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Mechanosensitive clathrin platforms anchor desmin intermediate filaments in skeletal muscle

View ORCID ProfileAgathe Franck, Jeanne Lainé, View ORCID ProfileGilles Moulay, View ORCID ProfileMichaël Trichet, View ORCID ProfileChristel Gentil, Anaïs Fongy, View ORCID ProfileAnne Bigot, View ORCID ProfileSofia Benkhelifa-Ziyyat, Emmanuelle Lacène, Mai Thao Bui, View ORCID ProfileGuy Brochier, View ORCID ProfilePascale Guicheney, View ORCID ProfileSabrina Sacconi, View ORCID ProfileVincent Mouly, View ORCID ProfileNorma Romero, View ORCID ProfileCatherine Coirault, View ORCID ProfileMarc Bitoun, View ORCID ProfileStéphane Vassilopoulos
doi: https://doi.org/10.1101/321885
Agathe Franck
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
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Jeanne Lainé
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
2Department of Physiology, Sorbonne Université, UPMC Univ Paris 06, Pitié-Salpêtrière Hospital, Paris, France.
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Gilles Moulay
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
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Michaël Trichet
3Institut de Biologie Paris-Seine, Sorbonne Université, UPMC Univ Paris 06, CNRS, FR3631, Paris, France.
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Christel Gentil
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
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Anaïs Fongy
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
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Anne Bigot
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
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Sofia Benkhelifa-Ziyyat
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
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Emmanuelle Lacène
4Neuromuscular Morphology Unit, Institute of Myology, Pitié-Salpêtrière Hospital, Paris, France.
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Mai Thao Bui
4Neuromuscular Morphology Unit, Institute of Myology, Pitié-Salpêtrière Hospital, Paris, France.
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Guy Brochier
4Neuromuscular Morphology Unit, Institute of Myology, Pitié-Salpêtrière Hospital, Paris, France.
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Pascale Guicheney
5INSERM UMRS_1166, Sorbonne Université, UPMC Univ Paris 06, Institute of Cardiometabolism and Nutrition, Paris, France.
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Sabrina Sacconi
6Université Côte Azur, Referent Center for Neuromuscular diseases, Nice University Hospital, Nice. France.
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Vincent Mouly
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
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Norma Romero
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
4Neuromuscular Morphology Unit, Institute of Myology, Pitié-Salpêtrière Hospital, Paris, France.
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Catherine Coirault
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
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Marc Bitoun
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
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Stéphane Vassilopoulos
1Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, F-75013, Paris, France.
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  • For correspondence: s.vassilopoulos@institut-myologie.org
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Abstract

Large flat clathrin plaques are stable features of the plasma membrane associated with sites of strong adhesion suggesting that they could also play a role in force transduction. Here, we analyzed how clathrin plaques interact with the cytoskeleton and how they respond to mechanical cues in skeletal muscle myotubes. We show that branched actin networks surrounding clathrin plaques are directly regulated by dynamin 2, anchor intermediate filaments and sequester YAP at the plasma membrane. Dynamin 2, clathrin and desmin intermediate filaments are all required for basal YAP nucleocytoplasmic distribution and efficient nuclear translocation in response to mechanical stimuli. Dynamin 2 mutations that are responsible for centronuclear myopathy in humans disorganize the desmin network and deregulate YAP signaling both in vitro and in vivo. Thus, clathrin plaques and associated dynamin 2 are defined here as a new sensor conveying mechanical cues and integrate cell signaling with cytoskeletal regulation.

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Posted July 18, 2018.
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Mechanosensitive clathrin platforms anchor desmin intermediate filaments in skeletal muscle
Agathe Franck, Jeanne Lainé, Gilles Moulay, Michaël Trichet, Christel Gentil, Anaïs Fongy, Anne Bigot, Sofia Benkhelifa-Ziyyat, Emmanuelle Lacène, Mai Thao Bui, Guy Brochier, Pascale Guicheney, Sabrina Sacconi, Vincent Mouly, Norma Romero, Catherine Coirault, Marc Bitoun, Stéphane Vassilopoulos
bioRxiv 321885; doi: https://doi.org/10.1101/321885
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Mechanosensitive clathrin platforms anchor desmin intermediate filaments in skeletal muscle
Agathe Franck, Jeanne Lainé, Gilles Moulay, Michaël Trichet, Christel Gentil, Anaïs Fongy, Anne Bigot, Sofia Benkhelifa-Ziyyat, Emmanuelle Lacène, Mai Thao Bui, Guy Brochier, Pascale Guicheney, Sabrina Sacconi, Vincent Mouly, Norma Romero, Catherine Coirault, Marc Bitoun, Stéphane Vassilopoulos
bioRxiv 321885; doi: https://doi.org/10.1101/321885

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