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Mechanosensitive binding of p120-Catenin at cell junctions regulates E-Cadherin turnover and epithelial viscoelasticity

K. Venkatesan Iyer, Romina Piscitello-Gómez, Frank Jülicher, Suzanne Eaton
doi: https://doi.org/10.1101/357186
K. Venkatesan Iyer
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden, Germany
2Max Planck Institute of Physics of Complex Systems, Nöthnitzer Strasse 38, Dresden, Germany
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  • For correspondence: eaton@mpi-cbg.de julicher@pks.mpg.de iyer@mpi-cbg.de
Romina Piscitello-Gómez
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden, Germany
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Frank Jülicher
2Max Planck Institute of Physics of Complex Systems, Nöthnitzer Strasse 38, Dresden, Germany
4Center for Systems Biology Dresden, Pfotenhauerstrasse 108, Dresden
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  • For correspondence: eaton@mpi-cbg.de julicher@pks.mpg.de iyer@mpi-cbg.de
Suzanne Eaton
1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden, Germany
3Biotechnologisches Zentrum, Technische Universität Dresden, Tatzberg 47/49 Dresden, Germany
4Center for Systems Biology Dresden, Pfotenhauerstrasse 108, Dresden
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  • For correspondence: eaton@mpi-cbg.de julicher@pks.mpg.de iyer@mpi-cbg.de
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Abstract

Studying how epithelia respond to mechanical stresses is key to understanding tissue shape changes during morphogenesis. Here, we study the viscoelastic deformation of the Drosophila pupal wing epithelium in response to mechanical stress that evolves during morphogenesis. We show that wing epithelial tissue viscoelasticity depends on endocytic turnover of E-Cadherin. The fraction of ECadherin undergoing turnover depends on mechanical stress in the epithelium. We identified mechanosensitive binding of the endocytic regulator p120-Catenin (p120) as a mechanism to regulate E-Cadherin turnover. Under high stress, p120 is released into the cytoplasm, destabilizing E-Cadherin complexes and increasing its turnover. In p120 mutants, E-Cadherin turnover is insensitive to mechanical stress. Furthermore, we show that p120 is crucial for the viscoelastic deformation of the wing epithelium. Taken together, our findings reveal that mechanosensitive binding of p120-Catenin tunes epithelial tissue viscoelasticity during morphogenesis.

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Posted June 27, 2018.
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Mechanosensitive binding of p120-Catenin at cell junctions regulates E-Cadherin turnover and epithelial viscoelasticity
K. Venkatesan Iyer, Romina Piscitello-Gómez, Frank Jülicher, Suzanne Eaton
bioRxiv 357186; doi: https://doi.org/10.1101/357186
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Mechanosensitive binding of p120-Catenin at cell junctions regulates E-Cadherin turnover and epithelial viscoelasticity
K. Venkatesan Iyer, Romina Piscitello-Gómez, Frank Jülicher, Suzanne Eaton
bioRxiv 357186; doi: https://doi.org/10.1101/357186

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