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Anti-diuretic activity of a CAPA neuropeptide can compromise Drosophila chill tolerance

View ORCID ProfileHeath A. MacMillan, Basma Nazal, Sahr Wali, Gil Y. Yerushalmi, Lidiya Misyura, Andrew Donini, Jean-Paul Paluzzi
doi: https://doi.org/10.1101/336057
Heath A. MacMillan
Department of Biology, York University, Toronto, Canada, M3J 1P3
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Basma Nazal
Department of Biology, York University, Toronto, Canada, M3J 1P3
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Sahr Wali
Department of Biology, York University, Toronto, Canada, M3J 1P3
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Gil Y. Yerushalmi
Department of Biology, York University, Toronto, Canada, M3J 1P3
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Lidiya Misyura
Department of Biology, York University, Toronto, Canada, M3J 1P3
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Andrew Donini
Department of Biology, York University, Toronto, Canada, M3J 1P3
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Jean-Paul Paluzzi
Department of Biology, York University, Toronto, Canada, M3J 1P3
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Abstract

For insects, chilling injuries that occur in the absence of freezing are often related to a systemic loss of ion and water balance that leads to extracellular hyperkalemia, cell depolarization, and the triggering of apoptotic signalling cascades. The ability of insect ionoregulatory organs (e.g. the Malpighian tubules) to maintain ion balance in the cold has been linked to improved chill tolerance, and many neuroendocrine factors are known to influence ion transport rates of these organs. Injection of micromolar doses of CAPA (an insect neuropeptide) have been previously demonstrated to improve Drosophila cold tolerance, but the mechanisms through which it impacts chill tolerance are unclear, and low doses of CAPA have been demonstrated to cause anti-diuresis in other insects, including dipterans. Here, we provide evidence that low (fM) and high (µM) doses of CAPA impair and improve chill tolerance, respectively, via two different effects on Malpighian tubule ion and water transport. While low doses of CAPA are anti-diuretic, reduce tubule K+ clearance rates and reduce chill tolerance, high doses facilitate K+ clearance from the haemolymph and increase chill tolerance. By quantifying CAPA peptide levels in the central nervous system, we estimated the maximum achievable hormonal titres of CAPA, and found evidence to suggest that CAPA may function as an anti-diuretic peptide in Drosophila. We provide the first evidence of a neuropeptide that can negatively affect cold tolerance in an insect, and the first evidence of CAPA as an anti-diuretic peptide in this ubiquitous insect model.

Summary Statement Many insects ion balance in the cold. We show how one neuropeptide can slow ion transport and reduce the cold tolerance of a fly.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted May 31, 2018.
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Anti-diuretic activity of a CAPA neuropeptide can compromise Drosophila chill tolerance
Heath A. MacMillan, Basma Nazal, Sahr Wali, Gil Y. Yerushalmi, Lidiya Misyura, Andrew Donini, Jean-Paul Paluzzi
bioRxiv 336057; doi: https://doi.org/10.1101/336057
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Anti-diuretic activity of a CAPA neuropeptide can compromise Drosophila chill tolerance
Heath A. MacMillan, Basma Nazal, Sahr Wali, Gil Y. Yerushalmi, Lidiya Misyura, Andrew Donini, Jean-Paul Paluzzi
bioRxiv 336057; doi: https://doi.org/10.1101/336057

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