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Measuring the archaeome: detection and quantification of archaea signatures in the human body

Manuela R. Pausan, Cintia Csorba, Georg Singer, Holger Till, Veronika Schöpf, Elisabeth Santigli, Barbara Klug, Christoph Högenauer, Marcus Blohs, Christine Moissl-Eichinger
doi: https://doi.org/10.1101/334748
Manuela R. Pausan
1Department of Internal Medicine, Medical University of Graz, Graz, Austria
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Cintia Csorba
1Department of Internal Medicine, Medical University of Graz, Graz, Austria
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Georg Singer
2Department of Pediatrics and Adolescent Surgery, Medical University of Graz, Graz, Austria
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Holger Till
2Department of Pediatrics and Adolescent Surgery, Medical University of Graz, Graz, Austria
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Veronika Schöpf
3Institute of Psychology, University of Graz, Graz, Austria
5BioTechMed, Graz, Austria
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Elisabeth Santigli
4Department of Dentistry and Maxillofacial Surgery, Medical University Graz, Graz, Austria
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Barbara Klug
4Department of Dentistry and Maxillofacial Surgery, Medical University Graz, Graz, Austria
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Christoph Högenauer
1Department of Internal Medicine, Medical University of Graz, Graz, Austria
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Marcus Blohs
1Department of Internal Medicine, Medical University of Graz, Graz, Austria
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Christine Moissl-Eichinger
1Department of Internal Medicine, Medical University of Graz, Graz, Austria
5BioTechMed, Graz, Austria
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  • For correspondence: christine.moissl-eichinger@medunigraz.at
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Abstract

Due to their fundamentally different biology, archaea are consistently overlooked in conventional 16S rRNA gene amplicon surveys. Herein, we evaluate different methodological set-ups to detect and quantify archaea signatures in human samples (nose, oral, appendix, stool, and skin) using amplicon sequencing and quantitative PCR.

With our optimized protocol, we were able to increase the detection of archaeal RSVs from one (using a so-called “universal” approach) to 81 RSVs in a representative sample set. Moreover, we confirmed the presence of about 5% archaeal signatures in the human gut, but found, unexpectedly, an almost 1:1 ratio of archaeal to bacterial 16S rRNA genes in appendix and nose samples. This finding indicates a high prevalence of archaeal signatures in body regions thus far not analyzed for the presence of archaea using appropriate methods.

In order to assess the archaeome diversity and archaeal abundance, a specific archaea-targeting methodology is required, for which we propose two standard procedures. These methodologies might not only prove useful for analyzing the human archaeome in more detail, but could also be used for other holobionts’ samples.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 30, 2018.
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Measuring the archaeome: detection and quantification of archaea signatures in the human body
Manuela R. Pausan, Cintia Csorba, Georg Singer, Holger Till, Veronika Schöpf, Elisabeth Santigli, Barbara Klug, Christoph Högenauer, Marcus Blohs, Christine Moissl-Eichinger
bioRxiv 334748; doi: https://doi.org/10.1101/334748
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Measuring the archaeome: detection and quantification of archaea signatures in the human body
Manuela R. Pausan, Cintia Csorba, Georg Singer, Holger Till, Veronika Schöpf, Elisabeth Santigli, Barbara Klug, Christoph Högenauer, Marcus Blohs, Christine Moissl-Eichinger
bioRxiv 334748; doi: https://doi.org/10.1101/334748

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