Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The Effect of Berberine on the Transcriptome and Proteome of E. coli

Zhang Shilei, Jia Ze, Zhai Xianghe, Wang Chunguang, Zhang Tie
doi: https://doi.org/10.1101/318733
Zhang Shilei
Agricultural University of Hebei
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jia Ze
Agricultural University of Hebei
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhai Xianghe
Agricultural University of Hebei
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wang Chunguang
Agricultural University of Hebei
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhang Tie
Agricultural University of Hebei
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: zhangtie1998@163.com
  • Abstract
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Berberine is commonly used to treat diarrhea in China, and the antibacterial properties of berberine have been confirmed. High-throughput sequencing technology was used to explore the changes induced in E. coli by berberine. After treatment with berberine, the expression of RstA and YbjG were found to be significantly different by RNA-seq and quantitative proteomics. However, the levels of MdtA, PmrA, LolD, LptG, MlaB, RcsF, and DppB were found to be significantly different by quantitative proteomics. Transcriptome sequencing did not yield as many results as proteome sequencing. The results of small RNA prediction showed increased sRNA00002 levels. The study showed that the differentially expressed mRNAs and proteins were associated with multidrug-resistant efflux systems. It can be inferred that berberine reduces E. coli antibiotic resistance. The results of this study are undoubtedly valuable to other researchers.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted May 09, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The Effect of Berberine on the Transcriptome and Proteome of E. coli
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
The Effect of Berberine on the Transcriptome and Proteome of E. coli
Zhang Shilei, Jia Ze, Zhai Xianghe, Wang Chunguang, Zhang Tie
bioRxiv 318733; doi: https://doi.org/10.1101/318733
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
The Effect of Berberine on the Transcriptome and Proteome of E. coli
Zhang Shilei, Jia Ze, Zhai Xianghe, Wang Chunguang, Zhang Tie
bioRxiv 318733; doi: https://doi.org/10.1101/318733

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (999)
  • Biochemistry (1493)
  • Bioengineering (946)
  • Bioinformatics (6831)
  • Biophysics (2429)
  • Cancer Biology (1792)
  • Cell Biology (2530)
  • Clinical Trials (106)
  • Developmental Biology (1700)
  • Ecology (2576)
  • Epidemiology (1496)
  • Evolutionary Biology (5029)
  • Genetics (3623)
  • Genomics (4638)
  • Immunology (1175)
  • Microbiology (4252)
  • Molecular Biology (1629)
  • Neuroscience (10801)
  • Paleontology (83)
  • Pathology (240)
  • Pharmacology and Toxicology (409)
  • Physiology (555)
  • Plant Biology (1459)
  • Scientific Communication and Education (412)
  • Synthetic Biology (542)
  • Systems Biology (1878)
  • Zoology (260)