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Commensal bacteria act as a broad genetic buffer in Drosophila during chronic under-nutrition

Dali Ma, Maroun Bou-Sleiman, Pauline Joncour, Claire-Emmanuelle Indelicato, Michael Frochaux, Virginie Braman, Maria Litovchenko, Gilles Storelli, View ORCID ProfileBart Deplancke, François Leulier
doi: https://doi.org/10.1101/334342
Dali Ma
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon 1, Unité Mixte de Recherche 5242, 69364 Lyon, Cedex 07, France
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Maroun Bou-Sleiman
2Laboratory of Systems Biology and Genetics, Institute of Bioengineering and Swiss Institute of Bioinformatics, School of Life Sciences Ecole Polytechnique Federale de Lausanne (EPFL) CH-1015, Lausanne, Switzerland
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Pauline Joncour
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon 1, Unité Mixte de Recherche 5242, 69364 Lyon, Cedex 07, France
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Claire-Emmanuelle Indelicato
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon 1, Unité Mixte de Recherche 5242, 69364 Lyon, Cedex 07, France
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Michael Frochaux
2Laboratory of Systems Biology and Genetics, Institute of Bioengineering and Swiss Institute of Bioinformatics, School of Life Sciences Ecole Polytechnique Federale de Lausanne (EPFL) CH-1015, Lausanne, Switzerland
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Virginie Braman
2Laboratory of Systems Biology and Genetics, Institute of Bioengineering and Swiss Institute of Bioinformatics, School of Life Sciences Ecole Polytechnique Federale de Lausanne (EPFL) CH-1015, Lausanne, Switzerland
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Maria Litovchenko
2Laboratory of Systems Biology and Genetics, Institute of Bioengineering and Swiss Institute of Bioinformatics, School of Life Sciences Ecole Polytechnique Federale de Lausanne (EPFL) CH-1015, Lausanne, Switzerland
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Gilles Storelli
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon 1, Unité Mixte de Recherche 5242, 69364 Lyon, Cedex 07, France
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Bart Deplancke
2Laboratory of Systems Biology and Genetics, Institute of Bioengineering and Swiss Institute of Bioinformatics, School of Life Sciences Ecole Polytechnique Federale de Lausanne (EPFL) CH-1015, Lausanne, Switzerland
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  • ORCID record for Bart Deplancke
  • For correspondence: bart.deplancke@epfl.ch francois.leulier@ens-lyon.fr
François Leulier
1Institut de Génomique Fonctionnelle de Lyon (IGFL), Université de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon 1, Unité Mixte de Recherche 5242, 69364 Lyon, Cedex 07, France
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  • For correspondence: bart.deplancke@epfl.ch francois.leulier@ens-lyon.fr
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Summary

Eukaryotic genomes encode several well-studied buffering mechanisms that robustly maintain invariant phenotypic outcome despite fluctuating environmental conditions. Here we show that the gut microbiota, represented by a single Drosophila facultative symbiont, Lactobacillus plantarum (LpWJL), acts also as a broad genetic buffer that masks the contribution of the cryptic genetic variations in the host under nutritional stress. During chronic under-nutrition, LpWJL consistently reduces variation in different host phenotypic traits and ensures robust organ patterning; LpWJL also decreases genotype-dependent expression variation, particularly for development-associated genes. We further demonstrate that LpWJL buffers via reactive oxygen species (ROS) signaling whose inhibition severely impairs microbiota-mediated phenotypic robustness. We thus identified an unexpected contribution of facultative symbionts to Drosophila fitness by assuring developmental robustness and phenotypic homogeneity in times of nutritional stress.

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Posted May 30, 2018.
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Commensal bacteria act as a broad genetic buffer in Drosophila during chronic under-nutrition
Dali Ma, Maroun Bou-Sleiman, Pauline Joncour, Claire-Emmanuelle Indelicato, Michael Frochaux, Virginie Braman, Maria Litovchenko, Gilles Storelli, Bart Deplancke, François Leulier
bioRxiv 334342; doi: https://doi.org/10.1101/334342
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Commensal bacteria act as a broad genetic buffer in Drosophila during chronic under-nutrition
Dali Ma, Maroun Bou-Sleiman, Pauline Joncour, Claire-Emmanuelle Indelicato, Michael Frochaux, Virginie Braman, Maria Litovchenko, Gilles Storelli, Bart Deplancke, François Leulier
bioRxiv 334342; doi: https://doi.org/10.1101/334342

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