Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Post-translational modifications drive protein stability to control the dynamic beer brewing proteome

View ORCID ProfileEdward D. Kerr, Christopher H. Caboche, View ORCID ProfileBenjamin L. Schulz
doi: https://doi.org/10.1101/358796
Edward D. Kerr
School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, AustraliaAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Edward D. Kerr
Christopher H. Caboche
School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, AustraliaAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane 4072, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benjamin L. Schulz
School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia 4072, Queensland, AustraliaAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane 4072, AustraliaCentre for Biopharmaceutical Innovation, Australian Institute of Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, Queensland, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Benjamin L. Schulz
  • For correspondence: b.schulz@uq.edu.au
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Mashing is a key step in beer brewing in which starch and proteins are solubilized from malted barley in a hot water extraction and digested to oligomaltose and free amino nitrogen. We used SWATH-MS to measure the abundance and site-specific modifications of proteins throughout a small-scale pale ale mash. Proteins extracted from the malt at low temperatures early in the mash decreased precipitously in abundance at higher temperatures late in the mash due to temperature/time-induced unfolding and aggregation. We validated these observations using experimental manipulation of time and temperature parameters in a micro-scale pale ale mash. Correlation analysis of temperature/time-dependent abundance showed that sequence and structure were the main features that controlled protein abundance profiles. Partial proteolysis by barley proteases was common early in the mash. The resulting proteolytically clipped proteins were particularly sensitive and were preferentially lost at high temperatures late in the mash, while intact proteins remained soluble. The beer brewing proteome is therefore driven by the interplay between protein solubilisation and proteolysis, which are in turn determined by barley variety, growth conditions, and brewing process parameters.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted June 03, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Post-translational modifications drive protein stability to control the dynamic beer brewing proteome
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
Post-translational modifications drive protein stability to control the dynamic beer brewing proteome
Edward D. Kerr, Christopher H. Caboche, Benjamin L. Schulz
bioRxiv 358796; doi: https://doi.org/10.1101/358796
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Post-translational modifications drive protein stability to control the dynamic beer brewing proteome
Edward D. Kerr, Christopher H. Caboche, Benjamin L. Schulz
bioRxiv 358796; doi: https://doi.org/10.1101/358796

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biochemistry
Subject Areas
All Articles
  • Animal Behavior and Cognition (1519)
  • Biochemistry (2473)
  • Bioengineering (1727)
  • Bioinformatics (9648)
  • Biophysics (3884)
  • Cancer Biology (2961)
  • Cell Biology (4173)
  • Clinical Trials (135)
  • Developmental Biology (2620)
  • Ecology (4084)
  • Epidemiology (2031)
  • Evolutionary Biology (6868)
  • Genetics (5195)
  • Genomics (6482)
  • Immunology (2176)
  • Microbiology (6909)
  • Molecular Biology (2746)
  • Neuroscience (17197)
  • Paleontology (125)
  • Pathology (425)
  • Pharmacology and Toxicology (703)
  • Physiology (1050)
  • Plant Biology (2478)
  • Scientific Communication and Education (642)
  • Synthetic Biology (828)
  • Systems Biology (2680)
  • Zoology (429)