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Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments

View ORCID ProfileAnne-Sophie Hafner, View ORCID ProfilePaul G. Donlin-Asp, View ORCID ProfileBeulah Leitch, View ORCID ProfileEtienne Herzog, View ORCID ProfileErin M. Schuman
doi: https://doi.org/10.1101/363184
Anne-Sophie Hafner
1Max Planck Institute for Brain Research, Frankfurt Germany
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Paul G. Donlin-Asp
1Max Planck Institute for Brain Research, Frankfurt Germany
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Beulah Leitch
2Department of Anatomy, School of Biomedical Sciences and the Brain Health Research Centre, University of Otago, Dunedin, New Zealand
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Etienne Herzog
3Interdisciplinary Institute for Neuroscience, University Bordeaux, UMR 5297, F-33000, Bordeaux, France
4Interdisciplinary Institute for Neuroscience, CNRS, UMR 5297, F-33000, Bordeaux, France
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Erin M. Schuman
1Max Planck Institute for Brain Research, Frankfurt Germany
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  • For correspondence: erin.schuman@brain.mpg.de
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Abstract

There is ample evidence for localized mRNAs and protein synthesis in neuronal dendrites, however, demonstrations of these processes in presynaptic terminals are limited. We used expansion microscopy to resolve pre- and postsynaptic compartments in brain slices. Most presynaptic terminals in the hippocampus and forebrain contained mRNA and ribosomes. We sorted fluorescently labeled synaptosomes from mouse brain and then sequenced hundreds of mRNA species present within excitatory boutons. After brief metabolic labeling, more them 30% of all presynaptic terminals exhibited a signal, providing evidence for ongoing protein synthesis. We tested different classic plasticity paradigms and observed unique patterns of rapid pre- and/or postsynaptic translation. Thus presynaptic terminals are translationally competent and local protein synthesis is differentially recruited to drive compartment-specific phenotypes that underlie different forms of plasticity.

One sentence summary Protein synthesis occurs in all synaptic compartments, including excitatory and inhibitory axon terminals.

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Posted March 26, 2019.
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Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments
Anne-Sophie Hafner, Paul G. Donlin-Asp, Beulah Leitch, Etienne Herzog, Erin M. Schuman
bioRxiv 363184; doi: https://doi.org/10.1101/363184
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Local protein synthesis is a ubiquitous feature of neuronal pre- and postsynaptic compartments
Anne-Sophie Hafner, Paul G. Donlin-Asp, Beulah Leitch, Etienne Herzog, Erin M. Schuman
bioRxiv 363184; doi: https://doi.org/10.1101/363184

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