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Analysis of the role of Nidogen/entactin in basement membrane assembly and morphogenesis in Drosophila

View ORCID ProfileJianli Dai, View ORCID ProfileBeatriz Estrada, View ORCID ProfileSofie Jacobs, View ORCID ProfileBesaiz J. Sánchez-Sánchez, Jia Tang, Mengqi Ma, View ORCID ProfilePatricia Magadan, View ORCID ProfileJosé C. Pastor-Pareja, View ORCID ProfileMaría D. Martín-Bermudo
doi: https://doi.org/10.1101/346270
Jianli Dai
1School of Life Sciences, Tsinghua University, Beijing 100084, China
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Beatriz Estrada
2CABD (CSIC-Universidad Pablo de Olavide-JA), Seville 41013, Spain
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  • ORCID record for Beatriz Estrada
Sofie Jacobs
1School of Life Sciences, Tsinghua University, Beijing 100084, China
3Rega Institute, KU Leuven, Leuven 3000, Belgium
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Besaiz J. Sánchez-Sánchez
2CABD (CSIC-Universidad Pablo de Olavide-JA), Seville 41013, Spain
4Randall Centre for Cell and Molecular Biophysics, King’s College London, London SE5 9AP, UK
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Jia Tang
1School of Life Sciences, Tsinghua University, Beijing 100084, China
5School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry Univesity, Hangzhou 311300, China
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Mengqi Ma
1School of Life Sciences, Tsinghua University, Beijing 100084, China
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Patricia Magadan
2CABD (CSIC-Universidad Pablo de Olavide-JA), Seville 41013, Spain
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José C. Pastor-Pareja
1School of Life Sciences, Tsinghua University, Beijing 100084, China
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  • For correspondence: mdmarber@upo.es jose.pastor@biomed.tsinghua.edu.cn
María D. Martín-Bermudo
2CABD (CSIC-Universidad Pablo de Olavide-JA), Seville 41013, Spain
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  • For correspondence: mdmarber@upo.es jose.pastor@biomed.tsinghua.edu.cn
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Abstract

Basement membranes (BMs) are thin sheet-like specialized extracellular matrices found at the basal surface of epithelia and endothelial tissues. They have been conserved across evolution and are required for proper tissue growth, organization, differentiation and maintenance. The major constituents of BMs are two independent networks of Laminin and Type IV Collagen interlinked by the proteoglycan Perlecan and the glycoprotein Nidogen/entactin (Ndg). The ability of Ndg to bind in vitro Collagen IV and Laminin, both with key functions during embryogenesis, anticipated an essential role for Ndg on morphogenesis linking the Laminin and Collagen IV networks. This was supported by results from in vitro and cultured embryonic tissues experiments. However, the fact that elimination of Ndg in C. elegans and mice did not affect survival, strongly questioned this proposed linking role. Here, we have isolated mutations in the only Ndg gene present in Drosophila. We find that while, similar to C.elegans and mice, Ndg is not essential for overall organogenesis or viability, it is required for appropriate fertility. We also find, alike in mice, tissue-specific requirements of Ndg for proper assembly and maintenance of certain BMs, namely those of the adipose tissue and flight muscles. In addition, we have performed a thorough functional analysis of the different Ndg domains in vivo. Our results support an essential requirement of the G3 domain for Ndg function and unravel a new key role for the Rod domain in regulating Ndg incorporation into BMs. Furthermore, uncoupling of the Laminin and Collagen IV networks is clearly observed in the larval adipose tissue in the absence of Ndg, indeed supporting a linking role. In light of our findings, we propose that BM assembly and/or maintenance is tissue-specific, which could explain the diverse requirements of a ubiquitous conserved BM component like Nidogen.

Author Summary Basement membranes (BMs) are thin layers of specialized extracellular matrices present in every tissue of the human body. Its main constituents are two networks of Laminin and Type IV Collagen linked by Nidogen (Ndg) and proteoglycans. They form an organized scaffold that regulates organ morphogenesis and function. Mutations affecting BM components are associated with organ dysfunction and several congenital diseases. Thus, a better comprehension of BM assembly and maintenance will not only help to learn more about organogenesis but also to a better understanding and, hopefully, treatment of these diseases. Here, we have used Drosophila to analyse the role of Ndg in BM formation in vivo. Elimination of Ndg in worms and mice does not affect survival, strongly questioning its proposed linking role, derived from in vitro experiments. Here, we show that in the fly Ndg is dispensable for BM assembly and preservation in many tissues, but absolutely required in others. Furthermore, our functional study of the different Ndg domains challenges the significance of some interactions between BM components derived from in vitro experiments, while confirming others, and reveals a new key requirement for the Rod domain in Ndg function and incorporation into BMs.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted June 13, 2018.
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Analysis of the role of Nidogen/entactin in basement membrane assembly and morphogenesis in Drosophila
Jianli Dai, Beatriz Estrada, Sofie Jacobs, Besaiz J. Sánchez-Sánchez, Jia Tang, Mengqi Ma, Patricia Magadan, José C. Pastor-Pareja, María D. Martín-Bermudo
bioRxiv 346270; doi: https://doi.org/10.1101/346270
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Analysis of the role of Nidogen/entactin in basement membrane assembly and morphogenesis in Drosophila
Jianli Dai, Beatriz Estrada, Sofie Jacobs, Besaiz J. Sánchez-Sánchez, Jia Tang, Mengqi Ma, Patricia Magadan, José C. Pastor-Pareja, María D. Martín-Bermudo
bioRxiv 346270; doi: https://doi.org/10.1101/346270

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