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Direct preparation of Cas9 ribonucleoprotein from E. coli for PCR-free seamless DNA assembly
Wenqiang Li, Shuntang Li, Jie Qiao, Fei Wang, Yang Liu, Ruyi He, Yi Liu, Lixin Ma
doi: https://doi.org/10.1101/328468
Wenqiang Li
1Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan, 430062 Hubei, China.
Shuntang Li
1Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan, 430062 Hubei, China.
Jie Qiao
1Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan, 430062 Hubei, China.
2Hubei Key Laboratory of Industrial Biotechnology, College of Life Science, Hubei University, Wuhan, 430062 Hubei, China.
Fei Wang
1Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan, 430062 Hubei, China.
2Hubei Key Laboratory of Industrial Biotechnology, College of Life Science, Hubei University, Wuhan, 430062 Hubei, China.
Yang Liu
2Hubei Key Laboratory of Industrial Biotechnology, College of Life Science, Hubei University, Wuhan, 430062 Hubei, China.
Ruyi He
1Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan, 430062 Hubei, China.
Yi Liu
1Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan, 430062 Hubei, China.
2Hubei Key Laboratory of Industrial Biotechnology, College of Life Science, Hubei University, Wuhan, 430062 Hubei, China.
Lixin Ma
1Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan, 430062 Hubei, China.
2Hubei Key Laboratory of Industrial Biotechnology, College of Life Science, Hubei University, Wuhan, 430062 Hubei, China.
Article usage
Posted June 07, 2018.
Direct preparation of Cas9 ribonucleoprotein from E. coli for PCR-free seamless DNA assembly
Wenqiang Li, Shuntang Li, Jie Qiao, Fei Wang, Yang Liu, Ruyi He, Yi Liu, Lixin Ma
bioRxiv 328468; doi: https://doi.org/10.1101/328468
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