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Proteomic Analysis of NRROS Interactome Reveals the Presence of Chaperones and Mediators of the ERAD Pathway

View ORCID ProfileRajkumar Noubade, Qui Phung, Wilson Phung, Erik Verschueren, Laura Lau, Hiroyasu Konno, Jennie Lill, Wenjun Ouyang
doi: https://doi.org/10.1101/326512
Rajkumar Noubade
1Department of Immunology, Lipidomics Department, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
3Department of Inflammation and Oncology, Amgen Discovery Research., 1120 Veterans Blvd, South San Francisco, CA 94080, USA.
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  • ORCID record for Rajkumar Noubade
  • For correspondence: rnoubade@amgen.com qui.phung@gene.com
Qui Phung
2Microchemistry, Proteomics, Lipidomics Department, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
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  • For correspondence: rnoubade@amgen.com qui.phung@gene.com
Wilson Phung
2Microchemistry, Proteomics, Lipidomics Department, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
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Erik Verschueren
2Microchemistry, Proteomics, Lipidomics Department, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
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Laura Lau
3Department of Inflammation and Oncology, Amgen Discovery Research., 1120 Veterans Blvd, South San Francisco, CA 94080, USA.
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Hiroyasu Konno
3Department of Inflammation and Oncology, Amgen Discovery Research., 1120 Veterans Blvd, South San Francisco, CA 94080, USA.
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Jennie Lill
2Microchemistry, Proteomics, Lipidomics Department, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
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Wenjun Ouyang
1Department of Immunology, Lipidomics Department, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA.
3Department of Inflammation and Oncology, Amgen Discovery Research., 1120 Veterans Blvd, South San Francisco, CA 94080, USA.
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Abstract

Negative regulator of reactive oxygen species (NRROS, previously called LRRC33) is a leucine-rich repeat (LRR) domain containing, ER-resident transmembrane protein expressed primarily in lymphoid organs, especially in myeloid cells. We have previously demonstrated that NRROS regulates reactive oxygen species production by phagocytic cells by mediating degradation of NOX2 (gp91phox), a component of NOX2 complex responsible for the oxidative burst in these cells. Since LRR is the only functional domain in NRROS, it is likely to interact with other proteins for its biological functions. Here, by performing immunoprecipitation of NRROS and mass spectrometric analysis, we describe the NRROS interactome in macrophages and demonstrate that NRROS interacts with molecular chaperones/co-chaperones and mediators of the endoplasmic reticulum associated degradation (ERAD) pathway such as calnexin, suggesting a broader role for NRROS in protein biosynthesis and the ER quality control machinery.

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Posted May 20, 2018.
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Proteomic Analysis of NRROS Interactome Reveals the Presence of Chaperones and Mediators of the ERAD Pathway
Rajkumar Noubade, Qui Phung, Wilson Phung, Erik Verschueren, Laura Lau, Hiroyasu Konno, Jennie Lill, Wenjun Ouyang
bioRxiv 326512; doi: https://doi.org/10.1101/326512
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Proteomic Analysis of NRROS Interactome Reveals the Presence of Chaperones and Mediators of the ERAD Pathway
Rajkumar Noubade, Qui Phung, Wilson Phung, Erik Verschueren, Laura Lau, Hiroyasu Konno, Jennie Lill, Wenjun Ouyang
bioRxiv 326512; doi: https://doi.org/10.1101/326512

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