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Separation and Loss of Centrioles from Primordidal Germ Cells to Mature Oocytes in the Mouse

Gerald Schatten, Calvin R Simerly, Marion Manil-Ségalen, Carlos Castro, Carrie Hartnett, Dong Kong, View ORCID ProfileMarie-Helene Verlhac, Jadranka Loncarek
doi: https://doi.org/10.1101/339572
Gerald Schatten
Pittsburgh Development Center;
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  • For correspondence: gschatten@pdc.magee.edu
Calvin R Simerly
Pittsburgh Development Center;
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Marion Manil-Ségalen
College de France;
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Carlos Castro
University of Pittsburgh Medical School;
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Carrie Hartnett
Pittsburgh Development Center;
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Dong Kong
National Cancer Institute
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Marie-Helene Verlhac
College de France;
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  • ORCID record for Marie-Helene Verlhac
Jadranka Loncarek
National Cancer Institute
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Abstract

Mammalian oocytes lack centrioles, but how or at what stage mature eggs lose their centrioles during oogenesis is unknown. To address centriole disappearance during oogenesis, using a transgenic mouse expressing GFP-centrin-2 (GFP CETN2), we traced their presence from e11.5 primordial germ cells (PGCs) through oogenesis and their ultimate dissolution in mature oocytes. We show tightly coupled CETN2 doublets in PGCs, oogonia, and pre-pubertal oocytes. Beginning with follicular recruitment of incompetent germinal vesicle (GV) oocytes, through full oocyte maturation, the CETN2 doublets separate within the pericentriolar material (PCM) and a rise in single CETN2 pairs is identified, mostly at meiotic metaphase-I and -II spindle poles. Partial CETN2 foci dissolution occurs even as Cep135, a protein necessary for centriole duplication, remains at the PCM. Live imaging demonstrates that the two centrioles break linkage as meiosis resumes and the progressive loss of centriole PCM association. Microtubule inhibition shows that centriole dissolution is uncoupled from microtubule dynamics. Thus, centriole doublets, present in G2-arrested meiotic prophase oocytes, begin partial reduction during follicular recruitment and meiotic resumption, later than previously thought.

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Posted June 05, 2018.
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Separation and Loss of Centrioles from Primordidal Germ Cells to Mature Oocytes in the Mouse
Gerald Schatten, Calvin R Simerly, Marion Manil-Ségalen, Carlos Castro, Carrie Hartnett, Dong Kong, Marie-Helene Verlhac, Jadranka Loncarek
bioRxiv 339572; doi: https://doi.org/10.1101/339572
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Separation and Loss of Centrioles from Primordidal Germ Cells to Mature Oocytes in the Mouse
Gerald Schatten, Calvin R Simerly, Marion Manil-Ségalen, Carlos Castro, Carrie Hartnett, Dong Kong, Marie-Helene Verlhac, Jadranka Loncarek
bioRxiv 339572; doi: https://doi.org/10.1101/339572

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