Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Single-cell transcriptomics identifies CD44 as a new marker and regulator of haematopoietic stem cells development

Morgan Oatley, View ORCID ProfileÖzge Vargel Bölükbasi, Valentine Svensson, Maya Shvartsman, Kerstin Ganter, Katharina Zirngibl, Polina V. Pavlovich, Vladislava Milchevskaya, Vladimira Foteva, Kedar N. Natarajan, Bianka Baying, Vladimir Benes, View ORCID ProfileKiran R. Patil, View ORCID ProfileSarah A. Teichmann, View ORCID ProfileChristophe Lancrin
doi: https://doi.org/10.1101/338178
Morgan Oatley
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, Monterotondo, Italy.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Özge Vargel Bölükbasi
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, Monterotondo, Italy.
2Current address: Boston’s Children Hospital/Harvard Medical School, Boston, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Özge Vargel Bölükbasi
Valentine Svensson
3Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, UK.
4European Molecular Biology Laboratory, EMBL-EBI, Wellcome Genome Campus, Hinxton, UK.
5Current address: Pachter Lab, Caltech, California, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maya Shvartsman
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, Monterotondo, Italy.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kerstin Ganter
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, Monterotondo, Italy.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Katharina Zirngibl
6European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Polina V. Pavlovich
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, Monterotondo, Italy.
7Moscow Institute of Physics and Technology, Institutskii Per. 9, Moscow Region, Dolgoprudny 141700, Russia.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vladislava Milchevskaya
6European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany
8Current address: Institut für Medizinische Statistik und Bioinformatik, Köln, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vladimira Foteva
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, Monterotondo, Italy.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kedar N. Natarajan
3Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, UK.
9Current address: The University of Southern Denmark, Danish Institute for Advanced Study, Department of Biochemistry and Molecular Biology, Odense, Denmark.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bianka Baying
10European Molecular Biology Laboratory, Genomics Core Facility, Heidelberg, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vladimir Benes
10European Molecular Biology Laboratory, Genomics Core Facility, Heidelberg, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kiran R. Patil
6European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kiran R. Patil
Sarah A. Teichmann
3Wellcome Trust Sanger Institute, Wellcome Genome Campus, Hinxton, UK.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sarah A. Teichmann
Christophe Lancrin
1European Molecular Biology Laboratory, EMBL Rome - Epigenetics and Neurobiology Unit, Monterotondo, Italy.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Christophe Lancrin
  • For correspondence: christophe.lancrin@embl.it
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

The endothelial to haematopoietic transition (EHT) is the process whereby haemogenic endothelium differentiates into haematopoietic stem and progenitor cells (HSPCs). The intermediary steps of this process are unclear, in particular the identity of endothelial cells that give rise to HSPCs is unknown. Using single-cell transcriptome analysis and antibody screening we identified CD44 as a new marker of EHT enabling us to isolate robustly the different stages of EHT in the aorta gonad mesonephros (AGM) region. This allowed us to provide a very detailed phenotypical and transcriptional profile for haemogenic endothelial cells, characterising them with high expression of genes related to Notch signalling, TGFbeta/BMP antagonists (Smad6, Smad7 and Bmper) and a downregulation of genes related to glycolysis and the TCA cycle. Moreover, we demonstrated that by inhibiting the interaction between CD44 and its ligand hyaluronan we could block EHT, identifying a new regulator of HSPC development.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted June 06, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Single-cell transcriptomics identifies CD44 as a new marker and regulator of haematopoietic stem cells development
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Single-cell transcriptomics identifies CD44 as a new marker and regulator of haematopoietic stem cells development
Morgan Oatley, Özge Vargel Bölükbasi, Valentine Svensson, Maya Shvartsman, Kerstin Ganter, Katharina Zirngibl, Polina V. Pavlovich, Vladislava Milchevskaya, Vladimira Foteva, Kedar N. Natarajan, Bianka Baying, Vladimir Benes, Kiran R. Patil, Sarah A. Teichmann, Christophe Lancrin
bioRxiv 338178; doi: https://doi.org/10.1101/338178
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Single-cell transcriptomics identifies CD44 as a new marker and regulator of haematopoietic stem cells development
Morgan Oatley, Özge Vargel Bölükbasi, Valentine Svensson, Maya Shvartsman, Kerstin Ganter, Katharina Zirngibl, Polina V. Pavlovich, Vladislava Milchevskaya, Vladimira Foteva, Kedar N. Natarajan, Bianka Baying, Vladimir Benes, Kiran R. Patil, Sarah A. Teichmann, Christophe Lancrin
bioRxiv 338178; doi: https://doi.org/10.1101/338178

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4224)
  • Biochemistry (9101)
  • Bioengineering (6749)
  • Bioinformatics (23935)
  • Biophysics (12086)
  • Cancer Biology (9491)
  • Cell Biology (13728)
  • Clinical Trials (138)
  • Developmental Biology (7614)
  • Ecology (11656)
  • Epidemiology (2066)
  • Evolutionary Biology (15476)
  • Genetics (10615)
  • Genomics (14292)
  • Immunology (9456)
  • Microbiology (22773)
  • Molecular Biology (9069)
  • Neuroscience (48840)
  • Paleontology (354)
  • Pathology (1479)
  • Pharmacology and Toxicology (2562)
  • Physiology (3822)
  • Plant Biology (8307)
  • Scientific Communication and Education (1467)
  • Synthetic Biology (2289)
  • Systems Biology (6169)
  • Zoology (1297)