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Far beyond common leeching: insights into an ancient medical device through integrated omics data

View ORCID ProfileVladislav V. Babenko, Oleg V. Podgorny, Valentin A. Manuvera, Artem S. Kasianov, Alexander I. Manolov, Ekaterina N. Grafskaia, Dmitriy A. Shirokov, Alexey S. Kurdyumov, Dmitriy V. Vinogradov, Anastasia S. Nikitina, Sergey I. Kovalchuk, Nickolay A. Anikanov, Ivan O. Butenko, Maria T. Vakhitova, Olga V. Pobeguts, Daria S. Matushkina, Daria V. Rakitina, Daria D. Kharlampieva, Nadezhda F. Polina, Pavel A. Bobrovsky, Elena S. Kostryukova, Victor G. Zgoda, Isolda P. Baskova, Mikhail S. Gelfand, Vadim M. Govorun, Vassili N. Lazarev
doi: https://doi.org/10.1101/357681
Vladislav V. Babenko
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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  • ORCID record for Vladislav V. Babenko
  • For correspondence: daniorerio34@gmail.com
Oleg V. Podgorny
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
2Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov str., Moscow 119334, Russia
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Valentin A. Manuvera
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
3Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
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Artem S. Kasianov
3Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
4Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina str., Moscow 119991, Russia
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Alexander I. Manolov
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Ekaterina N. Grafskaia
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
3Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
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Dmitriy A. Shirokov
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Alexey S. Kurdyumov
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Dmitriy V. Vinogradov
5A.A.Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, 19 Bol’shoi Karetnyi per., Moscow 127051, Russia
6Skolkovo Institute of Science and Technology, 3 Nobelya Ulitsa str., Moscow 121205, Russia
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Anastasia S. Nikitina
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Sergey I. Kovalchuk
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
7Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str., Moscow 117997, Russia
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Nickolay A. Anikanov
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
7Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str., Moscow 117997, Russia
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Ivan O. Butenko
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Maria T. Vakhitova
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
3Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
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Olga V. Pobeguts
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Daria S. Matushkina
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Daria V. Rakitina
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Daria D. Kharlampieva
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Nadezhda F. Polina
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Pavel A. Bobrovsky
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Elena S. Kostryukova
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
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Victor G. Zgoda
8V.N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, 10 Pogodinskaja str., Moscow 119832, Russia
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Isolda P. Baskova
9Faculty of Biology, Lomonosov Moscow State University, 1–12 Leninskie Gory, Moscow 119991, Russia.
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Mikhail S. Gelfand
5A.A.Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, 19 Bol’shoi Karetnyi per., Moscow 127051, Russia
6Skolkovo Institute of Science and Technology, 3 Nobelya Ulitsa str., Moscow 121205, Russia
10Faculty of Computer Science, National Research University Higher School of Economics, 20 Myasnitskaya str., Moscow 101000, Russia
11Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 1–73 Leninskie Gory, Moscow 119991, Russia
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Vadim M. Govorun
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
3Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
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Vassili N. Lazarev
1Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str., Moscow 119435, Russia
3Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russia
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Abstract

Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. To fill the gap in our knowledge about the genetically encoded components of leech SCS, we annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, Hirudo orientalis, and Hirudo verbana. Differential expression analysis verified by proteomics identified salivary cell-specific genes, many of which encode previously unknown salivary components. However, the genes encoding known anticoagulants were not differentially expressed in the salivary cells. The function-related analysis of the unique salivary cell genes enabled an update of the concept of interactions between salivary proteins and components of haemostasis. In addition, a metagenomic analysis performed in our study revealed the overall metabolic potential of the leech microbiota. Thus, our study extends the knowledge of the genetic fundamentals of the blood-sucking lifestyle in leeches.

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Posted June 28, 2018.
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Far beyond common leeching: insights into an ancient medical device through integrated omics data
Vladislav V. Babenko, Oleg V. Podgorny, Valentin A. Manuvera, Artem S. Kasianov, Alexander I. Manolov, Ekaterina N. Grafskaia, Dmitriy A. Shirokov, Alexey S. Kurdyumov, Dmitriy V. Vinogradov, Anastasia S. Nikitina, Sergey I. Kovalchuk, Nickolay A. Anikanov, Ivan O. Butenko, Maria T. Vakhitova, Olga V. Pobeguts, Daria S. Matushkina, Daria V. Rakitina, Daria D. Kharlampieva, Nadezhda F. Polina, Pavel A. Bobrovsky, Elena S. Kostryukova, Victor G. Zgoda, Isolda P. Baskova, Mikhail S. Gelfand, Vadim M. Govorun, Vassili N. Lazarev
bioRxiv 357681; doi: https://doi.org/10.1101/357681
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Far beyond common leeching: insights into an ancient medical device through integrated omics data
Vladislav V. Babenko, Oleg V. Podgorny, Valentin A. Manuvera, Artem S. Kasianov, Alexander I. Manolov, Ekaterina N. Grafskaia, Dmitriy A. Shirokov, Alexey S. Kurdyumov, Dmitriy V. Vinogradov, Anastasia S. Nikitina, Sergey I. Kovalchuk, Nickolay A. Anikanov, Ivan O. Butenko, Maria T. Vakhitova, Olga V. Pobeguts, Daria S. Matushkina, Daria V. Rakitina, Daria D. Kharlampieva, Nadezhda F. Polina, Pavel A. Bobrovsky, Elena S. Kostryukova, Victor G. Zgoda, Isolda P. Baskova, Mikhail S. Gelfand, Vadim M. Govorun, Vassili N. Lazarev
bioRxiv 357681; doi: https://doi.org/10.1101/357681

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