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Vimentin filaments interact with the mitotic cortex allowing normal cell division

Sofia Duarte, Álvaro Viedma-Poyatos, Elena Navarro-Carrasco, Alma E. Martínez, María A. Pajares, Dolores Pérez-Sala
doi: https://doi.org/10.1101/356642
Sofia Duarte
1Department of Structural and Chemical Biology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28029 Madrid, Spain.
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Álvaro Viedma-Poyatos
1Department of Structural and Chemical Biology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28029 Madrid, Spain.
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Elena Navarro-Carrasco
1Department of Structural and Chemical Biology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28029 Madrid, Spain.
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Alma E. Martínez
1Department of Structural and Chemical Biology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28029 Madrid, Spain.
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María A. Pajares
1Department of Structural and Chemical Biology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28029 Madrid, Spain.
2Molecular Hepatology Group, Instituto de Investigación Sanitaria La Paz (IdiPAZ), Paseo de la Castellana 261, 28046 Madrid, Spain.
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Dolores Pérez-Sala
1Department of Structural and Chemical Biology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28029 Madrid, Spain.
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  • For correspondence: dperezsala@cib.csic.es
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Abstract

The vimentin network displays remarkable plasticity to support basic cellular functions. Here, we show that in several cell types vimentin filaments redistribute to the cell periphery during mitosis, forming a robust scaffold interwoven with cortical actin. Importantly, the intrinsically disordered tail domain of vimentin is essential for this redistribution, which allows normal mitotic progression. A tailless vimentin mutant forms curly bundles, which remain entangled with dividing chromosomes in mitosis leading to mitotic catastrophes or asymmetric partitions. Serial deletions of the tail domain induce an increasing impairment of cortical association and mitosis progression. Disruption of actin, but not microtubules, mimics the impact of tail deletion. Pathophysiological stimuli, including HIV-protease and lipoxidation, induce similar alterations. Interestingly, filament integrity is not necessary for cortical association, which also occurs in vimentin particles. Taken together, these results unveil novel implications of vimentin dynamics in cell division, in which the tail domain plays a key role.

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Posted June 27, 2018.
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Vimentin filaments interact with the mitotic cortex allowing normal cell division
Sofia Duarte, Álvaro Viedma-Poyatos, Elena Navarro-Carrasco, Alma E. Martínez, María A. Pajares, Dolores Pérez-Sala
bioRxiv 356642; doi: https://doi.org/10.1101/356642
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Vimentin filaments interact with the mitotic cortex allowing normal cell division
Sofia Duarte, Álvaro Viedma-Poyatos, Elena Navarro-Carrasco, Alma E. Martínez, María A. Pajares, Dolores Pérez-Sala
bioRxiv 356642; doi: https://doi.org/10.1101/356642

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