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TTL proteins scaffold brassinosteroid signaling components at the plasma membrane to optimize signal transduction in plant cells

View ORCID ProfileVítor Amorim-Silva, Álvaro García-Moreno, Araceli G. Castillo, Naoufal Lakhssassi, Jessica Pérez-Sancho, Yansha Li, Alicia Esteban del Valle, David Posé, Josefa Pérez-Rodriguez, Jinxing Lin, Victoriano Valpuesta, Omar Borsani, Cyril Zipfel, Alberto P. Macho, Miguel A. Botella
doi: https://doi.org/10.1101/351056
Vítor Amorim-Silva
1Departamento de Biología Molecular y Bioquímica. Instituto de Hortofruticultura Subtropical y Mediterranea “La Mayora”, Universidad de Malaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain
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  • ORCID record for Vítor Amorim-Silva
Álvaro García-Moreno
1Departamento de Biología Molecular y Bioquímica. Instituto de Hortofruticultura Subtropical y Mediterranea “La Mayora”, Universidad de Malaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain
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Araceli G. Castillo
2Departamento de Biología Celular, Genética y Fisiología. Instituto de Hortofruticultura Subtropical y Mediterranea “La Mayora”, Universidad de Malaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain
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Naoufal Lakhssassi
3Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA.
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Jessica Pérez-Sancho
1Departamento de Biología Molecular y Bioquímica. Instituto de Hortofruticultura Subtropical y Mediterranea “La Mayora”, Universidad de Malaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain
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Yansha Li
4Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), Shanghai, China
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Alicia Esteban del Valle
1Departamento de Biología Molecular y Bioquímica. Instituto de Hortofruticultura Subtropical y Mediterranea “La Mayora”, Universidad de Malaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain
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David Posé
1Departamento de Biología Molecular y Bioquímica. Instituto de Hortofruticultura Subtropical y Mediterranea “La Mayora”, Universidad de Malaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain
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Josefa Pérez-Rodriguez
1Departamento de Biología Molecular y Bioquímica. Instituto de Hortofruticultura Subtropical y Mediterranea “La Mayora”, Universidad de Malaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain
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Jinxing Lin
5College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
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Victoriano Valpuesta
1Departamento de Biología Molecular y Bioquímica. Instituto de Hortofruticultura Subtropical y Mediterranea “La Mayora”, Universidad de Malaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain
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Omar Borsani
6Departamento de Biología Vegetal, Laboratorio de Bioquímica, Facultad de Agronomía, Montevideo, Uruguay
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Cyril Zipfel
7The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, United Kingdom
8Institute of Plant and Microbial Biology, Zurich-Basel Plant Science Center, University of Zurich, CH-8008 Zurich, Switzerland.
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Alberto P. Macho
4Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), Shanghai, China
7The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, United Kingdom
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Miguel A. Botella
1Departamento de Biología Molecular y Bioquímica. Instituto de Hortofruticultura Subtropical y Mediterranea “La Mayora”, Universidad de Malaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, Campus Teatinos, 29071 Málaga, Spain
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  • For correspondence: mabotella@uma.es
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Abstract

Brassinosteroids (BRs) form a group of steroidal hormones essential for plant growth, development and stress responses. Here, we report that plant-specific TETRATRICOPEPTIDE THIOREDOXIN-LIKE (TTL) proteins are positive regulators of BR signaling functioning as scaffold for BR signaling components in Arabidopsis. TTL3 forms a complex with all core components involved in BR signaling, including the receptor kinase BRASSINOSTEROID INSENSITIVE1 (BRI1), the transcription factor BRASSINAZOLE RESISTANT1 (BZR1) and the phosphatase BRI1-SUPPRESSOR1 (BSU1), but excluding the co-receptor BAK1. TTL3 is mainly localized in the cytoplasm, but BR treatment increases its localization at the plasma membrane, where it strengthens the association with BR signaling components. Consistent with a role in BR signaling, mutations in TTL3 and related TTL1 and TTL4 genes cause reduced BR responsiveness. We propose a mechanistic model for BR signaling, in which cytoplasmic/nuclear BR components bound to TTL proteins are recruited to the plasma membrane upon BR perception, which in turn allows the assembly of a BR signaling complex, leading to the de-phosphorylation and nuclear accumulation of the transcription factors BZR1 and BES1.

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Posted June 20, 2018.
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TTL proteins scaffold brassinosteroid signaling components at the plasma membrane to optimize signal transduction in plant cells
Vítor Amorim-Silva, Álvaro García-Moreno, Araceli G. Castillo, Naoufal Lakhssassi, Jessica Pérez-Sancho, Yansha Li, Alicia Esteban del Valle, David Posé, Josefa Pérez-Rodriguez, Jinxing Lin, Victoriano Valpuesta, Omar Borsani, Cyril Zipfel, Alberto P. Macho, Miguel A. Botella
bioRxiv 351056; doi: https://doi.org/10.1101/351056
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TTL proteins scaffold brassinosteroid signaling components at the plasma membrane to optimize signal transduction in plant cells
Vítor Amorim-Silva, Álvaro García-Moreno, Araceli G. Castillo, Naoufal Lakhssassi, Jessica Pérez-Sancho, Yansha Li, Alicia Esteban del Valle, David Posé, Josefa Pérez-Rodriguez, Jinxing Lin, Victoriano Valpuesta, Omar Borsani, Cyril Zipfel, Alberto P. Macho, Miguel A. Botella
bioRxiv 351056; doi: https://doi.org/10.1101/351056

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