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LAESI mass spectrometry imaging as a tool to differentiate the root metabolome of native and range-expanding plant species

View ORCID ProfilePurva Kulkarni, Rutger A. Wilschut, Koen J.F. Verhoeven, Wim H. van der Putten, Paolina Garbeva
doi: https://doi.org/10.1101/322867
Purva Kulkarni
Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands
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  • ORCID record for Purva Kulkarni
  • For correspondence: P.Kulkarni@nioo.knaw.nl
Rutger A. Wilschut
Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands
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Koen J.F. Verhoeven
Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands
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Wim H. van der Putten
Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands
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Paolina Garbeva
Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands
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ABSTRACT

Our understanding of chemical diversity in biological samples has greatly improved through recent advances in mass spectrometry (MS). MS-based-imaging (MSI) techniques have further enhanced this by providing spatial information on the distribution of metabolites and their relative abundance.

This study aims to employ laser-assisted electrospray ionization (LAESI) MSI as a tool to profile and compare the root metabolome of two pairs of native and range expanding plant species.

It has been proposed that successful range-expanding plant species, like introduced exotic invaders, have a novel, or a more diverse secondary chemistry. Although some tests have been made using aboveground plant materials, tests using root materials are rare. We tested the hypothesis that range-expanding plants possess more diverse root chemistries than native plant species.

To examine the root chemistry of the selected plant species, LAESI-MSI was performed in positive ion mode and data was acquired in a mass range of m/z 50-1200 with a spatial resolution of 100 µm. The acquired data was analyzed using in-house scripts, and differences in the spatial profiles were studied for discriminatory mass features. The results revealed clear differences in the metabolite profiles amongst and within both pairs of congeneric plant species, in the form of distinct metabolic fingerprints. The use of ambient conditions and the fact that no sample preparation was required, established LAESI-MSI as an ideal technique for untargeted metabolomics and for direct correlation of the acquired data to the underlying metabolomic complexity present in intact plant samples.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 15, 2018.
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LAESI mass spectrometry imaging as a tool to differentiate the root metabolome of native and range-expanding plant species
Purva Kulkarni, Rutger A. Wilschut, Koen J.F. Verhoeven, Wim H. van der Putten, Paolina Garbeva
bioRxiv 322867; doi: https://doi.org/10.1101/322867
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LAESI mass spectrometry imaging as a tool to differentiate the root metabolome of native and range-expanding plant species
Purva Kulkarni, Rutger A. Wilschut, Koen J.F. Verhoeven, Wim H. van der Putten, Paolina Garbeva
bioRxiv 322867; doi: https://doi.org/10.1101/322867

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