Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

RsmV a small non-coding regulatory RNA in Pseudomonas aeruginosa that sequesters RsmA and RsmF from target mRNAs

Kayley H. Janssen, Manisha R. Diaz, Cindy J. Gode, Matthew C. Wolfgang, Timothy L. Yahr
doi: https://doi.org/10.1101/315341
Kayley H. Janssen
Department of Microbiology, University of Iowa
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Manisha R. Diaz
Department of Microbiology, University of Iowa
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cindy J. Gode
Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, NC
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matthew C. Wolfgang
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NCCystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, NC
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Timothy L. Yahr
Department of Microbiology, University of Iowa
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: tim-yahr@uiowa.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

The Gram-negative opportunistic pathogen Pseudomonas aeruginosa has distinct genetic programs that favor either acute or chronic virulence gene expression. Acute virulence is associated with twitching and swimming motility, expression of a type III secretion system (T3SS), and the absence of alginate, Psl, or Pel polysaccharide production. Traits associated with chronic infection include growth as a biofilm, reduced motility, and expression of a type VI secretion system (T6SS). The Rsm post-transcriptional regulatory system plays an important role in the inverse control of phenotypes associated with acute and chronic virulence. RsmA and RsmF are RNA-binding proteins that interact with target mRNAs to control gene expression at the post-transcriptional level. Previous work found that RsmA activity is controlled by at least three small, non-coding regulatory RNAs (RsmW, RsmY, and RsmZ). In this study, we took an in-silico approach to identify additional sRNAs that might function in the sequestration of RsmA and/or RsmF and identified RsmV, a 192 nt transcript with four predicted RsmA/RsmF consensus binding sites. RsmV is capable of sequestering RsmA and RsmF in vivo to activate translation of tssA1, a component of the T6SS, and to inhibit T3SS gene expression. Each of the predicted RsmA/RsmF consensus binding sites contribute to RsmV activity. Electrophoretic mobility shifts assays show that RsmF binds RsmV with >10-fold higher affinity than RsmY and RsmZ. Gene expression studies revealed that the temporal expression pattern of RsmV differs from RsmW, RsmY, and RsmZ. These findings suggest that each sRNA may play distinct roles in controlling RsmA and RsmF activity.

IMPORTANCE The role of RsmF in post-transcriptional control of gene expression remains enigmatic. While numerous rsmA-dependent phenotypes are more pronounced in an rsmAF double mutant, deletion of rsmF alone has only modest effects. Understanding mechanisms that control RsmF activity will provide insight into additional roles for RsmF. In the current study we identify RsmV as an sRNA that controls RsmA and RsmF activity, and show that RsmV, RsmW, RsmY, and RsmZ are differentially expressed during growth.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted May 07, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
RsmV a small non-coding regulatory RNA in Pseudomonas aeruginosa that sequesters RsmA and RsmF from target mRNAs
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
RsmV a small non-coding regulatory RNA in Pseudomonas aeruginosa that sequesters RsmA and RsmF from target mRNAs
Kayley H. Janssen, Manisha R. Diaz, Cindy J. Gode, Matthew C. Wolfgang, Timothy L. Yahr
bioRxiv 315341; doi: https://doi.org/10.1101/315341
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
RsmV a small non-coding regulatory RNA in Pseudomonas aeruginosa that sequesters RsmA and RsmF from target mRNAs
Kayley H. Janssen, Manisha R. Diaz, Cindy J. Gode, Matthew C. Wolfgang, Timothy L. Yahr
bioRxiv 315341; doi: https://doi.org/10.1101/315341

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (1527)
  • Biochemistry (2480)
  • Bioengineering (1739)
  • Bioinformatics (9683)
  • Biophysics (3903)
  • Cancer Biology (2972)
  • Cell Biology (4195)
  • Clinical Trials (135)
  • Developmental Biology (2627)
  • Ecology (4102)
  • Epidemiology (2031)
  • Evolutionary Biology (6898)
  • Genetics (5206)
  • Genomics (6501)
  • Immunology (2184)
  • Microbiology (6945)
  • Molecular Biology (2753)
  • Neuroscience (17282)
  • Paleontology (126)
  • Pathology (427)
  • Pharmacology and Toxicology (706)
  • Physiology (1057)
  • Plant Biology (2489)
  • Scientific Communication and Education (643)
  • Synthetic Biology (831)
  • Systems Biology (2690)
  • Zoology (430)