Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The first genetic linkage map for Fraxinus pennsylvanica and syntenic relationships with four related species

Di Wu, Jennifer Koch, Mark Coggeshall, John Carlson
doi: https://doi.org/10.1101/365676
Di Wu
1Department of Ecosystem Science and Management, Pennsylvania State University, University Park, PA 16802 USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jennifer Koch
2USDA Forest Service, Northern Research Station, Project NRS-16. 359 Main Road, Delaware, OH 43015 USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mark Coggeshall
3Department of Forestry, Center for Agroforestry, University of Missouri, Columbia, MO 65211 USA
4USDA Forest Service, Northern Research Station, Hardwood Tree Improvement and Regeneration Center, Project NRS-14. 715 W. State Street, West Lafayette, IN 47907 USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John Carlson
1Department of Ecosystem Science and Management, Pennsylvania State University, University Park, PA 16802 USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Green ash (Fraxinus pennsylvanica) is an outcrossing, diploid (2n=46) hardwood tree species, native to North America. Native ash species in North America are being threatened by the rapid invasion of emerald ash borer (EAB, Agrilus planipennis) from Asia. Green ash, the most widely distributed ash species, is severely affected by EAB infestation, yet few resources for genetic studies and improvement of green ash are available. In this study, a total of 5,712 high quality single nucleotide polymorphisms (SNPs) were discovered using a minimum allele frequency of 1% across the entire genome through genotyping-by-sequencing. We also screened hundreds of genomic- and EST-based microsatellite markers (SSRs) from previous de novo assemblies (Staton et al. 2015; Lane et al. 2016). A first genetic linkage map of green ash was constructed from 91 individuals in a full-sib family, combining 2,719 SNP and 84 SSR segregating markers among the parental maps. The consensus SNP and SSR map contains a total of 1,201 markers in 23 linkage groups spanning 2008.87cM, at an average inter-marker distance of 1.67 cM with a minimum logarithm of odds (LOD) of 6 and maximum recombination fraction of 0.40. Comparisons of the organization the green ash map with the genomes of asterid species coffee and tomato, and genomes of the rosid species poplar and peach, showed areas of conserved gene order, with overall synteny strongest with coffee.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted July 09, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The first genetic linkage map for Fraxinus pennsylvanica and syntenic relationships with four related species
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The first genetic linkage map for Fraxinus pennsylvanica and syntenic relationships with four related species
Di Wu, Jennifer Koch, Mark Coggeshall, John Carlson
bioRxiv 365676; doi: https://doi.org/10.1101/365676
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
The first genetic linkage map for Fraxinus pennsylvanica and syntenic relationships with four related species
Di Wu, Jennifer Koch, Mark Coggeshall, John Carlson
bioRxiv 365676; doi: https://doi.org/10.1101/365676

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4237)
  • Biochemistry (9147)
  • Bioengineering (6786)
  • Bioinformatics (24020)
  • Biophysics (12137)
  • Cancer Biology (9544)
  • Cell Biology (13795)
  • Clinical Trials (138)
  • Developmental Biology (7642)
  • Ecology (11715)
  • Epidemiology (2066)
  • Evolutionary Biology (15517)
  • Genetics (10650)
  • Genomics (14332)
  • Immunology (9492)
  • Microbiology (22856)
  • Molecular Biology (9103)
  • Neuroscience (49028)
  • Paleontology (355)
  • Pathology (1484)
  • Pharmacology and Toxicology (2572)
  • Physiology (3848)
  • Plant Biology (8337)
  • Scientific Communication and Education (1472)
  • Synthetic Biology (2296)
  • Systems Biology (6196)
  • Zoology (1302)