Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR

Gemma Danks, Heloisa Galbiati, Martina Raasholm, Yamila N. Torres Cleuren, Eivind Valen, Pavla Navratilova, Eric M. Thompson
doi: https://doi.org/10.1101/353979
Gemma Danks
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Heloisa Galbiati
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Martina Raasholm
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yamila N. Torres Cleuren
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eivind Valen
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pavla Navratilova
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric M. Thompson
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

In phylogenetically diverse organisms, the 5’ ends of a subset of mRNAs are trans-spliced with a spliced leader (SL) RNA. The functions of SL trans-splicing, however, remain largely enigmatic. Here, we quantified translation genome-wide in the marine chordate, Oikopleura dioica, under inhibition of mTOR, a central growth regulator. Translation of trans-spliced TOP mRNAs was suppressed, showing that the SL sequence permits nutrient-dependent translational control of growth-related mRNAs. Under crowded, nutrient-limiting conditions, O. dioica continues to filter-feed, but arrests growth until favorable conditions return. Upon release from such conditions, initial recovery was independent of nutrient-responsive, trans-spliced genes, suggesting animal density sensing as a first trigger for resumption of development. Our results demonstrate a role for trans-splicing in the coordinated translational down-regulation of nutrient-responsive genes under limiting conditions and suggest an innovative strategy for rapid evolution of mTOR targets in genomes of metazoans whose reproduction is tightly linked to nutritional cues.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted June 22, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR
Gemma Danks, Heloisa Galbiati, Martina Raasholm, Yamila N. Torres Cleuren, Eivind Valen, Pavla Navratilova, Eric M. Thompson
bioRxiv 353979; doi: https://doi.org/10.1101/353979
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR
Gemma Danks, Heloisa Galbiati, Martina Raasholm, Yamila N. Torres Cleuren, Eivind Valen, Pavla Navratilova, Eric M. Thompson
bioRxiv 353979; doi: https://doi.org/10.1101/353979

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3502)
  • Biochemistry (7343)
  • Bioengineering (5319)
  • Bioinformatics (20258)
  • Biophysics (10008)
  • Cancer Biology (7735)
  • Cell Biology (11293)
  • Clinical Trials (138)
  • Developmental Biology (6434)
  • Ecology (9947)
  • Epidemiology (2065)
  • Evolutionary Biology (13315)
  • Genetics (9359)
  • Genomics (12579)
  • Immunology (7696)
  • Microbiology (19008)
  • Molecular Biology (7437)
  • Neuroscience (41011)
  • Paleontology (300)
  • Pathology (1228)
  • Pharmacology and Toxicology (2134)
  • Physiology (3155)
  • Plant Biology (6858)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1895)
  • Systems Biology (5311)
  • Zoology (1087)