Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Global connectivity and function of descending spinal input revealed by 3D microscopy and retrograde transduction

Zimei Wang, Brian Maunze, Yufang Wang, Pantelis Tsoulfas, Murray G. Blackmore
doi: https://doi.org/10.1101/314237
Zimei Wang
aDepartment of Biomedical Sciences, Marquette University, 53201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brian Maunze
aDepartment of Biomedical Sciences, Marquette University, 53201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yufang Wang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pantelis Tsoulfas
bDepartment of Neurological Surgery, Miami Project to Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL 33136
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: murray.blackmore@marquette.edu ptsoulfa@med.miami.edu
Murray G. Blackmore
aDepartment of Biomedical Sciences, Marquette University, 53201
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: murray.blackmore@marquette.edu ptsoulfa@med.miami.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

The brain communicates with the spinal cord through numerous axon tracts that arise from discrete nuclei, transmit distinct functions, and often collateralize to facilitate the coordination of descending commands. In efforts to restore supraspinal connectivity after injury or disease, this complexity presents a major challenge to interpreting functional outcomes, while the wide distribution of supraspinal nuclei complicates the delivery of therapeutics. Here we harness retrograde viral vectors to overcome these challenges. We demonstrate highly efficient retrograde transduction by AAV2-Retro in adult female mice, and employ tissue clearing to visualize descending tracts and create a mesoscopic projectome for the spinal cord. Moreover, chemogenetic silencing of supraspinal neurons with retrograde vectors results in complete and reversible forelimb paralysis, illustrating effective modulation of supraspinal function. Retrograde efficacy persists even after spinal injury, highlighting therapeutic potential. These data provide a global view of supraspinal connectivity and illustrate the potential of retrograde vectors to parse the functional contributions of supraspinal inputs.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted May 11, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Global connectivity and function of descending spinal input revealed by 3D microscopy and retrograde transduction
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Global connectivity and function of descending spinal input revealed by 3D microscopy and retrograde transduction
Zimei Wang, Brian Maunze, Yufang Wang, Pantelis Tsoulfas, Murray G. Blackmore
bioRxiv 314237; doi: https://doi.org/10.1101/314237
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Global connectivity and function of descending spinal input revealed by 3D microscopy and retrograde transduction
Zimei Wang, Brian Maunze, Yufang Wang, Pantelis Tsoulfas, Murray G. Blackmore
bioRxiv 314237; doi: https://doi.org/10.1101/314237

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4383)
  • Biochemistry (9599)
  • Bioengineering (7093)
  • Bioinformatics (24865)
  • Biophysics (12615)
  • Cancer Biology (9958)
  • Cell Biology (14354)
  • Clinical Trials (138)
  • Developmental Biology (7950)
  • Ecology (12107)
  • Epidemiology (2067)
  • Evolutionary Biology (15989)
  • Genetics (10925)
  • Genomics (14743)
  • Immunology (9869)
  • Microbiology (23676)
  • Molecular Biology (9485)
  • Neuroscience (50872)
  • Paleontology (369)
  • Pathology (1539)
  • Pharmacology and Toxicology (2683)
  • Physiology (4016)
  • Plant Biology (8657)
  • Scientific Communication and Education (1509)
  • Synthetic Biology (2397)
  • Systems Biology (6436)
  • Zoology (1346)