software62. Images were acquired from individual cells (or small groups of cells when possible) continuously at either 30 Hz (512 × 512 pixels) or 60 Hz (256 × 256 pixels). The mouse was shown a stimulus consisting of 50 seconds of darkness followed by 10 seconds of expanding white circles appearing at random positions on the screen. All data analysis was done in Matlab (Mathworks). After automatic image alignment, each recording was manually inspected to make sure alignment is artifact free. ROIs were then manually drawn over the cell bodies and raw fluorescent traces were extracted. Fluorescent traces were filtered by a 2 second moving average window to reduce fluctuations. The fluoresce traces were then divided into 10 second segments corresponding to either periods of darkness or periods of visual stimulation and dF/F0 was calculated for each of them. The maximum dF/F0 was compared for periods with or without stimulation using a Wilcoxon rank sum test.
Statistical analysis
Statistical results were reported as mean±s.e.m. Animals or cells were randomly assigned into control or experimental groups and investigators were blinded to experiment treatments. Given the negative correlation between the variation and square root of sample number, n, the group sample size was typically set to be ~10-25 to optimize the power of statistical tests and efficiency. Statistical significances of the means (p<0.05; two sides) were determined using Wilcoxon and Mann-Whitney Rank Sum non-parametric tests for paired and unpaired samples, respectively. Statistical significances of the linear relationships of two data groups were determined using linear regression t tests provided the normality and constant variance tests passed.
AUTHOR CONTRIBUTIONS
J.J. Z. and Y.L. conceived the project. M.J. did GACh screening and optimization as well as its validation in cultured neurons and IPN slices. Y.L., Y.S. and H.J. designed and performed the work on transgenic flies. M.J. and J.F. preformed experiments related to calcium imaging, GPCR internalization Tango assay and FRET measurements. L. M. and L.Z. did in vivo imaging of GACh sensors in mouse visual cortex. M.L. supervised the imaging experiments on MHb-IPN brain slices. All authors contributed to data analysis. M.J., J.J. Z. and Y.L. wrote the manuscript with input from other authors.
Competing interests statement
The authors declare that they have no competing financial interests.
Acknowledgments
We thank Y. Rao for generous sharing of two-photon microscopy. We are also grateful to LQ Luo, S. Owen, Y. Rao and L. Nevin for critical reading of the manuscript. We thank Z. Ye for the help in art designing. This work was supported by the National Basic Research Program of China (973 Program; grant 2015CB856402) and The General Program of National Natural Science Foundation of China (project 31671118 and project 31371442) and the Junior Thousand Talents Program of China to Y.L.