Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Cryo-EM structure of an amyloid fibril from systemic amyloidosis

View ORCID ProfileFalk Liberta, Sarah Loerch, Matthies Rennegarbe, Angelika Schierhorn, Per Westermark, Gunilla T. Westermark, Nikolaus Grigorieff, View ORCID ProfileMarcus Fändrich, Matthias Schmidt
doi: https://doi.org/10.1101/357129
Falk Liberta
1Institute of Protein Biochemistry, Ulm University, 89081 Ulm, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Falk Liberta
Sarah Loerch
2Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matthies Rennegarbe
1Institute of Protein Biochemistry, Ulm University, 89081 Ulm, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Angelika Schierhorn
3Institute of Biochemistry and Biotechnology, Martin-Luther-University, 06120 Halle (Saale), Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Per Westermark
4Department of Immunology, Genetics, and Pathology, Uppsala University, Uppsala SE-751 85 Sweden.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gunilla T. Westermark
5Department of Medical Cell Biology, Uppsala University, SE-75123 Uppsala, Sweden.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nikolaus Grigorieff
2Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marcus Fändrich
1Institute of Protein Biochemistry, Ulm University, 89081 Ulm, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Marcus Fändrich
  • For correspondence: marcus.faendrich@uni-ulm.de matthias.schmidt@uni-ulm.de
Matthias Schmidt
1Institute of Protein Biochemistry, Ulm University, 89081 Ulm, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: marcus.faendrich@uni-ulm.de matthias.schmidt@uni-ulm.de
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Systemic AA amyloidosis is a worldwide occurring disease of humans and animals that arises from the misfolding of serum amyloid A protein. To provide insights into the molecular basis of this disease we used electron cryo-microscopy and determined the structure of an ex vivo amyloid fibril purified from AA amyloidotic mice at 3.0 Å resolution. The fibril consists of C-terminally truncated serum amyloid A protein arranged into a compactly folded all-β conformation. The structure identifies the protein N-terminus as central for the assembly of this fibril and provides a mechanism for its prion-like replication. Our data further explain how amino acid substitutions within the tightly packed fibril core can lead to amyloid resistance in vivo.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted June 29, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Cryo-EM structure of an amyloid fibril from systemic amyloidosis
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Cryo-EM structure of an amyloid fibril from systemic amyloidosis
Falk Liberta, Sarah Loerch, Matthies Rennegarbe, Angelika Schierhorn, Per Westermark, Gunilla T. Westermark, Nikolaus Grigorieff, Marcus Fändrich, Matthias Schmidt
bioRxiv 357129; doi: https://doi.org/10.1101/357129
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Cryo-EM structure of an amyloid fibril from systemic amyloidosis
Falk Liberta, Sarah Loerch, Matthies Rennegarbe, Angelika Schierhorn, Per Westermark, Gunilla T. Westermark, Nikolaus Grigorieff, Marcus Fändrich, Matthias Schmidt
bioRxiv 357129; doi: https://doi.org/10.1101/357129

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4378)
  • Biochemistry (9571)
  • Bioengineering (7082)
  • Bioinformatics (24821)
  • Biophysics (12595)
  • Cancer Biology (9944)
  • Cell Biology (14333)
  • Clinical Trials (138)
  • Developmental Biology (7942)
  • Ecology (12092)
  • Epidemiology (2067)
  • Evolutionary Biology (15979)
  • Genetics (10914)
  • Genomics (14726)
  • Immunology (9859)
  • Microbiology (23633)
  • Molecular Biology (9472)
  • Neuroscience (50815)
  • Paleontology (369)
  • Pathology (1538)
  • Pharmacology and Toxicology (2677)
  • Physiology (4005)
  • Plant Biology (8651)
  • Scientific Communication and Education (1508)
  • Synthetic Biology (2389)
  • Systems Biology (6420)
  • Zoology (1345)