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Multiplexed single-cell RNA-seq via transient barcoding for drug screening

Dongju Shin, Wookjae Lee, Ji Hyun Lee, Duhee Bang
doi: https://doi.org/10.1101/359851
Dongju Shin
1Department of Chemistry, Yonsei University, Seoul, Korea
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Wookjae Lee
1Department of Chemistry, Yonsei University, Seoul, Korea
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Ji Hyun Lee
2Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul, Korea
3Department of Biomedical Science and Technology, Kyung Hee Medical Science Research Institute, Kyung Hee University, Seoul, Korea
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Duhee Bang
1Department of Chemistry, Yonsei University, Seoul, Korea
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Abstract

To simultaneously analyze multiple samples of various conditions with scRNA-seq, we developed a universal sample barcoding method through transient transfection of SBOs. A 48-plex drug treatment experiment of pooled samples analyzed by a single run of Drop-Seq revealed a unique transcriptome response for each drug and target-specific gene expression signatures at the single-cell level. Our cost-effective method is widely applicable for single-cell profiling of multiple experimental conditions.

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Posted June 30, 2018.
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Multiplexed single-cell RNA-seq via transient barcoding for drug screening
Dongju Shin, Wookjae Lee, Ji Hyun Lee, Duhee Bang
bioRxiv 359851; doi: https://doi.org/10.1101/359851
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Multiplexed single-cell RNA-seq via transient barcoding for drug screening
Dongju Shin, Wookjae Lee, Ji Hyun Lee, Duhee Bang
bioRxiv 359851; doi: https://doi.org/10.1101/359851

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