Abstract
Berberine is commonly used to treat diarrhea in China, and the antibacterial properties of berberine have been confirmed. High-throughput sequencing technology was used to explore the changes induced in E. coli by berberine. After treatment with berberine, the expression of RstA and YbjG were found to be significantly different by RNA-seq and quantitative proteomics. However, the levels of MdtA, PmrA, LolD, LptG, MlaB, RcsF, and DppB were found to be significantly different by quantitative proteomics. Transcriptome sequencing did not yield as many results as proteome sequencing. The results of small RNA prediction showed increased sRNA00002 levels. The study showed that the differentially expressed mRNAs and proteins were associated with multidrug-resistant efflux systems. It can be inferred that berberine reduces E. coli antibiotic resistance. The results of this study are undoubtedly valuable to other researchers.
Footnotes
This study was conducted at Hebei Agricultural University.
The procedures in this study were approved by the Institutional Animal Care and Ethics Committee of Agricultural University of Hebei (approval no. IACECHEBAU20150315).
This study was financially supported by the National Natural Science Foundation of China (No. 31572560); the Hebei Key Technology R&D Program grant (No. 16222701D) from the Department of Science & Technology, Hebei Province, China. We are grateful to Li Yan for editing this article.
