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CTCF sites display cell cycle dependent dynamics in factor binding and nucleosome positioning

View ORCID ProfileMarlies E. Oomen, View ORCID ProfileAnders S. Hansen, View ORCID ProfileYu Liu, View ORCID ProfileXavier Darzacq, View ORCID ProfileJob Dekker
doi: https://doi.org/10.1101/365866
Marlies E. Oomen
1Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
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Anders S. Hansen
2Department of Molecular and Cell Biology, Li Ka Shing Center for Biomedical and Health Sciences, CIRM Center of Excellence, University of California, Berkeley, CA 94720, USA
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Yu Liu
1Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
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Xavier Darzacq
2Department of Molecular and Cell Biology, Li Ka Shing Center for Biomedical and Health Sciences, CIRM Center of Excellence, University of California, Berkeley, CA 94720, USA
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Job Dekker
1Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA
3Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 208156789, USA
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  • For correspondence: job.dekker@umassmed.edu
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Abstract

CTCF plays a key role in formation of topologically associating domains (TADs) and loops in interphase. During mitosis TADs are absent, but how TAD formation is dynamically controlled during the cell cycle is not known. Several contradicting observations have been made regarding CTCF binding to mitotic chromatin using both genomics and microscopy-based techniques. Here we have used 4 different assays to address this debate. First, using 5C we confirmed that TADs and CTCF loops are readily detected in interphase, but absent during prometaphase. Second, ATAC-seq analysis showed that CTCF sites display greatly reduced accessibility and lose the CTCF footprint in prometaphase, suggesting loss of CTCF binding and rearrangement of the nucleosomal array around the binding motif. In contrast, transcription start sites remain accessible in prometaphase, although adjacent nucleosomes can also become repositioned and occupy at least a subset of start sites during mitosis. Third, loss of site-specific CTCF binding was directly demonstrated using CUT&RUN. Histone modifications and histone variants are maintained in mitosis, suggesting a role in bookmarking of active CTCF sites. Finally, live-cell imaging, fluorescence recovery after photobleaching and single molecule tracking showed that almost all CTCF chromatin binding is lost in prometaphase. Combined, our results demonstrate loss of CTCF binding to CTCF sites during prometaphase and rearrangement of the chromatin landscape around CTCF motifs. This contributes to loss of TADs and CTCF loops during mitosis, and reveals that CTCF sites, a key architectural cis-element of the genome, display cell cycle stage-dependent dynamics in factor binding and nucleosome positioning.

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Posted July 11, 2018.
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CTCF sites display cell cycle dependent dynamics in factor binding and nucleosome positioning
Marlies E. Oomen, Anders S. Hansen, Yu Liu, Xavier Darzacq, Job Dekker
bioRxiv 365866; doi: https://doi.org/10.1101/365866
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CTCF sites display cell cycle dependent dynamics in factor binding and nucleosome positioning
Marlies E. Oomen, Anders S. Hansen, Yu Liu, Xavier Darzacq, Job Dekker
bioRxiv 365866; doi: https://doi.org/10.1101/365866

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