Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A cell cycle-coordinated nuclear compartment for Polymerase II transcription encompasses the earliest gene expression before global genome activation

Yavor Hadzhiev, Haseeb K. Qureshi, Lucy Wheatley, Ledean Cooper, Aleksandra Jasiulewicz, Huy Van Nguyen, Joseph Wragg, Divyasree Poovathumkadavil, Sascha Conic, Sarah Bajan, View ORCID ProfileAttila Sik, View ORCID ProfileGyörgy Hutvàgner, View ORCID ProfileLàszlò Tora, Agnieszka Gambus, View ORCID ProfileJohn S. Fossey, View ORCID ProfileFerenc Müller
doi: https://doi.org/10.1101/366468
Yavor Hadzhiev
1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Haseeb K. Qureshi
1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lucy Wheatley
1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ledean Cooper
1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aleksandra Jasiulewicz
1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Huy Van Nguyen
2School of Chemistry, College of Environmental and Physical Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joseph Wragg
1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Divyasree Poovathumkadavil
1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sascha Conic
3Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Centre National de la Recherche Scientifique, UMR7104, Institut National de la Santé et de la Recherche Médicale, U964, Université de Strasbourg, 67404 Illkirch, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sarah Bajan
4Centre for Heath Technologies, University of Technology, Sydney, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Attila Sik
5Szentagothai Research Centre, Medical School, University of Pecs, Pecs, Hungary
6Institute of Clinical Science, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Attila Sik
György Hutvàgner
4Centre for Heath Technologies, University of Technology, Sydney, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for György Hutvàgner
Làszlò Tora
3Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Centre National de la Recherche Scientifique, UMR7104, Institut National de la Santé et de la Recherche Médicale, U964, Université de Strasbourg, 67404 Illkirch, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Làszlò Tora
Agnieszka Gambus
1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John S. Fossey
2School of Chemistry, College of Environmental and Physical Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for John S. Fossey
Ferenc Müller
1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ferenc Müller
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Most metazoan embryos commence development with rapid cleavages without zygotic gene expression and their genome activation is delayed until the mid-blastula transition (MBT). However, a set of genes escape global repression during the extremely fast cell cycles, which lack gap phases and their transcription is activated before the MBT. Here we describe the formation and the spatio-temporal dynamics of a distinct transcription compartment, which encompasses the earliest detectable transcription during the first wave of genome activation. Simultaneous 4D imaging of expression of pri-miR430 and zinc finger genes by a novel, native transcription imaging approach reveals a pair of shared transcription compartments regulated by homolog chromosome organisation. These nuclear compartments carry the majority of nascent RNAs and transcriptionally active Polymerase II, are depleted of compact chromatin and represent the main sites for detectable transcription before MBT. We demonstrate that transcription occurs in the S-phase of the cleavage cycles and that the gradual slowing of these cell cycles are permissive to transcription before global genome activation. We propose that the demonstrated transcription compartment is part of the regulatory architecture of nucleus organisation, and provides a transcriptionally competent, supporting environment to facilitate early escape from the general nuclear repression before global genome activation.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted July 15, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A cell cycle-coordinated nuclear compartment for Polymerase II transcription encompasses the earliest gene expression before global genome activation
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A cell cycle-coordinated nuclear compartment for Polymerase II transcription encompasses the earliest gene expression before global genome activation
Yavor Hadzhiev, Haseeb K. Qureshi, Lucy Wheatley, Ledean Cooper, Aleksandra Jasiulewicz, Huy Van Nguyen, Joseph Wragg, Divyasree Poovathumkadavil, Sascha Conic, Sarah Bajan, Attila Sik, György Hutvàgner, Làszlò Tora, Agnieszka Gambus, John S. Fossey, Ferenc Müller
bioRxiv 366468; doi: https://doi.org/10.1101/366468
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
A cell cycle-coordinated nuclear compartment for Polymerase II transcription encompasses the earliest gene expression before global genome activation
Yavor Hadzhiev, Haseeb K. Qureshi, Lucy Wheatley, Ledean Cooper, Aleksandra Jasiulewicz, Huy Van Nguyen, Joseph Wragg, Divyasree Poovathumkadavil, Sascha Conic, Sarah Bajan, Attila Sik, György Hutvàgner, Làszlò Tora, Agnieszka Gambus, John S. Fossey, Ferenc Müller
bioRxiv 366468; doi: https://doi.org/10.1101/366468

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3513)
  • Biochemistry (7359)
  • Bioengineering (5338)
  • Bioinformatics (20306)
  • Biophysics (10034)
  • Cancer Biology (7763)
  • Cell Biology (11331)
  • Clinical Trials (138)
  • Developmental Biology (6444)
  • Ecology (9968)
  • Epidemiology (2065)
  • Evolutionary Biology (13346)
  • Genetics (9365)
  • Genomics (12598)
  • Immunology (7718)
  • Microbiology (19059)
  • Molecular Biology (7452)
  • Neuroscience (41106)
  • Paleontology (300)
  • Pathology (1233)
  • Pharmacology and Toxicology (2141)
  • Physiology (3171)
  • Plant Biology (6869)
  • Scientific Communication and Education (1275)
  • Synthetic Biology (1899)
  • Systems Biology (5320)
  • Zoology (1090)