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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

View ORCID ProfileDavid JHF Knapp, Yale S Michaels, Max Jamilly, Quentin RV Ferry, Hector Barbosa, Thomas A Milne, Tudor A Fulga
doi: https://doi.org/10.1101/342485
David JHF Knapp
1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom
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  • ORCID record for David JHF Knapp
  • For correspondence: david.knapp@ndcls.ox.ac.uk tudor.fulga@imm.ox.ac.uk
Yale S Michaels
1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom
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Max Jamilly
1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom
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Quentin RV Ferry
1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom
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Hector Barbosa
1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom
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Thomas A Milne
2Weatherall Institute of Molecular Medicine, MRC Molecular Haematology Unit, NIHR Oxford Biomedical Research Centre Programme, University of Oxford, Oxford OX3 9DS, UK.
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Tudor A Fulga
1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom
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  • For correspondence: david.knapp@ndcls.ox.ac.uk tudor.fulga@imm.ox.ac.uk
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Posted June 08, 2018.
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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
David JHF Knapp, Yale S Michaels, Max Jamilly, Quentin RV Ferry, Hector Barbosa, Thomas A Milne, Tudor A Fulga
bioRxiv 342485; doi: https://doi.org/10.1101/342485
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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
David JHF Knapp, Yale S Michaels, Max Jamilly, Quentin RV Ferry, Hector Barbosa, Thomas A Milne, Tudor A Fulga
bioRxiv 342485; doi: https://doi.org/10.1101/342485

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