Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

David JHF Knapp; Yale S Michaels; Max Jamilly; Quentin RV Ferry; Hector Barbosa; Thomas A Milne; Tudor A Fulga

doi:10.1101/342485

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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

View ORCID ProfileDavid JHF Knapp, Yale S Michaels, Max Jamilly, Quentin RV Ferry, Hector Barbosa, Thomas A Milne, Tudor A Fulga

doi: https://doi.org/10.1101/342485

David JHF Knapp

1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom

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ORCID record for David JHF Knapp
For correspondence: david.knapp@ndcls.ox.ac.uk tudor.fulga@imm.ox.ac.uk

Yale S Michaels

1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom

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Max Jamilly

1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom

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Quentin RV Ferry

1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom

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Hector Barbosa

1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom

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Thomas A Milne

2Weatherall Institute of Molecular Medicine, MRC Molecular Haematology Unit, NIHR Oxford Biomedical Research Centre Programme, University of Oxford, Oxford OX3 9DS, UK.

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Tudor A Fulga

1Weatherall Institute of Molecular Medicine, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom

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For correspondence: david.knapp@ndcls.ox.ac.uk tudor.fulga@imm.ox.ac.uk

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Posted June 08, 2018.

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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

David JHF Knapp, Yale S Michaels, Max Jamilly, Quentin RV Ferry, Hector Barbosa, Thomas A Milne, Tudor A Fulga

bioRxiv 342485; doi: https://doi.org/10.1101/342485

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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

David JHF Knapp, Yale S Michaels, Max Jamilly, Quentin RV Ferry, Hector Barbosa, Thomas A Milne, Tudor A Fulga

bioRxiv 342485; doi: https://doi.org/10.1101/342485

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