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Trio deep-sequencing does not reveal unexpected mutations in Cas9-edited monkeys

Xin Luo, Yaoxi He, Chao Zhang, Xiechao He, Lanzhen Yan, Min Li, Ting Hu, Yan Hu, Jin Jiang, Xiaoyu Meng, Weizhi Ji, Xudong Zhao, Ping Zheng, Shuhua Xu, Bing Su
doi: https://doi.org/10.1101/339143
Xin Luo
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Yaoxi He
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Chao Zhang
CAS-MPG Partner Institute for Computational Biology;
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Xiechao He
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Lanzhen Yan
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Min Li
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Ting Hu
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Yan Hu
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Jin Jiang
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Xiaoyu Meng
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Weizhi Ji
Institute of Primate Translational Medicine, Kunming University of Science and Technology;
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Xudong Zhao
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Ping Zheng
Kunming Institute of Zoology, Chinese Academy of Sciences;
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Shuhua Xu
CAS-MPG Partner Institute for Computational Biology;
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Bing Su
Kunming Institute of Zoology, CAS
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  • For correspondence: sub@mail.kiz.ac.cn
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Abstract

CRISPR-Cas9 is a widely-used genome editing tool, but its off-target effect remains a concern, especially in view of future clinical applications. Non-human primates (NHPs) share close genetic and physiological similarities with humans, making them an ideal preclinical model for developing Cas9-based therapies. However, no comprehensive in vivo off-target assessment has been conducted in NHPs. Here we performed whole genome trio sequencing of Cas9-treated monkeys. We found they only carried a small number of de novo mutations that can be explained by expected spontaneous mutations, and no unexpected mutations were detected.

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Posted June 05, 2018.
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Trio deep-sequencing does not reveal unexpected mutations in Cas9-edited monkeys
Xin Luo, Yaoxi He, Chao Zhang, Xiechao He, Lanzhen Yan, Min Li, Ting Hu, Yan Hu, Jin Jiang, Xiaoyu Meng, Weizhi Ji, Xudong Zhao, Ping Zheng, Shuhua Xu, Bing Su
bioRxiv 339143; doi: https://doi.org/10.1101/339143
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Trio deep-sequencing does not reveal unexpected mutations in Cas9-edited monkeys
Xin Luo, Yaoxi He, Chao Zhang, Xiechao He, Lanzhen Yan, Min Li, Ting Hu, Yan Hu, Jin Jiang, Xiaoyu Meng, Weizhi Ji, Xudong Zhao, Ping Zheng, Shuhua Xu, Bing Su
bioRxiv 339143; doi: https://doi.org/10.1101/339143

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