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A High-throughput Neurohistological Pipeline for Brain-Wide Mesoscale Connectivity Mapping of the Common Marmoset

Meng Kuan Lin, Yeonsook Shin Takahashi, Bing-Xing Huo, Mitsutoshi Hanada, Jaimi Nagashima, Junichi Hata, Alexander S. Tolpygo, Keerthi Ram, Brian Lee, Michael Miller, Marcello G.P. Rosa, Erika Sasaki, Atsushi Iriki, Hideyuki Okano, Partha P. Mitra
doi: https://doi.org/10.1101/315804
Meng Kuan Lin
1Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Japan.
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Yeonsook Shin Takahashi
1Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Japan.
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Bing-Xing Huo
1Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Japan.
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Mitsutoshi Hanada
1Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Japan.
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Jaimi Nagashima
1Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Japan.
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Junichi Hata
1Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Japan.
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Alexander S. Tolpygo
2Cold Spring Harbor Laboratory, Cold Spring Harbor, United States.
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Keerthi Ram
3Indian Institute of Technologies Madras, India.
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Brian Lee
4Center for Imaging Science, Johns Hopkins University, United States.
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Michael Miller
4Center for Imaging Science, Johns Hopkins University, United States.
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Marcello G.P. Rosa
5Department of Physiology and Biomedicine Discovery Institute, Monash University, Australia.
6Australian Research Council Centre of Excellence for Integrative Brain Function
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Erika Sasaki
7Central Institute for Experimental Animals, Japan.
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Atsushi Iriki
8Laboratory for Symbolic Cognitive Development, RIKEN Center for Brain Science, Japan.
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Hideyuki Okano
1Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Japan.
9Department of Physiology, Keio University School of Medicine, Japan.
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Partha P. Mitra
1Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Japan.
2Cold Spring Harbor Laboratory, Cold Spring Harbor, United States.
5Department of Physiology and Biomedicine Discovery Institute, Monash University, Australia.
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Abstract

Understanding the connectivity architecture of entire vertebrate brains is a fundamental but difficult task. MRI based methods offer whole brain coverage, but remain indirect in the approach to connectivity mapping. Recent progress has been made in directly mapping whole-brain connectivity architecture in the mouse at the mesoscopic scale. The basic approach uses tracer injections systematically placed on a grid of locations spanning the brain and computational analysis of the resulting whole brain data sets. Scaling this approach to bigger primate brains poses nontrivial technical challenges. Here we present an integrated neurohistological pipeline as well as a grid-based tracer injection strategy for systematic mesoscale connectivity mapping in the common Marmoset (Callithrix jacchus). Individual brains are sectioned into ~1700 20μm sections using the tape transfer technique, permitting high quality 3D reconstruction of a series of histochemical stains (Nissl, myelin) interleaved with tracer labelled sections. Combining the resulting 3D volumes, containing informative cytoarchitectonic markers, with in-vivo and ex-vivo MRI, and using an integrated computational pipeline, we are able to overcome the significant individual variation exhibited by Marmosets to obtain routine and high quality maps to a common atlas framework. This will facilitate the systematic assembly of a mesoscale connectivity matrix together with unprecedented 3D reconstructions of brain-wide projection patterns in a primate brain. While component instruments or protocols may be available from previous work, we believe that this is the first detailed systems-level presentation of the methodology required for high-throughput neuroanatomy in a model primate.

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Posted May 08, 2018.
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A High-throughput Neurohistological Pipeline for Brain-Wide Mesoscale Connectivity Mapping of the Common Marmoset
Meng Kuan Lin, Yeonsook Shin Takahashi, Bing-Xing Huo, Mitsutoshi Hanada, Jaimi Nagashima, Junichi Hata, Alexander S. Tolpygo, Keerthi Ram, Brian Lee, Michael Miller, Marcello G.P. Rosa, Erika Sasaki, Atsushi Iriki, Hideyuki Okano, Partha P. Mitra
bioRxiv 315804; doi: https://doi.org/10.1101/315804
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A High-throughput Neurohistological Pipeline for Brain-Wide Mesoscale Connectivity Mapping of the Common Marmoset
Meng Kuan Lin, Yeonsook Shin Takahashi, Bing-Xing Huo, Mitsutoshi Hanada, Jaimi Nagashima, Junichi Hata, Alexander S. Tolpygo, Keerthi Ram, Brian Lee, Michael Miller, Marcello G.P. Rosa, Erika Sasaki, Atsushi Iriki, Hideyuki Okano, Partha P. Mitra
bioRxiv 315804; doi: https://doi.org/10.1101/315804

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