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Drosophila kinesin-8 stabilises the kinetochore-microtubule interaction

Tomoya Edzuka, Gohta Goshima
doi: https://doi.org/10.1101/363150
Tomoya Edzuka
1Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan
2Marine Biological Laboratory, Woods Hole, MA 02543, USA
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Gohta Goshima
1Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan
2Marine Biological Laboratory, Woods Hole, MA 02543, USA
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  • For correspondence: goshima@bio.nagoya-u.ac.jp
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Abstract

Kinesin-8 is required for proper chromosome alignment in a variety of animal and yeast cell types. However, how this conserved motor protein controls chromosome alignment remains unclear, as multiple biochemical activities, including inconsistent ones between studies, have been identified for this motor family. Here, we show that Drosophila kinesin-8 Klp67A possesses both microtubule (MT) plus-end-stabilising and ‐destabilising activities in addition to commonly observed MT plus-end-directed motility and tubulin-binding activity in vitro, and is required for stable kinetochore-MT attachment during prometaphase in S2 cells In the absence of kinesin-8Klp67A, abnormally-long MTs interact in an “end-on” fashion with kinetochores at normal frequency. However, the interaction was not stable and, once-attached, MTs were frequently detached. This phenotype was rescued by ectopic expression of MT plus-end-stabilising factor CLASP, but not by artificial shortening of MTs. These results suggest that MT-stabilising activity of kinesin-8Klp67A is critical for stable kinetochore-MT attachment. Finally, human kinesin-8KIF18A was also shown important to ensure proper MT attachment.

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Posted October 25, 2018.
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Drosophila kinesin-8 stabilises the kinetochore-microtubule interaction
Tomoya Edzuka, Gohta Goshima
bioRxiv 363150; doi: https://doi.org/10.1101/363150
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Drosophila kinesin-8 stabilises the kinetochore-microtubule interaction
Tomoya Edzuka, Gohta Goshima
bioRxiv 363150; doi: https://doi.org/10.1101/363150

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