Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Tissue embedding in a silica hydrogel for functional investigations

View ORCID ProfileFabio Miazzi, Sabine Kaltofen, Jan E. Bello, Bill S. Hansson, View ORCID ProfileDieter Wicher
doi: https://doi.org/10.1101/357962
Fabio Miazzi
Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell Str. 8, D-07745 Jena, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Fabio Miazzi
Sabine Kaltofen
Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell Str. 8, D-07745 Jena, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jan E. Bello
Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell Str. 8, D-07745 Jena, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bill S. Hansson
Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell Str. 8, D-07745 Jena, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dieter Wicher
Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell Str. 8, D-07745 Jena, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Dieter Wicher
  • For correspondence: dwicher@ice.mpg.de
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Embedding procedures can be challenging for delicate specimens. We present a method based on a sodium metasilicate (waterglass) gel to embed tissue samples for acute physiological studies. We show that the application of such a colloidal gel has minimal effects on the properties of buffered solutions and cell activity, allowing functional investigations on sensitive cells such as ciliated insect olfactory neurons.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted June 28, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Tissue embedding in a silica hydrogel for functional investigations
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Tissue embedding in a silica hydrogel for functional investigations
Fabio Miazzi, Sabine Kaltofen, Jan E. Bello, Bill S. Hansson, Dieter Wicher
bioRxiv 357962; doi: https://doi.org/10.1101/357962
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Tissue embedding in a silica hydrogel for functional investigations
Fabio Miazzi, Sabine Kaltofen, Jan E. Bello, Bill S. Hansson, Dieter Wicher
bioRxiv 357962; doi: https://doi.org/10.1101/357962

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (3502)
  • Biochemistry (7343)
  • Bioengineering (5319)
  • Bioinformatics (20258)
  • Biophysics (10008)
  • Cancer Biology (7735)
  • Cell Biology (11293)
  • Clinical Trials (138)
  • Developmental Biology (6434)
  • Ecology (9947)
  • Epidemiology (2065)
  • Evolutionary Biology (13315)
  • Genetics (9359)
  • Genomics (12579)
  • Immunology (7696)
  • Microbiology (19008)
  • Molecular Biology (7437)
  • Neuroscience (41011)
  • Paleontology (300)
  • Pathology (1228)
  • Pharmacology and Toxicology (2134)
  • Physiology (3155)
  • Plant Biology (6858)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1895)
  • Systems Biology (5311)
  • Zoology (1087)