Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Porcine Parvovirus VP1/VP2 on a Time Series Epitope Mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens

Ancelmo Rabelo de Souza, Marriam Yamin, Danielle Gava, Janice Reis Ciacci Zanella, Maria Sílvia Viccari Gatti, Carlos Francisco Sampaio Bonafe, Daniel Ferreira de Lima Neto
doi: https://doi.org/10.1101/330589
Ancelmo Rabelo de Souza
aDepartamento de Bioquímica e Biologia Tecidual e, Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 255, Cidade Universitária Zeferino Vaz, 13083-862, Campinas, SP, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marriam Yamin
aDepartamento de Bioquímica e Biologia Tecidual e, Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 255, Cidade Universitária Zeferino Vaz, 13083-862, Campinas, SP, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Danielle Gava
cEmbrapa Suínos e Aves, Laboratório de Virologia de Suínos, 89715-899, Concórdia, SC, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Janice Reis Ciacci Zanella
cEmbrapa Suínos e Aves, Laboratório de Virologia de Suínos, 89715-899, Concórdia, SC, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maria Sílvia Viccari Gatti
aDepartamento de Bioquímica e Biologia Tecidual e, Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 255, Cidade Universitária Zeferino Vaz, 13083-862, Campinas, SP, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carlos Francisco Sampaio Bonafe
aDepartamento de Bioquímica e Biologia Tecidual e, Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 255, Cidade Universitária Zeferino Vaz, 13083-862, Campinas, SP, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel Ferreira de Lima Neto
aDepartamento de Bioquímica e Biologia Tecidual e, Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 255, Cidade Universitária Zeferino Vaz, 13083-862, Campinas, SP, Brazil
bDepartamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 255, Cidade Universitária Zeferino Vaz, 13083-862, Campinas, SP, Brazil
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: danielviro@gmail.com
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Porcine parvovirus (PPV) is a DNA virus that causes reproductive failure in gilts and sows, resulting in embryonic and fetal losses worldwide. Epitope mapping of PPV is important for developing new vaccines. In this study, we used spot synthesis analysis for epitope mapping of the capsid proteins of PPV (NADL-2 strain) and correlated the findings with predictive data from immunoinformatics. The virus was exposed to three conditions prior to inoculation in pigs: native (untreated), high hydrostatic pressure (350 MPa for 1 h) at room temperature and high hydrostatic pressure (350 MPa for 1h) at −18 °C, compared with a commercial vaccine produced using inactivated PPV. The screening of serum samples detected 44 positive spots corresponding to 20 antigenic sites. Each type of inoculated antigen elicited a distinct epitope set. In silico prediction located linear and discontinuous epitopes in B cells that coincided with several epitopes detected in spot synthesis of sera from pigs that received different preparations of inoculum. The approach used here provided important information on the antibody/antigen interactions required to improve B cell responses to PPV and may be useful in developing novel strategies for producing new vaccines.

Abbreviations 3D, three dimensional; HHP, high hydrostatic pressure; ID, identification; IEDB, Immune Epitope Database; ORFs, open reading frames; p.i., post-infection; PPV, porcine parvovirus; RMSD, root-mean-square deviation of atomic positions; R(g), radius of gyration. RMSF, root-mean-square fluctuation; SK6, Swine kidney cell; SPF, specific pathogen free; TCID50/mL, Median Tissue Culture Infectious Dose; HI, Hemagglutination Inhibition.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted May 25, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Porcine Parvovirus VP1/VP2 on a Time Series Epitope Mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Porcine Parvovirus VP1/VP2 on a Time Series Epitope Mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens
Ancelmo Rabelo de Souza, Marriam Yamin, Danielle Gava, Janice Reis Ciacci Zanella, Maria Sílvia Viccari Gatti, Carlos Francisco Sampaio Bonafe, Daniel Ferreira de Lima Neto
bioRxiv 330589; doi: https://doi.org/10.1101/330589
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Porcine Parvovirus VP1/VP2 on a Time Series Epitope Mapping: exploring the effects of high hydrostatic pressure on the immune recognition of antigens
Ancelmo Rabelo de Souza, Marriam Yamin, Danielle Gava, Janice Reis Ciacci Zanella, Maria Sílvia Viccari Gatti, Carlos Francisco Sampaio Bonafe, Daniel Ferreira de Lima Neto
bioRxiv 330589; doi: https://doi.org/10.1101/330589

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3585)
  • Biochemistry (7539)
  • Bioengineering (5494)
  • Bioinformatics (20724)
  • Biophysics (10292)
  • Cancer Biology (7946)
  • Cell Biology (11609)
  • Clinical Trials (138)
  • Developmental Biology (6584)
  • Ecology (10161)
  • Epidemiology (2065)
  • Evolutionary Biology (13573)
  • Genetics (9511)
  • Genomics (12811)
  • Immunology (7900)
  • Microbiology (19490)
  • Molecular Biology (7632)
  • Neuroscience (41969)
  • Paleontology (307)
  • Pathology (1254)
  • Pharmacology and Toxicology (2189)
  • Physiology (3258)
  • Plant Biology (7017)
  • Scientific Communication and Education (1293)
  • Synthetic Biology (1946)
  • Systems Biology (5417)
  • Zoology (1112)