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Direct programming of human mammary self-organised organoids by miR-106a-3p

View ORCID ProfileF. Delom, View ORCID ProfileM. Puceat, View ORCID ProfileD. Fessart
doi: https://doi.org/10.1101/314658
F. Delom
1Univ. Bordeaux, F-33000 Bordeaux, France
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  • For correspondence: delphine.fessart@yahoo.fr frederic.delom@yahoo.fr
M. Puceat
2Université Aix-Marseille, INSERM UMR_1251, F-13385 Marseille, France
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D. Fessart
1Univ. Bordeaux, F-33000 Bordeaux, France
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  • For correspondence: delphine.fessart@yahoo.fr frederic.delom@yahoo.fr
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Abstract

Organoids development relies on the self-organizing properties of adult stem cells to create structures which recapitulate the architecture, functionality, and genetic signature observed in original tissues. Little is known about of the exact nature of the intrinsic cell properties at the origin of organoid generation, and of the signaling pathways governing their differentiation. Herein, we carried out a functional microRNA screen to identify miRNAs at the origin of organoid generation from human epithelial cell culture. We uncover miR-106a-3p that initiates and promotes organoids. This miRNA acts as a master inducer of the expression of the three core pluripotency transcription factors (NANOG, OCT4 and SOX2) through the regulation of a set of 10 genes, and thus strengthening the reprogramming and cell differentiation of human epithelial cells into organoids. These data demonstrate that organoids can be directly generated from human epithelial cells by only one miRNA: miR-106a-3p. Hence, we appear to have identified a new determinant of organoid identity, which plays a role in reprogramming, cell differentiation and tissue engineering.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Lead contact: Delphine Fessart, delphine.fessart{at}yahoo.fr

  • Title has been changed for better accuracy

  • https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6594/

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 22, 2021.
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Direct programming of human mammary self-organised organoids by miR-106a-3p
F. Delom, M. Puceat, D. Fessart
bioRxiv 314658; doi: https://doi.org/10.1101/314658
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Direct programming of human mammary self-organised organoids by miR-106a-3p
F. Delom, M. Puceat, D. Fessart
bioRxiv 314658; doi: https://doi.org/10.1101/314658

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