Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Nucleo-cytoplasmic trafficking regulates nuclear surface area during nuclear organogenesis

View ORCID ProfileVincent Boudreau, James Hazel, Jake Sellinger, Pan Chen, Kathryn Manakova, Rochelle Radzyminski, Hernan Garcia, Jun Allard, Jesse Gatlin, View ORCID ProfilePaul Maddox
doi: https://doi.org/10.1101/326140
Vincent Boudreau
1Department of Biology, University of North Carolina at Chapel Hill
2Physiology Course, Marine Biological Laboratory
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Vincent Boudreau
James Hazel
3Department of Molecular Biology, University of Wyoming
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jake Sellinger
1Department of Biology, University of North Carolina at Chapel Hill
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pan Chen
3Department of Molecular Biology, University of Wyoming
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kathryn Manakova
4Center for Complex Biological Systems, University of California, Irvine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rochelle Radzyminski
5Department of Physics, University of California, Irvine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hernan Garcia
2Physiology Course, Marine Biological Laboratory
7Department of Physics, University of California at Berkeley, Berkeley, California
8Department of Molecular & Cell Biology,University of California at Berkeley, Berkeley, California
9Institute for Quantitative Biosciences-QB3, University of California at Berkeley, Berkeley, California
10Biophysics Graduate Group, University of California at Berkeley, Berkeley, California
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jun Allard
4Center for Complex Biological Systems, University of California, Irvine
5Department of Physics, University of California, Irvine
6Department of Mathematics, University of California, Irvine
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jesse Gatlin
3Department of Molecular Biology, University of Wyoming
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paul Maddox
1Department of Biology, University of North Carolina at Chapel Hill
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Paul Maddox
  • For correspondence: pmaddox@unc.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Throughout development, nuclei must be assembled following every cell division to establish a functional organelle from compact, mitotic chromatin. During nuclear organogenesis, chromatin expands to establish a nucleus of a given size seperate from the cytoplasm. Determining how nuclear organogenesis is regulated is particularly significant in the context of certain cancers in which scaling relationships between cell and nuclear sizes are not maintained. Controlling cell size in vitro using a microfluidics approach, we determined that neither nuclear volume nor surface area scale directly with cell size. Looking to explain differential nuclear scaling relationships, we developed a simple mechano-chemical mathematical model. In simulating biological perturbations in silico, our model predicted crucial roles for nucleo-cytoplasmic trafficking in regulating nuclear expansion and in restricting the recruitment of a potential nuclear surface area factor. In mammalian tissue culture, inhibiting nuclear export increased nuclear expansion rates and reduced the amount of nuclear lamin, a candidate surface area factor, being recruited to assembling nuclei, supporting our model’s predictions. Targeting the principal nuclear export component in the Drosophila syncytial embryo, Embargoed, we show that nuclear expansion rates are also increased in this developmental context, consistent with our model. Using the MS2-reporter system in fly embryos, we demonstrate a role for nuclear export in regulating transcription activation timing and dynamics, suggesting that regulating nuclear assembly is crucial for downstream nuclear function. Taken together, we propose a simple model through which nuclear organogenesis is achieved and demonstrate a role for nuclear export in regulating nuclear assembly.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted May 18, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Nucleo-cytoplasmic trafficking regulates nuclear surface area during nuclear organogenesis
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Nucleo-cytoplasmic trafficking regulates nuclear surface area during nuclear organogenesis
Vincent Boudreau, James Hazel, Jake Sellinger, Pan Chen, Kathryn Manakova, Rochelle Radzyminski, Hernan Garcia, Jun Allard, Jesse Gatlin, Paul Maddox
bioRxiv 326140; doi: https://doi.org/10.1101/326140
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Nucleo-cytoplasmic trafficking regulates nuclear surface area during nuclear organogenesis
Vincent Boudreau, James Hazel, Jake Sellinger, Pan Chen, Kathryn Manakova, Rochelle Radzyminski, Hernan Garcia, Jun Allard, Jesse Gatlin, Paul Maddox
bioRxiv 326140; doi: https://doi.org/10.1101/326140

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2635)
  • Biochemistry (5224)
  • Bioengineering (3650)
  • Bioinformatics (15730)
  • Biophysics (7218)
  • Cancer Biology (5597)
  • Cell Biology (8051)
  • Clinical Trials (138)
  • Developmental Biology (4739)
  • Ecology (7472)
  • Epidemiology (2059)
  • Evolutionary Biology (10531)
  • Genetics (7704)
  • Genomics (10090)
  • Immunology (5157)
  • Microbiology (13829)
  • Molecular Biology (5354)
  • Neuroscience (30605)
  • Paleontology (212)
  • Pathology (872)
  • Pharmacology and Toxicology (1520)
  • Physiology (2235)
  • Plant Biology (4987)
  • Scientific Communication and Education (1037)
  • Synthetic Biology (1380)
  • Systems Biology (4131)
  • Zoology (804)