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h-channels contribute to divergent electrophysiological properties of supragranular pyramidal neurons in human versus mouse cerebral cortex

Brian E Kalmbach, Anatoly Buchin, Jeremy A Miller, Trygve E Bakken, Rebecca D Hodge, Peter Chong, Rebecca de Frates, Kael Dai, Ryder P. Gwinn, Charles Cobbs, Andrew L Ko, Jeffrey G Ojemann, Daniel L Silbergeld, Christof Koch, Costas A. Anastassiou, Ed Lein, Jonathan T Ting
doi: https://doi.org/10.1101/312298
Brian E Kalmbach
1Allen Institute for Brain Science, Seattle, WA
2Department of Physiology and Biophysics, University of Washington, Seattle, WA
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  • For correspondence: briank@alleninstitute.org jonathant@alleninstitute.org
Anatoly Buchin
1Allen Institute for Brain Science, Seattle, WA
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Jeremy A Miller
1Allen Institute for Brain Science, Seattle, WA
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Trygve E Bakken
1Allen Institute for Brain Science, Seattle, WA
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Rebecca D Hodge
1Allen Institute for Brain Science, Seattle, WA
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Peter Chong
1Allen Institute for Brain Science, Seattle, WA
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Rebecca de Frates
1Allen Institute for Brain Science, Seattle, WA
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Kael Dai
1Allen Institute for Brain Science, Seattle, WA
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Ryder P. Gwinn
3Epilepsy Surgery and Functional Neurosurgery, Swedish Neuroscience Institute, Seattle, WA
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Charles Cobbs
4The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute, Seattle, WA
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Andrew L Ko
5Regional Epilepsy Center at Harborview Medical Center, Seattle, WA
6Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA
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Jeffrey G Ojemann
5Regional Epilepsy Center at Harborview Medical Center, Seattle, WA
6Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA
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Daniel L Silbergeld
6Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA
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Christof Koch
1Allen Institute for Brain Science, Seattle, WA
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Costas A. Anastassiou
1Allen Institute for Brain Science, Seattle, WA
7Department of Neurology, University of British Columbia, Vancouver, BC
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Ed Lein
1Allen Institute for Brain Science, Seattle, WA
6Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA
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Jonathan T Ting
1Allen Institute for Brain Science, Seattle, WA
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  • For correspondence: briank@alleninstitute.org jonathant@alleninstitute.org
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Summary

Gene expression studies suggest that differential ion channel expression contributes to differences in rodent versus human neuronal physiology. We tested whether h-channels more prominently contribute to the physiological properties of human compared to mouse supragranular pyramidal neurons. Single cell/nucleus RNA sequencing revealed ubiquitous HCN1-subunit expression in excitatory neurons in human, but not mouse supragranular layers. Using patch-clamp recordings, we found stronger h-channel-related membrane properties in supragranular pyramidal neurons in human temporal cortex, compared to mouse supragranular pyramidal neurons in temporal association area. The magnitude of these differences depended upon cortical depth and was largest in pyramidal neurons in deep L3. Additionally, pharmacologically blocking h-channels produced a larger change in membrane properties in human compared to mouse neurons. Finally, using biophysical modeling, we provided evidence that h-channels promote the transfer of theta frequencies from dendrite-to-soma in human L3 pyramidal neurons. Thus, h-channels contribute to between-species differences in a fundamental neuronal property.

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Posted May 02, 2018.
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h-channels contribute to divergent electrophysiological properties of supragranular pyramidal neurons in human versus mouse cerebral cortex
Brian E Kalmbach, Anatoly Buchin, Jeremy A Miller, Trygve E Bakken, Rebecca D Hodge, Peter Chong, Rebecca de Frates, Kael Dai, Ryder P. Gwinn, Charles Cobbs, Andrew L Ko, Jeffrey G Ojemann, Daniel L Silbergeld, Christof Koch, Costas A. Anastassiou, Ed Lein, Jonathan T Ting
bioRxiv 312298; doi: https://doi.org/10.1101/312298
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h-channels contribute to divergent electrophysiological properties of supragranular pyramidal neurons in human versus mouse cerebral cortex
Brian E Kalmbach, Anatoly Buchin, Jeremy A Miller, Trygve E Bakken, Rebecca D Hodge, Peter Chong, Rebecca de Frates, Kael Dai, Ryder P. Gwinn, Charles Cobbs, Andrew L Ko, Jeffrey G Ojemann, Daniel L Silbergeld, Christof Koch, Costas A. Anastassiou, Ed Lein, Jonathan T Ting
bioRxiv 312298; doi: https://doi.org/10.1101/312298

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