Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

C. elegans Mediator 15 permits low temperature-induced longevity via regulation of lipid and protein homeostasis

Dongyeop Lee, Seon Woo A. An, Yoonji Jung, Yasuyo Yamaoka, Youngjae Ryu, Grace Ying Shyen Goh, Arshia Beigi, Dengke Ma, Chang Man Ha, Stefan Taubert, Youngsook Lee, Seung-Jae V. Lee
doi: https://doi.org/10.1101/366153
Dongyeop Lee
Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea, 37673
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Seon Woo A. An
Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea, 37673
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yoonji Jung
Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea, 37673
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yasuyo Yamaoka
Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea, 37673
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Youngjae Ryu
Research Division, Korea Brain Research Institute, Daegu, South Korea, 41068
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Grace Ying Shyen Goh
Centre for Molecular Medicine and Therapeutics, BC Children’s Hospital Research Institute, Department of Medical Genetics, University of British Columbia, 950 W 28th Ave, Vancouver, BC V5Z 4H4, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Arshia Beigi
Centre for Molecular Medicine and Therapeutics, BC Children’s Hospital Research Institute, Department of Medical Genetics, University of British Columbia, 950 W 28th Ave, Vancouver, BC V5Z 4H4, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dengke Ma
Cardiovascular Research Institute and Department of Physiology, University of California San Francisco, San Francisco, CA, USA, 94158
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chang Man Ha
Research Division, Korea Brain Research Institute, Daegu, South Korea, 41068
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stefan Taubert
Centre for Molecular Medicine and Therapeutics, BC Children’s Hospital Research Institute, Department of Medical Genetics, University of British Columbia, 950 W 28th Ave, Vancouver, BC V5Z 4H4, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Youngsook Lee
Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea, 37673Department of Integrative Bioscience & Biotechnology, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea, 37673
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Seung-Jae V. Lee
Department of Life Sciences, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea, 37673School of Interdisciplinary Bioscience and Bioengineering, and Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea, 37673
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: seungjaelee@postech.ac.kr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Low temperatures slow aging and extend lifespan in many organisms, including Caenorhabditis elegans. However, the metabolic and homeostatic aspects of low temperature-induced longevity remain poorly understood. Here, we show that changes in lipid composition regulated by MDT-15/Mediator 15, transcriptional co-regulator, is essential for low temperature-induced longevity and proteostasis in C. elegans. We find that inhibition of mdt-15 prevents animals from living long at low temperatures. We show that MDT-15 up-regulates fat-7, a fatty acid desaturase, at low temperatures, which increases the ratio of unsaturated to saturated fatty acids. We further demonstrate that maintaining this increased fatty acid ratio is essential for protein homeostasis and longevity at low temperatures. Thus, the homeostasis of lipid composition by MDT-15 appears to be a limiting factor for C. elegans proteostasis and longevity at low temperatures. Our findings highlight the crucial roles of fat regulation in maintaining normal organismal physiology under different environmental conditions.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted July 10, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
C. elegans Mediator 15 permits low temperature-induced longevity via regulation of lipid and protein homeostasis
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
C. elegans Mediator 15 permits low temperature-induced longevity via regulation of lipid and protein homeostasis
Dongyeop Lee, Seon Woo A. An, Yoonji Jung, Yasuyo Yamaoka, Youngjae Ryu, Grace Ying Shyen Goh, Arshia Beigi, Dengke Ma, Chang Man Ha, Stefan Taubert, Youngsook Lee, Seung-Jae V. Lee
bioRxiv 366153; doi: https://doi.org/10.1101/366153
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
C. elegans Mediator 15 permits low temperature-induced longevity via regulation of lipid and protein homeostasis
Dongyeop Lee, Seon Woo A. An, Yoonji Jung, Yasuyo Yamaoka, Youngjae Ryu, Grace Ying Shyen Goh, Arshia Beigi, Dengke Ma, Chang Man Ha, Stefan Taubert, Youngsook Lee, Seung-Jae V. Lee
bioRxiv 366153; doi: https://doi.org/10.1101/366153

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (1545)
  • Biochemistry (2500)
  • Bioengineering (1757)
  • Bioinformatics (9729)
  • Biophysics (3929)
  • Cancer Biology (2990)
  • Cell Biology (4235)
  • Clinical Trials (135)
  • Developmental Biology (2653)
  • Ecology (4129)
  • Epidemiology (2033)
  • Evolutionary Biology (6933)
  • Genetics (5243)
  • Genomics (6532)
  • Immunology (2208)
  • Microbiology (7012)
  • Molecular Biology (2784)
  • Neuroscience (17412)
  • Paleontology (127)
  • Pathology (432)
  • Pharmacology and Toxicology (712)
  • Physiology (1068)
  • Plant Biology (2516)
  • Scientific Communication and Education (647)
  • Synthetic Biology (835)
  • Systems Biology (2699)
  • Zoology (439)