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CRISPR-Cas9 interference in cassava linked to the evolution of editing-resistant geminiviruses

View ORCID ProfileDevang Mehta, View ORCID ProfileAlessandra Stürchler, Matthias Hirsch-Hoffmann, Wilhelm Gruissem, View ORCID ProfileHervé Vanderschuren
doi: https://doi.org/10.1101/314542
Devang Mehta
1Plant Biotechnology, Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, Universitätstrasse 2, Zurich, 8092, Switzerland
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  • ORCID record for Devang Mehta
Alessandra Stürchler
1Plant Biotechnology, Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, Universitätstrasse 2, Zurich, 8092, Switzerland
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Matthias Hirsch-Hoffmann
1Plant Biotechnology, Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, Universitätstrasse 2, Zurich, 8092, Switzerland
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Wilhelm Gruissem
1Plant Biotechnology, Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, Universitätstrasse 2, Zurich, 8092, Switzerland
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Hervé Vanderschuren
1Plant Biotechnology, Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, Universitätstrasse 2, Zurich, 8092, Switzerland
2Plant Genetics, AgroBioChem Department, University of Liège, Passage des Déportés 2, Gembloux, 5030, Belgium (current address)
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ABSTRACT

We used CRISPR-Cas9 in the staple food crop cassava with the aim of engineering resistance to African cassava mosaic virus, a member of a widespread and important family of plant-pathogenic DNA viruses. We found that between 33 and 48% of edited virus genomes evolved a conserved single-nucleotide mutation that confers resistance to CRISPR-Cas9 cleavage. Our study highlights the potential for virus escape from this technology. Care should be taken to design CRISPR-Cas9 experiments that minimize the risk of virus escape.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted May 05, 2018.
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CRISPR-Cas9 interference in cassava linked to the evolution of editing-resistant geminiviruses
Devang Mehta, Alessandra Stürchler, Matthias Hirsch-Hoffmann, Wilhelm Gruissem, Hervé Vanderschuren
bioRxiv 314542; doi: https://doi.org/10.1101/314542
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CRISPR-Cas9 interference in cassava linked to the evolution of editing-resistant geminiviruses
Devang Mehta, Alessandra Stürchler, Matthias Hirsch-Hoffmann, Wilhelm Gruissem, Hervé Vanderschuren
bioRxiv 314542; doi: https://doi.org/10.1101/314542

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