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Clarification and confocal imaging of the non-human primate placental micro-anatomy

View ORCID ProfileJames A. Sargent, Victoria HJ Roberts, Jessica Gaffney, Antonio E. Frias
doi: https://doi.org/10.1101/330647
James A. Sargent
1Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR.
2Oregon National Primate Research Center, Beaverton, OR.
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Victoria HJ Roberts
2Oregon National Primate Research Center, Beaverton, OR.
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Jessica Gaffney
2Oregon National Primate Research Center, Beaverton, OR.
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Antonio E. Frias
1Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR.
2Oregon National Primate Research Center, Beaverton, OR.
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Abstract

Placental function is essential for the development of the fetus, and is – in part – related to the 3D arrangement of the villous and vascular geometry. Recent advances in tissue clarification techniques allow for deep high-resolution imaging with confocal microscopy without altering the spatial characteristics of the tissue. These image stacks can be analyzed quantitatively to provide insights regarding the villous and vascular micro-anatomy as well as the interrelationships between the two. However, such analyses require optimization of the tissue preparation, immuno-labeling, and clarification protocol in order to provide reliable results suitable for the detection of subtle differences in pathologic pregnancies. Placental and fetal development are similar between human and non-human primate pregnancies, with the latter serving as a reliable, validated, highly-controlled, well-characterized translational model for the former.

We present a protocol for the preparation, immuno-labeling, and clarification of the non-human primate placenta optimized for confocal microscopy and subsequent quantification of the micro-anatomic structures.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 24, 2018.
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Clarification and confocal imaging of the non-human primate placental micro-anatomy
James A. Sargent, Victoria HJ Roberts, Jessica Gaffney, Antonio E. Frias
bioRxiv 330647; doi: https://doi.org/10.1101/330647
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Clarification and confocal imaging of the non-human primate placental micro-anatomy
James A. Sargent, Victoria HJ Roberts, Jessica Gaffney, Antonio E. Frias
bioRxiv 330647; doi: https://doi.org/10.1101/330647

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