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Luciferase of the Japanese syllid polychaete Odontosyllis umdecimdonta

View ORCID ProfileDarrin T. Schultz, Alexey A. Kotlobay, View ORCID ProfileRustam Ziganshin, Artyom Bannikov, Nadezhda M. Markina, Tatiana V. Chepurnyh, Ekaterina S. Shakhova, Ksenia Palkina, Steven H.D. Haddock, View ORCID ProfileIlia V. Yampolsky, Yuichi Oba
doi: https://doi.org/10.1101/329631
Darrin T. Schultz
1Department of Biomolecular Engineering and Bioinformatics, University of California Santa Cruz, 1156 High Street, Santa Cruz, California 95064, United States
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  • ORCID record for Darrin T. Schultz
Alexey A. Kotlobay
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya, 16/10, Moscow, Russian Federation 117997
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Rustam Ziganshin
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya, 16/10, Moscow, Russian Federation 117997
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Artyom Bannikov
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya, 16/10, Moscow, Russian Federation 117997
3Planta LLC, Bolshoi Boulevard, 42 Str 1, Office 335; Moscow, Russia 121205
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Nadezhda M. Markina
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya, 16/10, Moscow, Russian Federation 117997
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Tatiana V. Chepurnyh
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya, 16/10, Moscow, Russian Federation 117997
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Ekaterina S. Shakhova
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya, 16/10, Moscow, Russian Federation 117997
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Ksenia Palkina
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya, 16/10, Moscow, Russian Federation 117997
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Steven H.D. Haddock
4Monterey Bay Aquarium Research Institute, 7700 Sandholdt Road, Moss Landing, California 95039, United States
5Department of Ecology and Evolutionary Biology, University of California Santa Cruz, 1156 High Street, Santa Cruz, California 95064, United States
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Ilia V. Yampolsky
2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya, 16/10, Moscow, Russian Federation 117997
6Pirogov Russian National Research Medical University, Ostrovitianova 1, Moscow 117997, Russia
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  • For correspondence: ivyamp@ibch.ru yoba@isc.chubu.ac.jp
Yuichi Oba
7Department of Environmental Biology, Chubu University, Kasugai 487-8501, Japan
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  • For correspondence: ivyamp@ibch.ru yoba@isc.chubu.ac.jp
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1 Abstract

Odontosyllis undecimdonta is a marine syllid polychaete that produces bright internal and exuded bioluminescence. Despite over fifty years of biochemical investigation into Odontosyllis bioluminescence, the light-emitting small molecule substrate and catalyzing luciferase protein have remained a mystery. Here we describe the discovery of a bioluminescent protein fraction from O. undecimdonta, the identification of the luciferase using peptide and RNA sequencing, and the in vitro reconstruction of the bioluminescence reaction using highly purified O. undecimdonta luciferin and recombinant luciferase. Lastly, we found no identifiably homologous proteins in publicly available datasets. This suggests that the syllid polychaetes contain an evolutionarily unique luciferase among all characterized luminous taxa.

Highlights

  • The polychaete O. undecimdonta uses a luciferin-luciferase bioluminescence system

  • O. undecimdonta bioluminescence does not require additional cofactors

  • The luciferase of the Japanese fireworm is 329 amino acids long

  • Recombinant luciferase is not secreted when expressed in human cells

  • Exogenous luciferin does not seem to penetrate cell membranes-only lysate luminesces

  • The luciferase transcript is supported by full-length cDNA reads with 5’ and 3’ UTR

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 24, 2018.
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Luciferase of the Japanese syllid polychaete Odontosyllis umdecimdonta
Darrin T. Schultz, Alexey A. Kotlobay, Rustam Ziganshin, Artyom Bannikov, Nadezhda M. Markina, Tatiana V. Chepurnyh, Ekaterina S. Shakhova, Ksenia Palkina, Steven H.D. Haddock, Ilia V. Yampolsky, Yuichi Oba
bioRxiv 329631; doi: https://doi.org/10.1101/329631
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Luciferase of the Japanese syllid polychaete Odontosyllis umdecimdonta
Darrin T. Schultz, Alexey A. Kotlobay, Rustam Ziganshin, Artyom Bannikov, Nadezhda M. Markina, Tatiana V. Chepurnyh, Ekaterina S. Shakhova, Ksenia Palkina, Steven H.D. Haddock, Ilia V. Yampolsky, Yuichi Oba
bioRxiv 329631; doi: https://doi.org/10.1101/329631

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