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Identification of a post-transcriptional regulatory element that responds to glucose in the African trypanosome

Yijian Qiu, Vijay Shankar, Rooksana E. Noorai, Nelson Yeung, Sarah Grace McAlpine, James Morris
doi: https://doi.org/10.1101/327346
Yijian Qiu
Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center, Clemson University, Clemson, SC, 29634, US
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Vijay Shankar
Clemson University Genomics & Computational Biology Laboratory, Clemson University, Clemson, SC, 29634, US
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Rooksana E. Noorai
Clemson University Genomics & Computational Biology Laboratory, Clemson University, Clemson, SC, 29634, US
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Nelson Yeung
Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center, Clemson University, Clemson, SC, 29634, US
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Sarah Grace McAlpine
Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center, Clemson University, Clemson, SC, 29634, US
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James Morris
Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center, Clemson University, Clemson, SC, 29634, US
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  • For correspondence: jmorri2@clemson.edu
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ABSTRACT

The ability to adapt to varying nutrient availability in changing environments is critical for successful parasitism. The lifecycle stages of the African trypanosome, Trypanosoma brucei, that infect the host mammalian bloodstream utilize glucose exclusively for ATP production. The finding that trypanosomes also inhabit other tissues that frequently contain lower glucose concentrations suggests blood stage parasites may have to respond to a dynamic environment with changing nutrient availability in order to survive. However, little is known about how the parasites coordinate gene expression with nutrient availability. Through transcriptome analysis, we have found blood stage parasites deprived of glucose alter gene expression in a pattern similar to transcriptome changes triggered by other stresses. A surprisingly low concentration of glucose (<10 μM) was required to initiate the response. To further understand the dynamic regulation of gene expression that occurs in response to altered glucose availability in the environment, we have interrogated the 3’UTR of cytochrome c oxidase subunit VI, a known lifecycle stage regulated gene, and have identified a stem-loop structure that confers glucose-responsive regulation at the translational level.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 24, 2018.
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Identification of a post-transcriptional regulatory element that responds to glucose in the African trypanosome
Yijian Qiu, Vijay Shankar, Rooksana E. Noorai, Nelson Yeung, Sarah Grace McAlpine, James Morris
bioRxiv 327346; doi: https://doi.org/10.1101/327346
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Identification of a post-transcriptional regulatory element that responds to glucose in the African trypanosome
Yijian Qiu, Vijay Shankar, Rooksana E. Noorai, Nelson Yeung, Sarah Grace McAlpine, James Morris
bioRxiv 327346; doi: https://doi.org/10.1101/327346

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