Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Propofol inhibits endogenous formyl peptide-induced neutrophil activation and alleviates lung injury

Chun-Yu Chen, Yung-Fong Tsai, Wei-Ju Huang, Shih-Hsin Chang, View ORCID ProfileTsong-Long Hwang
doi: https://doi.org/10.1101/340711
Chun-Yu Chen
1Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
2Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yung-Fong Tsai
1Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
2Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wei-Ju Huang
1Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shih-Hsin Chang
1Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
4Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tsong-Long Hwang
1Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan
2Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan
3Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan 333, Taiwan
4Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan
5Department of Chemical Engineering, Ming Chi University of Technology, New Taipei City 243, Taiwan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tsong-Long Hwang
  • For correspondence: htl@mail.cgu.edu.tw
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Critically ill patients have a high risk of sepsis. Various studies have demonstrated that propofol has anti-inflammatory effects that may benefit critically ill patients who require anesthesia. However, the mechanism and therapeutic effect remain incompletely understood. Our previous data suggest that propofol can act as a formyl peptide receptor 1 (FPR1) antagonist. Here, we hypothesize that propofol mitigates sepsis-induced acute lung injury (ALI) by inhibiting mitochondria-derived N-formyl peptide-mediated neutrophil activation. In human neutrophils, propofol competitively reduced the release of elastase, superoxide, and reactive oxygen species induced by fMMYALF, a human mitochondria-derived N-formyl peptide. In addition, propofol significantly inhibited fMMYALF-induced chemotaxis, calcium mobilization, and phosphorylation of protein kinase B and mitogen-activated protein kinases. These results indicate that propofol suppresses neutrophil activation by blocking the interaction between endogenous N-formyl peptide and its receptor, FPR1, thus inhibiting downstream signaling. Furthermore, propofol alleviated alveolar wall disruption, edematous changes, and neutrophil infiltration in lipopolysaccharide-induced ALI in mice. Noticeably, propofol improved the survival of sepsis mice. This study indicates that the anti-neutrophil effects of propofol may benefit critically ill septic patients.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted June 06, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Propofol inhibits endogenous formyl peptide-induced neutrophil activation and alleviates lung injury
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Propofol inhibits endogenous formyl peptide-induced neutrophil activation and alleviates lung injury
Chun-Yu Chen, Yung-Fong Tsai, Wei-Ju Huang, Shih-Hsin Chang, Tsong-Long Hwang
bioRxiv 340711; doi: https://doi.org/10.1101/340711
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Propofol inhibits endogenous formyl peptide-induced neutrophil activation and alleviates lung injury
Chun-Yu Chen, Yung-Fong Tsai, Wei-Ju Huang, Shih-Hsin Chang, Tsong-Long Hwang
bioRxiv 340711; doi: https://doi.org/10.1101/340711

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Pharmacology and Toxicology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3686)
  • Biochemistry (7767)
  • Bioengineering (5666)
  • Bioinformatics (21237)
  • Biophysics (10553)
  • Cancer Biology (8159)
  • Cell Biology (11905)
  • Clinical Trials (138)
  • Developmental Biology (6737)
  • Ecology (10388)
  • Epidemiology (2065)
  • Evolutionary Biology (13838)
  • Genetics (9694)
  • Genomics (13054)
  • Immunology (8121)
  • Microbiology (19936)
  • Molecular Biology (7825)
  • Neuroscience (42959)
  • Paleontology (318)
  • Pathology (1276)
  • Pharmacology and Toxicology (2256)
  • Physiology (3350)
  • Plant Biology (7207)
  • Scientific Communication and Education (1309)
  • Synthetic Biology (1998)
  • Systems Biology (5528)
  • Zoology (1126)